BioProject

Hypertrophic scar (HS) considerably affects the appearance and causes tissue dysfunction in patients. More...

Hypertrophic scar (HS) considerably affects the appearance and causes tissue dysfunction in patients. Here we show a separating microneedle (MN) consisting of photo-crosslinked GelMA and 5-FuA-Pep-MA prodrug in response to high reactive oxygen species (ROS) levels and overexpression of matrix metalloproteinases (MMPs) in the HS pathological microenvironment. RNA sequencing analyses confirm that drug-loaded MNs could reverse skin fibrosis through down-regulation of BCL-2-associated death promoter (BAD), insulin-like growth factor 1 receptor (IGF1R) pathways, simultaneously regulate inflammatory response and keratinocyte differentiation via up-regulation of toll-like receptors (TOLL), interleukin-1 receptor (IL1R) and keratinocyte pathways. Overall design: To elucidate the RNA changes associated with drug-loaded MNs on HS, we performed RNA sequencing (RNA-seq) analysis using rabbit ear normal skin (NS), rabbit ear HS tissues, HS tissues with drug-loaded MNs, and HS tissues with 5-Fu injection treatment. Comparative gene expression profiling analysis of RNA-seq data for HS versus NS, HS with drug-loaded MNs versus HS, and HS with 5-Fu injection versus HS. Less...