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1st strand cDNA was primed with a Not I - oligo(dT) primer [5'-CAGCGGCCGCTTTTTTTTTTTTTTTTTTV-3']; double-stranded cDNA was ligated to Sal I adaptors [5'-TCGACCCACGCGTCCG-3'], digested with Not I and cloned into the Sal I and Not I sites of pSPORT 1 vector.; Average insert size: 0.7 kb (range 0.2-1.3 kb); Sequence primer: T7 [5'-TAATACGACTCACTATAGGG-3']; Primary library, non-amplified.
Nucleotide
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