GISZF001

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Result Filters

Organism: Danio rerio (zebrafish)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Otomorpha; Ostariophysi; Otophysi; Cypriniphysae; Cypriniformes; Cyprinoidei; Danionidae; Danioninae; Danio

Attributes

tissue	Embryo
development stage	7 Different embryonic Stages( From just fertilized Embryos to 72 hours just hatched baby fish)
lab host	DH10B
vector	pDNR-LIB
v_type	Plasmid
re_1	Sfi A (GGCCATTACGGCC)
re_2	Sfi B (GGCCGCCTCGGCC)

Description: Priming method: Sfi-(dT)30 Primed ; Priming sequence: 5.ATTCTAGA GGCCGAGGCGGCC GACATG(T)30VN ; Directionally cloned, 5' cloning site: Sfi A site GGCCATTACGGCC ; 5' linker/adaptor sequence: 5.AAGCAGTGGTATCAACGCAGAGTGGCC ; 3' cloning site: Sfi B site GGCCGCCTCGGCC ; 3' linker/adaptor sequence: same as the priming sequence ; Average insert size: 2kb ; For PCR insert analysis: Use M13 Forward and reverse primers ; Library Amplified Recombinants (inserts): 98% ; Library complexity: 5x106 ; Full-length construction (method): SMART, a Clontech method ; Library constructed by: S. Mathavan, Chia-Lin Wei, and Yijun Ruan Genome Institute of Singapore