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Conserved domains on  [gi|578837271|ref|XP_006724329|]
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lactosylceramide 4-alpha-galactosyltransferase isoform X1 [Homo sapiens]

Protein Classification

Gly_transf_sug and Gb3_synth domain-containing protein( domain architecture ID 10376080)

Gly_transf_sug and Gb3_synth domain-containing protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Gb3_synth pfam04572
Alpha 1,4-glycosyltransferase conserved region; The glycosphingolipids (GSL) form part of ...
 222-349 9.42e-61

Alpha 1,4-glycosyltransferase conserved region; The glycosphingolipids (GSL) form part of eukaryotic cell membranes. They consist of a hydrophilic carbohydrate moiety linked to a hydrophobic ceramide tail embedded within the lipid bilayer of the membrane. Lactosylceramide, Gal1,4Glc1Cer (LacCer), is the common synthetic precursor to the majority of GSL found in vertebrates. Alpha 1.4-glycosyltransferases utilize UDP donors and transfer the sugar to a beta-linked acceptor. This region appears to be confined to higher eukaryotes. No function has been yet assigned to this region.


:

Pssm-ID: 428017  Cd Length: 130  Bit Score: 190.92  E-value: 9.42e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578837271  222 AFERRHEFMALCMRDFVDHYNGWIWGHQGPQLLTRVFKKWCSIRSLA--ESRACRGVTTLPPEAFYPIPWQDWKKYFEDI 299
Cdd:pfam04572   1 AFDKGHPFLELCLRDFVENYNGDEWGHNGPGLLTRVLKKFCNTSDLKamERVRCRGFTVLPPEAFYPIPYPNWKRFFEPE 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 578837271  300 NPEELPRLLSATYAVHVWNKKSQGTRFEATSRALLAQLHARYCPTTHEAM 349
Cdd:pfam04572  81 DLEETMKLTKDSYAVHLWNKLSKGEPLKVGSNSLYARLARKHCPRTYAAA 130
Gly_transf_sug super family cl19952
Glycosyltransferase sugar-binding region containing DXD motif; The DXD motif is a short ...
 94-214 1.01e-13

Glycosyltransferase sugar-binding region containing DXD motif; The DXD motif is a short conserved motif found in many families of glycosyltransferases, which add a range of different sugars to other sugars, phosphates and proteins. DXD-containing glycosyltransferases all use nucleoside diphosphate sugars as donors and require divalent cations, usually manganese. The DXD motif is expected to play a carbohydrate binding role in sugar-nucleoside diphosphate and manganese dependent glycosyltransferases.


The actual alignment was detected with superfamily member pfam04488:

Pssm-ID: 418730 [Multi-domain]  Cd Length: 93  Bit Score: 66.20  E-value: 1.01e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578837271   94 FMCSVESAARTHPESHVLVLMKGLpggnaslprhlgisllscfpnvqMLPLDLRELFRDTP-LADWYAAvqgRWepyLLP 172
Cdd:pfam04488   4 VMKAIESLIKLHPDYCYVVLSDDL-----------------------DFALDINFLKSDTPwFLEAYSL---LP---LFI 54

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 578837271  173 VLSDASRIALMWKFGGIYLDTDFIVLKNLrnltNVLGTQSRY 214
Cdd:pfam04488  55 AKSDLLRYAILYKYGGIYLDTDVIPLKSL----NSIGAQERF 92
 
Name Accession Description Interval E-value
Gb3_synth pfam04572
Alpha 1,4-glycosyltransferase conserved region; The glycosphingolipids (GSL) form part of ...
 222-349 9.42e-61

Alpha 1,4-glycosyltransferase conserved region; The glycosphingolipids (GSL) form part of eukaryotic cell membranes. They consist of a hydrophilic carbohydrate moiety linked to a hydrophobic ceramide tail embedded within the lipid bilayer of the membrane. Lactosylceramide, Gal1,4Glc1Cer (LacCer), is the common synthetic precursor to the majority of GSL found in vertebrates. Alpha 1.4-glycosyltransferases utilize UDP donors and transfer the sugar to a beta-linked acceptor. This region appears to be confined to higher eukaryotes. No function has been yet assigned to this region.


Pssm-ID: 428017  Cd Length: 130  Bit Score: 190.92  E-value: 9.42e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578837271  222 AFERRHEFMALCMRDFVDHYNGWIWGHQGPQLLTRVFKKWCSIRSLA--ESRACRGVTTLPPEAFYPIPWQDWKKYFEDI 299
Cdd:pfam04572   1 AFDKGHPFLELCLRDFVENYNGDEWGHNGPGLLTRVLKKFCNTSDLKamERVRCRGFTVLPPEAFYPIPYPNWKRFFEPE 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 578837271  300 NPEELPRLLSATYAVHVWNKKSQGTRFEATSRALLAQLHARYCPTTHEAM 349
Cdd:pfam04572  81 DLEETMKLTKDSYAVHLWNKLSKGEPLKVGSNSLYARLARKHCPRTYAAA 130
Gly_transf_sug pfam04488
Glycosyltransferase sugar-binding region containing DXD motif; The DXD motif is a short ...
 94-214 1.01e-13

Glycosyltransferase sugar-binding region containing DXD motif; The DXD motif is a short conserved motif found in many families of glycosyltransferases, which add a range of different sugars to other sugars, phosphates and proteins. DXD-containing glycosyltransferases all use nucleoside diphosphate sugars as donors and require divalent cations, usually manganese. The DXD motif is expected to play a carbohydrate binding role in sugar-nucleoside diphosphate and manganese dependent glycosyltransferases.


Pssm-ID: 398274 [Multi-domain]  Cd Length: 93  Bit Score: 66.20  E-value: 1.01e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578837271   94 FMCSVESAARTHPESHVLVLMKGLpggnaslprhlgisllscfpnvqMLPLDLRELFRDTP-LADWYAAvqgRWepyLLP 172
Cdd:pfam04488   4 VMKAIESLIKLHPDYCYVVLSDDL-----------------------DFALDINFLKSDTPwFLEAYSL---LP---LFI 54

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 578837271  173 VLSDASRIALMWKFGGIYLDTDFIVLKNLrnltNVLGTQSRY 214
Cdd:pfam04488  55 AKSDLLRYAILYKYGGIYLDTDVIPLKSL----NSIGAQERF 92
OCH1 COG3774
Mannosyltransferase OCH1 or related enzyme [Cell wall/membrane/envelope biogenesis];
175-234 8.85e-07

Mannosyltransferase OCH1 or related enzyme [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 442988 [Multi-domain]  Cd Length: 136  Bit Score: 47.58  E-value: 8.85e-07
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 578837271 175 SDASRIALMWKFGGIYLDTDFIVLKNLRNLTN----VLGTQ--SRYVLNGAFLAFERRHEFMALCM 234
Cdd:COG3774   70 ADLFRLLLLYKYGGIYLDIDVECLKPLDPLLDgddlFLGYEdpPPGIISNGFIAAEPGHPFLKKAL 135
 
Name Accession Description Interval E-value
Gb3_synth pfam04572
Alpha 1,4-glycosyltransferase conserved region; The glycosphingolipids (GSL) form part of ...
 222-349 9.42e-61

Alpha 1,4-glycosyltransferase conserved region; The glycosphingolipids (GSL) form part of eukaryotic cell membranes. They consist of a hydrophilic carbohydrate moiety linked to a hydrophobic ceramide tail embedded within the lipid bilayer of the membrane. Lactosylceramide, Gal1,4Glc1Cer (LacCer), is the common synthetic precursor to the majority of GSL found in vertebrates. Alpha 1.4-glycosyltransferases utilize UDP donors and transfer the sugar to a beta-linked acceptor. This region appears to be confined to higher eukaryotes. No function has been yet assigned to this region.


Pssm-ID: 428017  Cd Length: 130  Bit Score: 190.92  E-value: 9.42e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578837271  222 AFERRHEFMALCMRDFVDHYNGWIWGHQGPQLLTRVFKKWCSIRSLA--ESRACRGVTTLPPEAFYPIPWQDWKKYFEDI 299
Cdd:pfam04572   1 AFDKGHPFLELCLRDFVENYNGDEWGHNGPGLLTRVLKKFCNTSDLKamERVRCRGFTVLPPEAFYPIPYPNWKRFFEPE 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 578837271  300 NPEELPRLLSATYAVHVWNKKSQGTRFEATSRALLAQLHARYCPTTHEAM 349
Cdd:pfam04572  81 DLEETMKLTKDSYAVHLWNKLSKGEPLKVGSNSLYARLARKHCPRTYAAA 130
Gly_transf_sug pfam04488
Glycosyltransferase sugar-binding region containing DXD motif; The DXD motif is a short ...
 94-214 1.01e-13

Glycosyltransferase sugar-binding region containing DXD motif; The DXD motif is a short conserved motif found in many families of glycosyltransferases, which add a range of different sugars to other sugars, phosphates and proteins. DXD-containing glycosyltransferases all use nucleoside diphosphate sugars as donors and require divalent cations, usually manganese. The DXD motif is expected to play a carbohydrate binding role in sugar-nucleoside diphosphate and manganese dependent glycosyltransferases.


Pssm-ID: 398274 [Multi-domain]  Cd Length: 93  Bit Score: 66.20  E-value: 1.01e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578837271   94 FMCSVESAARTHPESHVLVLMKGLpggnaslprhlgisllscfpnvqMLPLDLRELFRDTP-LADWYAAvqgRWepyLLP 172
Cdd:pfam04488   4 VMKAIESLIKLHPDYCYVVLSDDL-----------------------DFALDINFLKSDTPwFLEAYSL---LP---LFI 54

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 578837271  173 VLSDASRIALMWKFGGIYLDTDFIVLKNLrnltNVLGTQSRY 214
Cdd:pfam04488  55 AKSDLLRYAILYKYGGIYLDTDVIPLKSL----NSIGAQERF 92
OCH1 COG3774
Mannosyltransferase OCH1 or related enzyme [Cell wall/membrane/envelope biogenesis];
175-234 8.85e-07

Mannosyltransferase OCH1 or related enzyme [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 442988 [Multi-domain]  Cd Length: 136  Bit Score: 47.58  E-value: 8.85e-07
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 578837271 175 SDASRIALMWKFGGIYLDTDFIVLKNLRNLTN----VLGTQ--SRYVLNGAFLAFERRHEFMALCM 234
Cdd:COG3774   70 ADLFRLLLLYKYGGIYLDIDVECLKPLDPLLDgddlFLGYEdpPPGIISNGFIAAEPGHPFLKKAL 135
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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