|
Name |
Accession |
Description |
Interval |
E-value |
| mito_nad_idh |
TIGR00175 |
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent ... |
1-325 |
0e+00 |
|
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the multimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Mitochondrial NAD-dependent isocitrate dehydrogenases (IDH) resemble prokaryotic NADP-dependent IDH and 3-isopropylmalate dehydrogenase (an NAD-dependent enzyme) more closely than they resemble eukaryotic NADP-dependent IDH. The mitochondrial NAD-dependent isocitrate dehydrogenase is believed to be an alpha(2)-beta-gamma heterotetramer. All subunits are homologous and found by this model. The NADP-dependent IDH of Thermus aquaticus thermophilus strain HB8 resembles these NAD-dependent IDH, except for the residues involved in cofactor specificity, much more closely than it resembles other prokaryotic NADP-dependent IDH, including that of Thermus aquaticus strain YT1. [Energy metabolism, TCA cycle]
Pssm-ID: 272942 Cd Length: 333 Bit Score: 565.47 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 1 MIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNAD--EEDIRNAIMAIRRNRVALKGNIETNHNLPpSHKSRNNILR 78
Cdd:TIGR00175 8 LIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDgkTEIPDEAVESIKRNKVALKGPLETPIGKG-GHRSLNVALR 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 79 TSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKV 158
Cdd:TIGR00175 87 KELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYARKNGRKKV 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 159 TAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAG 238
Cdd:TIGR00175 167 TAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVGGPGLVPG 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 239 ANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSQAI 318
Cdd:TIGR00175 247 ANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTKDLGGTATTSDFT 326
|
....*..
gi 1039796369 319 QDIIRHI 325
Cdd:TIGR00175 327 EAVIKRL 333
|
|
| PLN00123 |
PLN00123 |
isocitrate dehydrogenase (NAD+) |
1-325 |
5.05e-127 |
|
isocitrate dehydrogenase (NAD+)
Pssm-ID: 215065 Cd Length: 360 Bit Score: 367.65 E-value: 5.05e-127
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 1 MIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSnaDEEDIRNAIM-AIRRNRVALKGNIETNHNLPPShkSRNNILRT 79
Cdd:PLN00123 35 LIPGDGIGPLVTGAVEQVMEAMHAPVYFERYEVHG--DMKKVPEEVLeSIRRNKVCLKGGLATPVGGGVS--SLNVQLRK 110
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 80 SLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVT 159
Cdd:PLN00123 111 ELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNNRKKVT 190
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 160 AVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGA 239
Cdd:PLN00123 191 AVHKANIMKLADGLFLESCREVAKKYPGIKYNEIIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGG 270
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 240 NYGHVYAVFETATR--NTGK-SIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSQ 316
Cdd:PLN00123 271 NVGADHAVFEQGASagNVGNeKLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGKYRTKDLGGSSTTQE 350
|
....*....
gi 1039796369 317 AIQDIIRHI 325
Cdd:PLN00123 351 VVDAVIANL 359
|
|
| LeuB |
COG0473 |
Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid ... |
2-325 |
1.01e-116 |
|
Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid transport and metabolism]; Isocitrate/isopropylmalate dehydrogenase is part of the Pathway/BioSystem: TCA cycle
Pssm-ID: 440241 Cd Length: 346 Bit Score: 340.84 E-value: 1.01e-116
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 2 IPGDGIGPELMLHVKSVFRHAC----VPVDFEEVHVSSNA--------DEEDIRnaimAIRRNRVALKGNIETNHnlPPS 69
Cdd:COG0473 7 LPGDGIGPEVVAAALKVLEAAAerfgLDFEFEEADIGGAAydktgtplPDETLE----ALRKADAILLGAVGGPK--WDD 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 70 HKSRNNI---LRTSLDLYANVIHCKSLPGVVTRHK-----DIDILIVRENTEGEYSSLEHESVAG----VVESLKIITKA 137
Cdd:COG0473 81 GVRPESGllaLRKELDLYANLRPAKLYPGLPSPLKpeiveGIDLVIVRENTEGLYFGIGGRIGTGtgeeVAIDTRVYTRK 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 138 KSLRIAEYAFKLAQESgRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNL 217
Cdd:COG0473 161 GIERIARYAFELARKR-RKKVTSVDKANVLKLTSGLWREVVREVAKEYPDVELDHMYVDAAAMQLVRNPEQFDVIVTENL 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 218 YGNIVNNVCAGLVGGPGLVAGANYG-HVYAVFE----TATRntgksIANKNIANPTATLLASCMMLDHLKLHSYATSIRK 292
Cdd:COG0473 240 FGDILSDLAAGLTGSLGLAPSANIGdEGKALFEpvhgSAPD-----IAGKGIANPIATILSAAMMLRHLGEEEAADAIEA 314
|
330 340 350
....*....|....*....|....*....|...
gi 1039796369 293 AVLASMDnENMHTPDIGGQGTTSQAIQDIIRHI 325
Cdd:COG0473 315 AVEKVLA-EGVRTPDLGGKAGTSEMGDAIIAAL 346
|
|
| Iso_dh |
pfam00180 |
Isocitrate/isopropylmalate dehydrogenase; |
2-321 |
1.75e-88 |
|
Isocitrate/isopropylmalate dehydrogenase;
Pssm-ID: 425507 Cd Length: 346 Bit Score: 269.16 E-value: 1.75e-88
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 2 IPGDGIGPELMLHVKSVFRHACVPV----DFEEVHVSSNA-DEEDI---RNAIMAIRRNRVALKGNIETNHNLPPSHKSR 73
Cdd:pfam00180 5 LPGDGIGPEVMAAALKVLKAALEKAplefEFEERDVGGAAiDETGEplpDETLEACKKADAVLLGAVGGPKWDPAGVRPE 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 74 NNI--LRTSLDLYANVIHCKSLPGVVTRHK------DIDILIVRENTEGEYSSLEHE---SVAGVVESLKIITKAKSLRI 142
Cdd:pfam00180 85 NGLlaLRKELGLFANLRPAKVFPPLGDASPlkneveGVDIVIVRELTGGIYFGIEKGikgSGNEVAVDTKLYSRDEIERI 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 143 AEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIV 222
Cdd:pfam00180 165 ARVAFELARKRGRKKVTSVDKANVLKSSDLWRKIVTEVAKAEYPDVELEHQLVDNAAMQLVKNPSQFDVIVTPNLFGDIL 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 223 NNVCAGLVGGPGLVAGANYG-HVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDH-LKLHSYATSIRKAVLASMDN 300
Cdd:pfam00180 245 SDEASMLTGSLGLLPSASLGaNGFGIFE-PVHGSAPDIAGKGIANPIATILSAAMMLRYsLGLEDAADKIEAAVLKVLES 323
|
330 340
....*....|....*....|....
gi 1039796369 301 ENMhTPDIGGQGT---TSQAIQDI 321
Cdd:pfam00180 324 GIR-TGDLAGSATyvsTSEFGEAV 346
|
|
| |
|
Name |
Accession |
Description |
Interval |
E-value |
| mito_nad_idh |
TIGR00175 |
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent ... |
1-325 |
0e+00 |
|
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the multimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Mitochondrial NAD-dependent isocitrate dehydrogenases (IDH) resemble prokaryotic NADP-dependent IDH and 3-isopropylmalate dehydrogenase (an NAD-dependent enzyme) more closely than they resemble eukaryotic NADP-dependent IDH. The mitochondrial NAD-dependent isocitrate dehydrogenase is believed to be an alpha(2)-beta-gamma heterotetramer. All subunits are homologous and found by this model. The NADP-dependent IDH of Thermus aquaticus thermophilus strain HB8 resembles these NAD-dependent IDH, except for the residues involved in cofactor specificity, much more closely than it resembles other prokaryotic NADP-dependent IDH, including that of Thermus aquaticus strain YT1. [Energy metabolism, TCA cycle]
Pssm-ID: 272942 Cd Length: 333 Bit Score: 565.47 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 1 MIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNAD--EEDIRNAIMAIRRNRVALKGNIETNHNLPpSHKSRNNILR 78
Cdd:TIGR00175 8 LIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDgkTEIPDEAVESIKRNKVALKGPLETPIGKG-GHRSLNVALR 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 79 TSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKV 158
Cdd:TIGR00175 87 KELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYARKNGRKKV 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 159 TAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAG 238
Cdd:TIGR00175 167 TAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVGGPGLVPG 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 239 ANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSQAI 318
Cdd:TIGR00175 247 ANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTKDLGGTATTSDFT 326
|
....*..
gi 1039796369 319 QDIIRHI 325
Cdd:TIGR00175 327 EAVIKRL 333
|
|
| PLN00123 |
PLN00123 |
isocitrate dehydrogenase (NAD+) |
1-325 |
5.05e-127 |
|
isocitrate dehydrogenase (NAD+)
Pssm-ID: 215065 Cd Length: 360 Bit Score: 367.65 E-value: 5.05e-127
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 1 MIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSnaDEEDIRNAIM-AIRRNRVALKGNIETNHNLPPShkSRNNILRT 79
Cdd:PLN00123 35 LIPGDGIGPLVTGAVEQVMEAMHAPVYFERYEVHG--DMKKVPEEVLeSIRRNKVCLKGGLATPVGGGVS--SLNVQLRK 110
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 80 SLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVT 159
Cdd:PLN00123 111 ELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNNRKKVT 190
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 160 AVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGA 239
Cdd:PLN00123 191 AVHKANIMKLADGLFLESCREVAKKYPGIKYNEIIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGG 270
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 240 NYGHVYAVFETATR--NTGK-SIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSQ 316
Cdd:PLN00123 271 NVGADHAVFEQGASagNVGNeKLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGKYRTKDLGGSSTTQE 350
|
....*....
gi 1039796369 317 AIQDIIRHI 325
Cdd:PLN00123 351 VVDAVIANL 359
|
|
| PLN00118 |
PLN00118 |
isocitrate dehydrogenase (NAD+) |
1-325 |
1.22e-123 |
|
isocitrate dehydrogenase (NAD+)
Pssm-ID: 215062 Cd Length: 372 Bit Score: 359.58 E-value: 1.22e-123
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 1 MIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEED----IRNAIMAIRRNRVALKGNIETNhnLPPSHKSRNNI 76
Cdd:PLN00118 46 LFPGDGIGPEIAESVKQVFTAAGVPIEWEEHYVGTTVDPRTgsflTWESLESVRRNKVGLKGPMATP--IGKGHRSLNLT 123
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 77 LRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRK 156
Cdd:PLN00118 124 LRKELGLYANVRPCYSLPGYKTRYDDVDLVTIRENTEGEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTHGRK 203
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 157 KVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLV 236
Cdd:PLN00118 204 RVSAIHKANIMKKTDGLFLKCCREVAEKYPEIVYEEVIIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLT 283
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 237 AGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSQ 316
Cdd:PLN00118 284 PSCNIGENGLALAEAVHGSAPDIAGKNLANPTALLLSAVMMLRHLKLNEQAEQIHNAILNTIAEGKYRTADLGGSSTTTD 363
|
....*....
gi 1039796369 317 AIQDIIRHI 325
Cdd:PLN00118 364 FTKAICDHL 372
|
|
| LeuB |
COG0473 |
Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid ... |
2-325 |
1.01e-116 |
|
Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid transport and metabolism]; Isocitrate/isopropylmalate dehydrogenase is part of the Pathway/BioSystem: TCA cycle
Pssm-ID: 440241 Cd Length: 346 Bit Score: 340.84 E-value: 1.01e-116
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 2 IPGDGIGPELMLHVKSVFRHAC----VPVDFEEVHVSSNA--------DEEDIRnaimAIRRNRVALKGNIETNHnlPPS 69
Cdd:COG0473 7 LPGDGIGPEVVAAALKVLEAAAerfgLDFEFEEADIGGAAydktgtplPDETLE----ALRKADAILLGAVGGPK--WDD 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 70 HKSRNNI---LRTSLDLYANVIHCKSLPGVVTRHK-----DIDILIVRENTEGEYSSLEHESVAG----VVESLKIITKA 137
Cdd:COG0473 81 GVRPESGllaLRKELDLYANLRPAKLYPGLPSPLKpeiveGIDLVIVRENTEGLYFGIGGRIGTGtgeeVAIDTRVYTRK 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 138 KSLRIAEYAFKLAQESgRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNL 217
Cdd:COG0473 161 GIERIARYAFELARKR-RKKVTSVDKANVLKLTSGLWREVVREVAKEYPDVELDHMYVDAAAMQLVRNPEQFDVIVTENL 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 218 YGNIVNNVCAGLVGGPGLVAGANYG-HVYAVFE----TATRntgksIANKNIANPTATLLASCMMLDHLKLHSYATSIRK 292
Cdd:COG0473 240 FGDILSDLAAGLTGSLGLAPSANIGdEGKALFEpvhgSAPD-----IAGKGIANPIATILSAAMMLRHLGEEEAADAIEA 314
|
330 340 350
....*....|....*....|....*....|...
gi 1039796369 293 AVLASMDnENMHTPDIGGQGTTSQAIQDIIRHI 325
Cdd:COG0473 315 AVEKVLA-EGVRTPDLGGKAGTSEMGDAIIAAL 346
|
|
| PRK08997 |
PRK08997 |
isocitrate dehydrogenase; Provisional |
1-325 |
6.77e-106 |
|
isocitrate dehydrogenase; Provisional
Pssm-ID: 181606 Cd Length: 334 Bit Score: 313.19 E-value: 6.77e-106
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 1 MIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDI----RNAIMAIRRNRVALKGNIETNhnLPPSHKSRNNI 76
Cdd:PRK08997 7 VIPGDGIGPSIIDATLKILDKLGCDFEYEFADAGLTALEKHGellpQRTLDLIEKNKIALKGPLTTP--VGEGFTSINVT 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 77 LRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHE-SVAG-VVESLKIITKAKSLRIAEYAFKLAQESG 154
Cdd:PRK08997 85 LRKKFDLYANVRPVLSFPGTKARYDNIDIITVRENTEGMYSGEGQTvSEDGeTAEATSIITRKGAERIVRFAYELARKEG 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 155 RKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPG 234
Cdd:PRK08997 165 RKKVTAVHKANIMKSTSGLFLKVAREVALRYPDIEFEEMIVDATCMQLVMNPEQFDVIVTTNLFGDILSDLCAGLVGGLG 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 235 LVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTT 314
Cdd:PRK08997 245 MAPGANIGRDAAIFE-AVHGSAPDIAGKNLANPTSVILAAIQMLEYLGMPDKAERIRKAIVAVIEAGDRTTRDLGGTHGT 323
|
330
....*....|.
gi 1039796369 315 SQAIQDIIRHI 325
Cdd:PRK08997 324 TDFTQAVIDRL 334
|
|
| PRK09222 |
PRK09222 |
NADP-dependent isocitrate dehydrogenase; |
2-316 |
4.08e-99 |
|
NADP-dependent isocitrate dehydrogenase;
Pssm-ID: 236416 [Multi-domain] Cd Length: 482 Bit Score: 300.65 E-value: 4.08e-99
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 2 IPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIM-----AIRRNRVALKGNIETnhnlpP---SHKSR 73
Cdd:PRK09222 10 AYGDGIGPEIMEAVLKILEAAGAPLEIETIEIGEKVYKKGWTSGISpsaweSIRRTKVLLKAPITT-----PqggGYKSL 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 74 NNILRTSLDLYANVIHCKSL-PGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQE 152
Cdd:PRK09222 85 NVTLRKTLGLYANVRPCVSYhPFVETKHPNLDVVIIRENEEDLYAGIEHRQTPDVYQCLKLISRPGSEKIIRYAFEYARA 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 153 SGRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGG 232
Cdd:PRK09222 165 NGRKKVTCLTKDNIMKLTDGLFHKVFDEIAKEYPDIEAEHYIVDIGAARLATNPENFDVIVTPNLYGDILSDIAAEISGS 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 233 PGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDnENMHTPDIGGQG 312
Cdd:PRK09222 245 VGLAGSANIGEEYAMFE-AVHGSAPDIAGKNIANPSGLLNAAVMMLVHIGQFDIAELIENAWLKTLE-DGIHTADIYNEG 322
|
....
gi 1039796369 313 TTSQ 316
Cdd:PRK09222 323 VSKK 326
|
|
| Iso_dh |
pfam00180 |
Isocitrate/isopropylmalate dehydrogenase; |
2-321 |
1.75e-88 |
|
Isocitrate/isopropylmalate dehydrogenase;
Pssm-ID: 425507 Cd Length: 346 Bit Score: 269.16 E-value: 1.75e-88
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 2 IPGDGIGPELMLHVKSVFRHACVPV----DFEEVHVSSNA-DEEDI---RNAIMAIRRNRVALKGNIETNHNLPPSHKSR 73
Cdd:pfam00180 5 LPGDGIGPEVMAAALKVLKAALEKAplefEFEERDVGGAAiDETGEplpDETLEACKKADAVLLGAVGGPKWDPAGVRPE 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 74 NNI--LRTSLDLYANVIHCKSLPGVVTRHK------DIDILIVRENTEGEYSSLEHE---SVAGVVESLKIITKAKSLRI 142
Cdd:pfam00180 85 NGLlaLRKELGLFANLRPAKVFPPLGDASPlkneveGVDIVIVRELTGGIYFGIEKGikgSGNEVAVDTKLYSRDEIERI 164
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 143 AEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIV 222
Cdd:pfam00180 165 ARVAFELARKRGRKKVTSVDKANVLKSSDLWRKIVTEVAKAEYPDVELEHQLVDNAAMQLVKNPSQFDVIVTPNLFGDIL 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 223 NNVCAGLVGGPGLVAGANYG-HVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDH-LKLHSYATSIRKAVLASMDN 300
Cdd:pfam00180 245 SDEASMLTGSLGLLPSASLGaNGFGIFE-PVHGSAPDIAGKGIANPIATILSAAMMLRYsLGLEDAADKIEAAVLKVLES 323
|
330 340
....*....|....*....|....
gi 1039796369 301 ENMhTPDIGGQGT---TSQAIQDI 321
Cdd:pfam00180 324 GIR-TGDLAGSATyvsTSEFGEAV 346
|
|
| PRK14025 |
PRK14025 |
multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase; Provisional |
2-325 |
1.54e-80 |
|
multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase; Provisional
Pssm-ID: 184462 Cd Length: 330 Bit Score: 248.12 E-value: 1.54e-80
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 2 IPGDGIGPELMLHVKSVFRhaCVPVDFEEVHVSSnADEEDIR-------NAIMAIRRNRVALKGNIETnhnlppSHKSRN 74
Cdd:PRK14025 7 IEGDGIGKEVVPAALHVLE--ATGLPFEFVYAEA-GDEVFEKtgkalpeETIEAAKEADAVLFGAAGE------TAADVI 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 75 NILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQ--- 151
Cdd:PRK14025 78 VKLRRILDTYANVRPVKSYKGVKCLYPDIDYVIVRENTEGLYKGIEAEIADGVTVATRVITRKASERIFRFAFEMAKrrk 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 152 -ESGRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLV 230
Cdd:PRK14025 158 kMGKEGKVTCAHKANVLKKTDGLFKKTFYEVAKEYPDIKAEDYYVDAMNMYIITRPQTFDVVVTSNLFGDILSDGAAGLV 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 231 GGPGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDnENMHTPDIGG 310
Cdd:PRK14025 238 GGLGLAPSANIGDKYGLFE-PVHGSAPDIAGKGIANPTATILTAVLMLRHLGENEEADKVEKALEEVLA-LGLTTPDLGG 315
|
330
....*....|....*
gi 1039796369 311 QGTTSQAIQDIIRHI 325
Cdd:PRK14025 316 NLSTMEMAEEVAKRV 330
|
|
| LEU3_arch |
TIGR02088 |
isopropylmalate/isohomocitrate dehydrogenases; This model represents a group of archaeal ... |
2-322 |
9.80e-79 |
|
isopropylmalate/isohomocitrate dehydrogenases; This model represents a group of archaeal decarboxylating dehydrogenases which include the leucine biosynthesis enzyme 3-isopropylmalate dehydrogenase (LeuB, LEU3) and the methanogenic cofactor CoB biosynthesis enzyme isohomocitrate dehydrogenase (AksF). Both of these have been characterized in Methanococcus janaschii. Non-methanogenic archaea have only one hit to this model and presumably this is LeuB, although phylogenetic trees cannot establish which gene is which in the methanogens. The AksF gene is capable of acting on isohomocitrate, iso(homo)2-citrate and iso(homo)3-citrate in the successive elongation cycles of coenzyme B (7-mercaptoheptanoyl-threonine phosphate). This family is closely related to both the LeuB genes found in TIGR00169 and the mitochondrial eukaryotic isocitrate dehydratases found in TIGR00175. All of these are included within the broader subfamily model, pfam00180.
Pssm-ID: 273962 Cd Length: 322 Bit Score: 243.52 E-value: 9.80e-79
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 2 IPGDGIGPELMLHVKSVFRHACVPVDFEEVH--------VSSNADEEDIRnaimAIRRNRVALKGNIETNhnLPPSHKSR 73
Cdd:TIGR02088 4 IPGDGIGPEVIEAAIRILNKLGLEIEFIEFEagdealkkYGSALPEDTLE----EIRKADAILFGAVTTP--ANPGYKSV 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 74 NNILRTSLDLYANVIHCKSLPGVVTRH-KDIDILIVRENTEGEYSSLEhESVAGVVESLKIITKAKSLRIAEYAFKLAQE 152
Cdd:TIGR02088 78 IVTLRKELDLYANVRPAKSLPGIPDLYpNGKDIVIVRENTEGLYAGFE-FGFSDRAIAIRVITREGSERIARFAFNLAKE 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 153 SGRKkVTAVHKANIMKLGDGLFLQCCREVAAHYpQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGG 232
Cdd:TIGR02088 157 RNRK-VTCVHKANVLKGTDGLFREVCREIAKRY-GVEYRDMYVDSAAMNLVKDPWRFDVIVTTNMFGDILSDLASALAGS 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 233 PGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMhTPDIGGQG 312
Cdd:TIGR02088 235 LGLAPSANIGDRKALFE-PVHGSAPDIAGKGIANPTAAILSVAMMLDYLGELEKGKLVWEAVEYYIIEGKK-TPDLGGTA 312
|
330
....*....|
gi 1039796369 313 TTSQAIQDII 322
Cdd:TIGR02088 313 KTKEVGDEIA 322
|
|
| ICDH_alpha |
TIGR02924 |
isocitrate dehydrogenase; This family of mainly alphaproteobacterial enzymes is a member of ... |
4-316 |
1.97e-77 |
|
isocitrate dehydrogenase; This family of mainly alphaproteobacterial enzymes is a member of the isocitrate/isopropylmalate dehydrogenase superfamily described by pfam00180. Every member of the seed of this model appears to have a TCA cycle lacking only a determined isocitrate dehydrogenase. The precise identity of the cofactor (NADH -- 1.1.1.41 vs. NADPH -- 1.1.1.42) is unclear. [Energy metabolism, TCA cycle]
Pssm-ID: 274353 [Multi-domain] Cd Length: 473 Bit Score: 244.67 E-value: 1.97e-77
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 4 GDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIM-----AIRRNRVALKGNIETNHNlpPSHKSRNNILR 78
Cdd:TIGR02924 8 GDGIGPEIMEAVLLILKEAEAPIDIETIEIGEKVYKKGWPSGISpssweSIRRTKVLLKAPITTPQG--GGHKSLNVTLR 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 79 TSLDLYANVIHCKSL-PGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKK 157
Cdd:TIGR02924 86 KTLGLYANIRPCVSYhPFIETKSPNLNIVIVRENEEDLYTGIEYRQTPDTYECTKLITRSGSEKICRYAFEYARKHNRKK 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 158 VTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVA 237
Cdd:TIGR02924 166 VTCLTKDNIMKMTDGIFHKIFDKIAAEYPDIESEHYIVDIGMARLATNPENFDVIVTPNLYGDILSDVAAEISGSVGLAG 245
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1039796369 238 GANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDnENMHTPDIGGQGTTSQ 316
Cdd:TIGR02924 246 SANIGEEYAMFE-AVHGSAPDIAGQNIANPSGLLNAAIQMLVHIGQSDIAQLIYNAWLKTLE-DGVHTADIYNEKTSKQ 322
|
|
| PRK00772 |
PRK00772 |
3-isopropylmalate dehydrogenase; Provisional |
2-326 |
9.34e-54 |
|
3-isopropylmalate dehydrogenase; Provisional
Pssm-ID: 234832 Cd Length: 358 Bit Score: 179.91 E-value: 9.34e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 2 IPGDGIGPELM---LHV-KSVFRHACVPVDFEEVHVSSNA-DE--EDIRNAIMAIRRNRVA-LKGNIETNH--NLPPSHK 71
Cdd:PRK00772 8 LPGDGIGPEVMaeaVKVlDAVAEKFGFDFEFEEALVGGAAiDAhgVPLPEETLEACRAADAvLLGAVGGPKwdNLPPDVR 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 72 SRNNIL--RTSLDLYAN---------VIHCKSL-PGVVtrhKDIDILIVRENTEGEY-------SSLEHESVAgvVESLk 132
Cdd:PRK00772 88 PERGLLalRKELGLFANlrpaklypgLADASPLkPEIV---AGLDILIVRELTGGIYfgeprgrEGLGGEERA--FDTM- 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 133 IITKAKSLRIAEYAFKLAQESgRKKVTAVHKANIMKlGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVM 212
Cdd:PRK00772 162 VYTREEIERIARVAFELARKR-RKKVTSVDKANVLE-SSRLWREVVTEVAKEYPDVELSHMYVDNAAMQLVRNPKQFDVI 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 213 VMPNLYGNIVNNVCAGLVGGPGLVAGANYGHV-YAVFETAtrntGKS---IANKNIANPTATLLASCMMLDH-LKLHSYA 287
Cdd:PRK00772 240 VTENLFGDILSDEAAMLTGSLGMLPSASLGESgPGLYEPI----HGSapdIAGKGIANPIATILSAAMMLRYsLGLEEAA 315
|
330 340 350 360
....*....|....*....|....*....|....*....|..
gi 1039796369 288 TSIRKAVLASMDnENMHTPDI---GGQGTTSQAIQDIIRHIR 326
Cdd:PRK00772 316 DAIEAAVEKVLA-QGYRTADIaegGGKVSTSEMGDAILAALA 356
|
|
| PRK03437 |
PRK03437 |
3-isopropylmalate dehydrogenase; Provisional |
1-309 |
1.32e-47 |
|
3-isopropylmalate dehydrogenase; Provisional
Pssm-ID: 179579 Cd Length: 344 Bit Score: 163.56 E-value: 1.32e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 1 MIPGDGIGPELMLHVKSVFRHACVP-VDFEEVH-------------VSSNADEEDIR--NAIM--AIRRNRVAlKGNIET 62
Cdd:PRK03437 9 VIPGDGIGPEVVAEALKVLDAVAAGgPGVETTEydlgarrylrtgeTLPDSVLAELRqhDAILlgAIGDPSVP-SGVLER 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 63 NHNLPpshksrnniLRTSLDLYANVIHCKSLPGVVTRHK---DIDILIVRENTEGEYSS--------LEHEsVAGVVEsl 131
Cdd:PRK03437 88 GLLLK---------LRFALDHYVNLRPSKLYPGVTSPLAgpgDIDFVVVREGTEGPYTGnggalrvgTPHE-VATEVS-- 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 132 kIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDV 211
Cdd:PRK03437 156 -VNTAFGVERVVRDAFERAQKRPRKHLTLVHKTNVLTFAGDLWQRTVDEVAAEYPDVTVDYQHVDAATIFMVTDPSRFDV 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 212 MVMPNLYGNIVNNVCAGLVGGPGLVAGANY---GHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAT 288
Cdd:PRK03437 235 IVTDNLFGDIITDLAAAVTGGIGLAASGNInptGTNPSMFE-PVHGSAPDIAGQGIADPTAAILSVALLLDHLGEEDAAA 313
|
330 340
....*....|....*....|....
gi 1039796369 289 SIRKAV---LASMDNENMHTPDIG 309
Cdd:PRK03437 314 RIEAAVeadLAERGKMGRSTAEVG 337
|
|
| PRK08194 |
PRK08194 |
tartrate dehydrogenase; Provisional |
2-326 |
2.74e-47 |
|
tartrate dehydrogenase; Provisional
Pssm-ID: 181281 Cd Length: 352 Bit Score: 162.97 E-value: 2.74e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 2 IPGDGIGPELMLHVKSVFR-----HACVPVDFEEVHVSSnadEEDIRNAIMAIRRNRVALKgNIET-------NHNLPPS 69
Cdd:PRK08194 9 IPGDGVGKEVVPAAVRVLKavaevHGGLKFEFTEFPWSC---EYYLEHGEMMPEDGLEQLK-QFDAiflgavgNPKLVPD 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 70 HKSRNNIL---RTSLDLYANVIHCKSLPGV---VTRHKDIDILIVRENTEGEYSSL-------EHEsvagVVESLKIITK 136
Cdd:PRK08194 85 HISLWGLLikiRREFEQVINIRPAKQLRGIkspLANPKDFDLLVVRENSEGEYSEVggrihrgEDE----IAIQNAVFTR 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 137 AKSLRIAEYAFKLAQESgRKKVTAVHKAN----IMKLGDGLFlqccREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVM 212
Cdd:PRK08194 161 KGTERAMRYAFELAAKR-RKHVTSATKSNgivhSMPFWDEVF----QEVGKDYPEIETDSQHIDALAAFFVTRPEEFDVI 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 213 VMPNLYGNIVNNVCAGLVGGPGLVAGAN---YGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKlHSYATS 289
Cdd:PRK08194 236 VASNLFGDILTDIGAAIMGSIGIAPAANinvNGKYPSMFE-PVHGSAPDIAGKGIANPIGQIWTAKLMLDHFG-EEELGS 313
|
330 340 350
....*....|....*....|....*....|....*..
gi 1039796369 290 IRKAVLASMDNENMHTPDIGGQGTTSQAIQDIIRHIR 326
Cdd:PRK08194 314 HLLDVIEDVTEDGIKTPDIGGRATTDEVTDEIISRLK 350
|
|
| leuB |
TIGR00169 |
3-isopropylmalate dehydrogenase; Several NAD- or NADP-dependent dehydrogenases, including ... |
1-325 |
1.81e-45 |
|
3-isopropylmalate dehydrogenase; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the dimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates.Among these decarboxylating dehydrogenases of hydroxyacids, overall sequence homology indicates evolutionary history rather than actual substrate or cofactor specifity, which may be toggled experimentally by replacement of just a few amino acids. 3-isopropylmalate dehydrogenase is an NAD-dependent enzyme and should have a sequence resembling HGSAPDI around residue 340. The subtrate binding loop should include a sequence resembling E[KQR]X(0,1)LLXXR around residue 115. Other contacts of importance are known from crystallography but not detailed here.This model will not find all isopropylmalate dehydrogenases; the enzyme from Sulfolobus sp. strain 7 is more similar to mitochondrial NAD-dependent isocitrate dehydrogenases than to other known isopropylmalate dehydrogenases and was omitted to improve the specificity of the model. It scores below the cutoff and below some enzymes known not to be isopropylmalate dehydrogenase. [Amino acid biosynthesis, Pyruvate family]
Pssm-ID: 272939 Cd Length: 346 Bit Score: 157.95 E-value: 1.81e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 1 MIPGDGIGPELM---LHV-KSVFRHACVPVDFEEVHVSSNADE---EDIRNAIMAIRRNRVA-LKGNIETNH--NLPPSH 70
Cdd:TIGR00169 4 VLPGDGIGPEVMaqaLKVlKAVAERFGLKFEFEEHLIGGAAIDatgQPLPEETLKGCKEADAvLLGAVGGPKwdNLPRDQ 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 71 KSRNNIL--RTSLDLYANVIHCKSLPGVVTRH-------KDIDILIVRENTEGEYSSLEHE-SVAGVVESLKIITKAKSL 140
Cdd:TIGR00169 84 RPEQGLLklRKSLDLFANLRPAKVFPGLEDLSplkeeiaKGVDFVVVRELTGGIYFGEPKGrEGEGEAWDTEVYTVPEIE 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 141 RIAEYAFKLAQESgRKKVTAVHKANIMKLGDgLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGN 220
Cdd:TIGR00169 164 RIARVAFEMARKR-RKKVTSVDKANVLESSR-LWRKTVEEIAKEYPDVELEHQYIDNAAMQLVKSPTQFDVVVTSNLFGD 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 221 IVNNVCAGLVGGPGLVAGANYGH-VYAVFETAtRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMD 299
Cdd:TIGR00169 242 ILSDEASVITGSLGMLPSASLGSdGFGLFEPV-HGSAPDIAGKGIANPIAQILSAAMLLRYSFNLEEAADAIEAAVKKVL 320
|
330 340
....*....|....*....|....*.
gi 1039796369 300 NENMHTPDIGGQGTTSQAIQDIIRHI 325
Cdd:TIGR00169 321 AEGYRTPDLGSSATTAVGTAEMGEEL 346
|
|
| PRK06451 |
PRK06451 |
NADP-dependent isocitrate dehydrogenase; |
2-308 |
1.07e-42 |
|
NADP-dependent isocitrate dehydrogenase;
Pssm-ID: 235803 Cd Length: 412 Bit Score: 152.29 E-value: 1.07e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 2 IPGDGIGPELMLHVKSVFRHACV-------PVDFEEVHVSSNADEED----IRNAIMAIRRNRVALKGNIETNhnLPPSH 70
Cdd:PRK06451 29 VEGDGIGPEITHAAMKVINKAVEkaygsdrEIKWVEVLAGDKAEKLTgnrfPKESEELIEKYRVLLKGPLETP--IGKGW 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 71 KSRNNILRTSLDLYANVIHCKSLPGVVTRHKD---IDILIVRENTEGEYSSLEH----------------------ESVA 125
Cdd:PRK06451 107 KSINVAIRLMLDLYANIRPVKYIPGIESPLKNpekIDLIIFRENTDDLYRGIEYpydseeakkirdflrkelgvevEDDT 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 126 GVveSLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVA---------------------AH 184
Cdd:PRK06451 187 GI--GIKLISKFKTQRIARMAIKYAIDHKRKKVTIMHKGNVMKYTEGAFREWAYEVAlkefrdyvvteeevtknyngvPP 264
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 185 YPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFEtATRNTGKSIANKNI 264
Cdd:PRK06451 265 SGKVIINDRIADNMFQQIIIRPDEYDIILAPNVNGDYISDAAGALVGNIGMLGGANIGDTGGMFE-AIHGTAPKYAGKNV 343
|
330 340 350 360
....*....|....*....|....*....|....*....|....
gi 1039796369 265 ANPTATLLASCMMLDHLKLHSYATSIRKAVLASMdNENMHTPDI 308
Cdd:PRK06451 344 ANPTGIIKGGELMLRFMGWDKAADLIDKAIMESI-KQKKVTQDL 386
|
|
| Icd |
COG0538 |
Isocitrate dehydrogenase [Energy production and conversion]; Isocitrate dehydrogenase is part ... |
2-326 |
2.04e-41 |
|
Isocitrate dehydrogenase [Energy production and conversion]; Isocitrate dehydrogenase is part of the Pathway/BioSystem: TCA cycle
Pssm-ID: 440304 Cd Length: 409 Bit Score: 148.72 E-value: 2.04e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 2 IPGDGIGPELMLHVKSVFRHA-------CVPVDFEEVHVS-SNADEEDIR---NAIMAIRRNRVALKGNIETnhnlpP-- 68
Cdd:COG0538 24 IEGDGIGPEITRAIWKVIDAAvekayggKRDIEWKEVDAGeKARDETGDWlpdETAEAIKEYGVGIKGPLTT-----Pvg 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 69 -SHKSRNNILRTSLDLYAN---VIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHES----VAGVVESL--------- 131
Cdd:COG0538 99 gGWRSLNVTIRQILDLYVCrrpVRYFKGVPSPVKHPEKVDIVIFRENTEDIYAGIEWKAgspeALKLIFFLedemgvtvi 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 132 ----------KIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVA-AHYP-------------- 186
Cdd:COG0538 179 rfpedsgigiKPVSDEGTERLVRAAIQYALDNKRKSVTLVHKGNIMKFTEGAFKDWGYEVAeEEFGdkfitegpwekykg 258
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 187 -----QITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYG-HVYAVFE----TATRntg 256
Cdd:COG0538 259 pkpagKIVYKDRIADDMLQQILLRPGEYDVIATKNLNGDYISDALAAQVGGLGIAPGANIGdDGGAEFEathgTAPK--- 335
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1039796369 257 ksIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMhTPD----IGGQG--TTSQAIQDIIRHIR 326
Cdd:COG0538 336 --YAGKDSTNPGSLILSGTMMLRHRGWLEAADLIEKAVEKTIESGKV-TYDlarlMEGATelSTSEFGDAIIENLD 408
|
|
| PRK07006 |
PRK07006 |
isocitrate dehydrogenase; Reviewed |
48-294 |
4.67e-31 |
|
isocitrate dehydrogenase; Reviewed
Pssm-ID: 180792 Cd Length: 409 Bit Score: 120.94 E-value: 4.67e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 48 AIRRNRVALKGNIETnhnlpP---SHKSRNNILRTSLDLYA---NVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEH 121
Cdd:PRK07006 84 LIREYRVAIKGPLTT-----PvggGIRSLNVALRQELDLYVclrPVRYFKGVPSPVKRPEDTDMVIFRENSEDIYAGIEW 158
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 122 EsvAGVVESLKII-------------------------TKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQ 176
Cdd:PRK07006 159 K--AGSAEAKKVIkflqeemgvkkirfpetsgigikpvSEEGTERLVRAAIEYAIDNDRKSVTLVHKGNIMKFTEGAFKD 236
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 177 CCREVA-----------AHYPQ---------ITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLV 236
Cdd:PRK07006 237 WGYQLAeeefgdelidgGPWDKiknpetgkeIIVKDSIADAFLQQILLRPAEYDVIATMNLNGDYISDALAAQVGGIGIA 316
|
250 260 270 280 290
....*....|....*....|....*....|....*....|....*....|....*...
gi 1039796369 237 AGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAV 294
Cdd:PRK07006 317 PGANINDGHAIFE-ATHGTAPKYAGLDKVNPGSVILSAEMMLRHMGWTEAADLIIKSM 373
|
|
| PLN02329 |
PLN02329 |
3-isopropylmalate dehydrogenase |
1-312 |
9.06e-25 |
|
3-isopropylmalate dehydrogenase
Pssm-ID: 215188 Cd Length: 409 Bit Score: 103.61 E-value: 9.06e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 1 MIPGDGIGPELMLHVKSVFRHAC----VPVDFEEVHVSSNA-DEEDI---RNAIMAIRRNRVALKGNI------ETNHNL 66
Cdd:PLN02329 51 LLPGDGIGPEVISVAKNVLQKAGslegLEFDFQEMPVGGAAlDLVGVplpEETFTAAKQSDAILLGAIggykwdKNEKHL 130
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 67 PPSHKSRNniLRTSLDLYANVIHCKSLPGVV---TRHKDI----DILIVRENTEGEYSSLE-----HESVAGVVESLKII 134
Cdd:PLN02329 131 RPEMALFY--LRRDLKVFANLRPATVLPQLVdasTLKKEVaegvDMMIVRELTGGIYFGEPrgitiNENGEEVGVSTEIY 208
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 135 TKAKSLRIAEYAFKLAQESgRKKVTAVHKANIMKlGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVM 214
Cdd:PLN02329 209 AAHEIDRIARVAFETARKR-RGKLCSVDKANVLD-ASILWRKRVTALASEYPDVELSHMYVDNAAMQLIRDPKQFDTIVT 286
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 215 PNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDH-LKLHSYATSIRKA 293
Cdd:PLN02329 287 NNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYgLGEEKAAKRIEDA 366
|
330
....*....|....*....
gi 1039796369 294 VLASMdNENMHTPDIGGQG 312
Cdd:PLN02329 367 VVDAL-NKGFRTGDIYSPG 384
|
|
| PRK07362 |
PRK07362 |
NADP-dependent isocitrate dehydrogenase; |
46-300 |
1.14e-21 |
|
NADP-dependent isocitrate dehydrogenase;
Pssm-ID: 180944 Cd Length: 474 Bit Score: 95.17 E-value: 1.14e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 46 IMAIRRNRVALKGNIETnhnlpP---SHKSRNNILRTSLDLYANVIHCKSLPGVVTRHK---DIDILIVRENTEGEYSSL 119
Cdd:PRK07362 91 LEAIREYGVAIKGPLTT-----PiggGIRSLNVALRQIFDLYSCVRPCRYYAGTPSPHKnpeKLDVIVYRENTEDIYMGI 165
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 120 EHESVAGVVESL----------------------------KIITKAKSLRIAEYAFK--LAQESGRKKVTAVHKANIMKL 169
Cdd:PRK07362 166 EWEAGDEIGDKLikhlneevipaspelgkrqiplgsgigiKPVSKTGSQRHIRRAIEhaLRLPGDKRHVTLVHKGNIMKY 245
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 170 GDGLFLQCCREVAA--------------------HYPQIT-----------FDSM------------------------- 193
Cdd:PRK07362 246 TEGAFRDWGYELATtefrdecvtereswilsnkeKNPNISiednarmiepgYDSLtpekkaaicaevkevldsiwsshgn 325
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039796369 194 ------------IVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFEtATRNTGKSIAN 261
Cdd:PRK07362 326 gkwkekvlvddrIADSIFQQIQTRPQEYSILATLNLNGDYISDAAAAIVGGLGMAPGANIGDNAAIFE-ATHGTAPKHAG 404
|
330 340 350
....*....|....*....|....*....|....*....
gi 1039796369 262 KNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDN 300
Cdd:PRK07362 405 LDRINPGSVILSGVMMLEYLGWQEAADLITKGLSAAIAN 443
|
|
| |
