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Conserved domains on  [gi|2117994302|ref|XP_044289465|]
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RNA demethylase ALKBH5 [Varanus komodoensis]

Protein Classification

2OG-Fe(II) oxygenase family protein( domain architecture ID 229412)

2OG-Fe(II) oxygenase family protein belongs to the large and diverse Fe(II)- and 2-oxoglutarate (2-OG)-dependent dioxygenase superfamily and may share a common reaction mechanism, using Fe(II) and the cosubstrate 2-OG in the active site to activate oxygen, resulting in the two-electron oxidation of a target substrate

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
2OG-FeII_Oxy super family cl21496
2OG-Fe(II) oxygenase superfamily; This family contains members of the 2-oxoglutarate (2OG) and ...
 82-258 1.85e-10

2OG-Fe(II) oxygenase superfamily; This family contains members of the 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily. This family includes the C-terminal of prolyl 4-hydroxylase alpha subunit. The holoenzyme has the activity EC:1.14.11.2 catalysing the reaction: Procollagen L-proline + 2-oxoglutarate + O2 <=> procollagen trans- 4-hydroxy-L-proline + succinate + CO2. The full enzyme consists of a alpha2 beta2 complex with the alpha subunit contributing most of the parts of the active site. The family also includes lysyl hydrolases, isopenicillin synthases and AlkB.


The actual alignment was detected with superfamily member pfam13532:

Pssm-ID: 473886  Cd Length: 191  Bit Score: 59.64  E-value: 1.85e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2117994302  82 LFSPEECAKIEARIDEvvsraEKGLYKEHTVDRAPLRNKY-FFGEGYTYGSQLQRRGPGQERLYPRGdVDPIPEWVhaLV 160
Cdd:pfam13532   8 FLSPEEAAELLRELLE-----EGPFRQPTTQGGRPMSVRMtNCGQLGWVTDGPGYRYSGVDPVTGEP-WPPFPEAL--LQ 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2117994302 161 IRKLVERRVIPEGF-VNSAVINDYQPGGCIVSHVDPIHI-FERPIVSVSffsdsalcFGCK--FQFKPIRVSEPVFFLPV 236
Cdd:pfam13532  80 LAERLAAEAGYPGWsPNACLVNFYRDGARMGLHQDRDESgPGAPIVSLS--------LGASatFRFGGKSRSDPTISLRL 151

                         170       180
                  ....*....|....*....|..
gi 2117994302 237 RRGSVTVLSGyAADEITHCIRP 258
Cdd:pfam13532 152 ESGDVLVMGG-ESRLAYHGVPP 172
 
Name Accession Description Interval E-value
2OG-FeII_Oxy_2 pfam13532
2OG-Fe(II) oxygenase superfamily;
82-258 1.85e-10

2OG-Fe(II) oxygenase superfamily;


Pssm-ID: 433285  Cd Length: 191  Bit Score: 59.64  E-value: 1.85e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2117994302  82 LFSPEECAKIEARIDEvvsraEKGLYKEHTVDRAPLRNKY-FFGEGYTYGSQLQRRGPGQERLYPRGdVDPIPEWVhaLV 160
Cdd:pfam13532   8 FLSPEEAAELLRELLE-----EGPFRQPTTQGGRPMSVRMtNCGQLGWVTDGPGYRYSGVDPVTGEP-WPPFPEAL--LQ 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2117994302 161 IRKLVERRVIPEGF-VNSAVINDYQPGGCIVSHVDPIHI-FERPIVSVSffsdsalcFGCK--FQFKPIRVSEPVFFLPV 236
Cdd:pfam13532  80 LAERLAAEAGYPGWsPNACLVNFYRDGARMGLHQDRDESgPGAPIVSLS--------LGASatFRFGGKSRSDPTISLRL 151

                         170       180
                  ....*....|....*....|..
gi 2117994302 237 RRGSVTVLSGyAADEITHCIRP 258
Cdd:pfam13532 152 ESGDVLVMGG-ESRLAYHGVPP 172
AlkB COG3145
Alkylated DNA repair dioxygenase AlkB [Replication, recombination and repair];
151-246 1.09e-03

Alkylated DNA repair dioxygenase AlkB [Replication, recombination and repair];


Pssm-ID: 442379  Cd Length: 200  Bit Score: 39.76  E-value: 1.09e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2117994302 151 PIPEWVHALviRKLVERRVipeGFV-NSAVINDYQPGGCIVS-HVD--PIHIFERPIVSVSFfsdsalcfG--CKFQFKP 224
Cdd:COG3145    82 PWPPALLAL--KRRVEAAA---GFPfNSCLLNLYRDGQDRMGwHQDdeEELGFNPPIASVSL--------GatRRFRFGH 148

                          90       100
                  ....*....|....*....|..
gi 2117994302 225 IRVSEPVFFLPVRRGSVTVLSG 246
Cdd:COG3145   149 KERKDPTRSLPLEHGDLLVMGG 170
 
Name Accession Description Interval E-value
2OG-FeII_Oxy_2 pfam13532
2OG-Fe(II) oxygenase superfamily;
82-258 1.85e-10

2OG-Fe(II) oxygenase superfamily;


Pssm-ID: 433285  Cd Length: 191  Bit Score: 59.64  E-value: 1.85e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2117994302  82 LFSPEECAKIEARIDEvvsraEKGLYKEHTVDRAPLRNKY-FFGEGYTYGSQLQRRGPGQERLYPRGdVDPIPEWVhaLV 160
Cdd:pfam13532   8 FLSPEEAAELLRELLE-----EGPFRQPTTQGGRPMSVRMtNCGQLGWVTDGPGYRYSGVDPVTGEP-WPPFPEAL--LQ 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2117994302 161 IRKLVERRVIPEGF-VNSAVINDYQPGGCIVSHVDPIHI-FERPIVSVSffsdsalcFGCK--FQFKPIRVSEPVFFLPV 236
Cdd:pfam13532  80 LAERLAAEAGYPGWsPNACLVNFYRDGARMGLHQDRDESgPGAPIVSLS--------LGASatFRFGGKSRSDPTISLRL 151

                         170       180
                  ....*....|....*....|..
gi 2117994302 237 RRGSVTVLSGyAADEITHCIRP 258
Cdd:pfam13532 152 ESGDVLVMGG-ESRLAYHGVPP 172
AlkB COG3145
Alkylated DNA repair dioxygenase AlkB [Replication, recombination and repair];
151-246 1.09e-03

Alkylated DNA repair dioxygenase AlkB [Replication, recombination and repair];


Pssm-ID: 442379  Cd Length: 200  Bit Score: 39.76  E-value: 1.09e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2117994302 151 PIPEWVHALviRKLVERRVipeGFV-NSAVINDYQPGGCIVS-HVD--PIHIFERPIVSVSFfsdsalcfG--CKFQFKP 224
Cdd:COG3145    82 PWPPALLAL--KRRVEAAA---GFPfNSCLLNLYRDGQDRMGwHQDdeEELGFNPPIASVSL--------GatRRFRFGH 148

                          90       100
                  ....*....|....*....|..
gi 2117994302 225 IRVSEPVFFLPVRRGSVTVLSG 246
Cdd:COG3145   149 KERKDPTRSLPLEHGDLLVMGG 170
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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