BioProject

To investigate the functional conservation of H2A.Z histones during eukaryotic evolution we transformed h2a. More...

To investigate the functional conservation of H2A.Z histones during eukaryotic evolution we transformed h2a.z deficient plants with three human H2A.Z proteins to assess their ability to rescue the mutant defects. We discovered that human H2A.Z.1 and H2A.Z.2.1 fully complement the phenotypic abnormalities of h2a.z plants even though Arabidopsis and human H2A.Z N-terminal tail sequences are divergent, while the brain-specific splice variant H2A.Z.2.2 has a dominant-negative effect in wild-type plants. Furthermore, H2A.Z.1 almost completely re-establishes normal H2A.Z chromatin occupancy in h2a.z plants and restores the expression of more than 84 % of misexpressed genes. Finally, our hypothesis that the N-terminal tail of Arabidopsis H2A.Z is not crucial for its overall function was supported by the ability of N-terminal end truncations of Arabidopsis HTA11 to largely rescue the defects of h2a.z mutants. Overall design: RNA-seq from total RNA of the following plants: WT, h2a.z, h2a.z + AtHTA11. For h2a.z plants, aboveground tissue from three different plants was harvested and individually processed to extract total RNA. For all other genotypes, five individual leaves (third or fourth pair of rosette leaves) were collected from 5 different plants. The leaves were ground in liquid nitrogen and split into three equal replicates to extract total RNA. Less...