Barrier to autointegration factor; The BAF protein has a SAM-domain-like bundle of orthogonally packed alpha-hairpins - one classic and one pseudo helix-hairpin-helix motif. The protein is involved in the prevention of retroviral DNA integration.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46402307     4 MSPRLRAFLSEPIGEKDVAWVDGISRELAINLVTKGFNKAYVLLGQFLLMHKNEAEFQRWIICCCGATECEARQSSTCLK 83
Cdd:smart01023  2 TSQKHRNFVGEPMGEKPVTALAGIGEVLGGRLETKGFDKAYVVLGQFLLLKKDEELFKEWLKDTCGANAKQARDCYNCLR 81

                  ....*.
gi 46402307    84 EWCSCF 89
Cdd:smart01023 82 EWCDSF 87

Barrier to autointegration factor; Barrier-to-autointegration factor (BAF) is an essential protein that is highly conserved in metazoan evolution, and which may act as a DNA-bridging protein. BAF binds directly to double-stranded DNA, to transcription activators, and to inner nuclear membrane proteins, including lamin A filament proteins that anchor nuclear-pore complexes in place, and nuclear LEM-domain proteins that bind to laminins filaments and chromatin. New findings suggest that BAF has structural roles in nuclear assembly and chromatin organization, represses gene expression and might interlink chromatin structure, nuclear architecture and gene regulation in metazoans. BAF can be exploited by retroviruses to act as a host component of pre-integration complexes, which promote the integration of the retroviral DNA into the host chromosome by preventing autointegration of retroviral DNA. BAF might contribute to the assembly or activity of retroviral pre-integration complexes through direct binding to the retroviral proteins p55 Gag and matrix, as well as to DNA.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46402307     4 MSPRLRAFLSEPIGEKDVAWVDGISRELAINLVTKGFNKAYVLLGQFLLMHKNEAEFQRWIICCCGATECEARQSSTCLK 83
Cdd:smart01023  2 TSQKHRNFVGEPMGEKPVTALAGIGEVLGGRLETKGFDKAYVVLGQFLLLKKDEELFKEWLKDTCGANAKQARDCYNCLR 81

                  ....*.
gi 46402307    84 EWCSCF 89
Cdd:smart01023 82 EWCDSF 87

Barrier to autointegration factor; Barrier-to-autointegration factor (BAF) is an essential protein that is highly conserved in metazoan evolution, and which may act as a DNA-bridging protein. BAF binds directly to double-stranded DNA, to transcription activators, and to inner nuclear membrane proteins, including lamin A filament proteins that anchor nuclear-pore complexes in place, and nuclear LEM-domain proteins that bind to laminins filaments and chromatin. New findings suggest that BAF has structural roles in nuclear assembly and chromatin organization, represses gene expression and might interlink chromatin structure, nuclear architecture and gene regulation in metazoans. BAF can be exploited by retroviruses to act as a host component of pre-integration complexes, which promote the integration of the retroviral DNA into the host chromosome by preventing autointegration of retroviral DNA. BAF might contribute to the assembly or activity of retroviral pre-integration complexes through direct binding to the retroviral proteins p55 Gag and matrix, as well as to DNA.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46402307     4 MSPRLRAFLSEPIGEKDVAWVDGISRELAINLVTKGFNKAYVLLGQFLLMHKNEAEFQRWIICCCGATECEARQSSTCLK 83
Cdd:smart01023  2 TSQKHRNFVGEPMGEKPVTALAGIGEVLGGRLETKGFDKAYVVLGQFLLLKKDEELFKEWLKDTCGANAKQARDCYNCLR 81

                  ....*.
gi 46402307    84 EWCSCF 89
Cdd:smart01023 82 EWCDSF 87