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NCI_CGAP_FE0 is a cDNA library containing the following tissue(s): a pool of 3 chondrosarcoma cell lines ( grade 2) The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CGCTACGGAC.The cell lines was provided by Dr James Martin of University of Iowa.
Nucleotide
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