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Poly A+ RNAs were extracted from circumvallate taste papillae (tester) and non-taste lingual epithelium (driver). Following a pre-amplification step using the Smart PCR cDNA Synthesis kit (K1052-1, BD Biosciences), circumvallate papillae cDNA was subtracted against non-taste lingual epithelium cDNA using the PCR-Select cDNA subtraction kit (K1804-1, BD Biosciences) according to manufacturer's protocols, which are available in detail at http://www.clontech.com/. Subtracted cDNAs were non-directionally cloned into the T/A cloning site of pCRII (Invitrogen). The sequencing of individual clones, and EST analysis were performed at the NIH Intramural Sequencing Center (NISC). Analyzed data available through http://neibank.nei.nih.gov.
Nucleotide
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