McCarrey Eddy type B spermatogonia

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Result Filters

Organism: Mus musculus (house mouse)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus

Attributes

strain	CD-1
sex	male
tissue	type B spermatogonia, pooled from multiple mice
development stage	8 day
lab host	DH10B (phage-resistant)
tissue	testis
vector	pBluescript SK+ (Stratagene)
v_type	phagemid (ampicillin resistant)
re_1	XhoI
re_2	EcoRI

Description: cDNA oligo dT-primed [5'-(GA)10-ACTAGTCTCGAGTTTTTTTTTTTTT-3'] and directionally cloned using 5' linkers 5'-AATTCGGCACGAG-3' and 5'-CTCGTGCCG-3'. Size selection of >400bp material gives average insert size ranging from 1-2 kb. Library was mass excised (from lambda-UniZAP-XR) and resulting single-stranded phagemids were prepped and tranformed into DH10B. Library contains 96% recombinants. References: J. Androl. 20:635-639 and Gene 25:263-269. Library constructed and donated by J. McCarrey, Ph.D. (Southwest Foundation for Biomedical Research, Dept. of Genetics); excision done by E.M. Eddy, Ph.D. (National Institutes of Health, National Institute of Environmental Health Sciences). Original lambda-based library is available through ATCC, catalog #63417.