calponin homology (CH) domain found in calponin-3; Calponin-3 (CNN3), also called acidic isoform calponin, is an F-actin-binding protein that is expressed in the brain and has been shown to control dendritic spine morphology, density, and plasticity by regulating actin cytoskeletal reorganization and dynamics. It contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502923   23 KYDHQAEEDLRNWIEEVTGMSIGPNFQLGLKDGIILCELINKLQPGSVKKVNESSLNWPQLENIGNFIKAIQAYGMKPHD 102
Cdd:cd21284   1 KYDPQKEEELRNWIEEVTGMSIGENFQKGLKDGVILCELINKLQPGSIRKINESKLNWHQLENIGNFIKAIQAYGMKPHD 80

                        90       100       110
                ....*....|....*....|....*....|.
gi 4502923  103 IFEANDLFENGNMTQVQTTLVALAGLAKTKG 133
Cdd:cd21284  81 IFEANDLFENGNMTQVQTTLLALAGLAKTKG 111

Calponin family repeat;

                          10        20
                  ....*....|....*....|....*
gi 4502923    243 ISLQMGTNKVASQKGMSVYGLGRQV 267
Cdd:pfam00402   1 IGLQMGTNKGASQSGMTAFGAVRHI 25

calponin homology (CH) domain found in calponin-3; Calponin-3 (CNN3), also called acidic isoform calponin, is an F-actin-binding protein that is expressed in the brain and has been shown to control dendritic spine morphology, density, and plasticity by regulating actin cytoskeletal reorganization and dynamics. It contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502923   23 KYDHQAEEDLRNWIEEVTGMSIGPNFQLGLKDGIILCELINKLQPGSVKKVNESSLNWPQLENIGNFIKAIQAYGMKPHD 102
Cdd:cd21284   1 KYDPQKEEELRNWIEEVTGMSIGENFQKGLKDGVILCELINKLQPGSIRKINESKLNWHQLENIGNFIKAIQAYGMKPHD 80

                        90       100       110
                ....*....|....*....|....*....|.
gi 4502923  103 IFEANDLFENGNMTQVQTTLVALAGLAKTKG 133
Cdd:cd21284  81 IFEANDLFENGNMTQVQTTLLALAGLAKTKG 111

Calponin homology (CH) domain; The CH domain is found in both cytoskeletal proteins and signal transduction proteins. The CH domain is involved in actin binding in some members of the family. However in calponins there is evidence that the CH domain is not involved in its actin binding activity. Most member proteins have from two to four copies of the CH domain, however some proteins such as calponin have only a single copy.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502923     26 HQAEEDLRNWIEEVTGMSIGP----NFQLGLKDGIILCELINKLQPGSVKKVNESSLNWPQLENIGNFIK-AIQAYGMKP 100
Cdd:pfam00307   1 LELEKELLRWINSHLAEYGPGvrvtNFTTDLRDGLALCALLNKLAPGLVDKKKLNKSEFDKLENINLALDvAEKKLGVPK 80

                          90       100       110
                  ....*....|....*....|....*....|.
gi 4502923    101 HDIfEANDLFENGNMtQVQTTLVALAGLAKT 131
Cdd:pfam00307  81 VLI-EPEDLVEGDNK-SVLTYLASLFRRFQA 109

Calponin family repeat;

                          10        20
                  ....*....|....*....|....*
gi 4502923    164 IGLQMGTNKCASQAGMTAYGTRRHL 188
Cdd:pfam00402   1 IGLQMGTNKGASQSGMTAFGAVRHI 25

Calponin family repeat;

                          10        20
                  ....*....|....*....|....*
gi 4502923    204 ISLQMGTNKGASQAGMLAPGTRRDI 228
Cdd:pfam00402   1 IGLQMGTNKGASQSGMTAFGAVRHI 25

calponin homology (CH) domain found in calponin-3; Calponin-3 (CNN3), also called acidic isoform calponin, is an F-actin-binding protein that is expressed in the brain and has been shown to control dendritic spine morphology, density, and plasticity by regulating actin cytoskeletal reorganization and dynamics. It contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502923   23 KYDHQAEEDLRNWIEEVTGMSIGPNFQLGLKDGIILCELINKLQPGSVKKVNESSLNWPQLENIGNFIKAIQAYGMKPHD 102
Cdd:cd21284   1 KYDPQKEEELRNWIEEVTGMSIGENFQKGLKDGVILCELINKLQPGSIRKINESKLNWHQLENIGNFIKAIQAYGMKPHD 80

                        90       100       110
                ....*....|....*....|....*....|.
gi 4502923  103 IFEANDLFENGNMTQVQTTLVALAGLAKTKG 133
Cdd:cd21284  81 IFEANDLFENGNMTQVQTTLLALAGLAKTKG 111

calponin homology (CH) domain found in calponin-2; Calponin-2 (CNN2), also called neutral calponin, or smooth muscle calponin H2, is an actin cytoskeleton-associated regulatory protein that inhibits the activity of myosin-ATPase and cytoskeleton dynamics. It contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502923   25 DHQAEEDLRNWIEEVTGMSIGPNFQLGLKDGIILCELINKLQPGSVKKVNESSLNWPQLENIGNFIKAIQAYGMKPHDIF 104
Cdd:cd21283   1 DPQKEAELRTWIEGLTGRSIGPDFQKGLKDGVILCELMNKLQPGSVPKINRSMQNWHQLENLSNFIKAMVSYGMKPVDLF 80

                        90       100
                ....*....|....*....|....*....
gi 4502923  105 EANDLFENGNMTQVQTTLVALAGLAKTKG 133
Cdd:cd21283  81 EANDLFESGNMTQVQVSLLALAGMAKTKG 109

calponin homology (CH) domain found in Drosophila melanogaster muscle-specific protein 20 (dMP20) and similar domains; This subfamily contains Drosophila melanogaster muscle-specific protein 20 (dMP20), Echinococcus granulosus myophilin, Dictyostelium discoideum Rac guanine nucleotide exchange factor B (also called Trix), and similar proteins. dMP20 is present only in the synchronous muscles of D. melanogaster. It may be involved in the system linking the nerve impulse with the contraction or the relaxation process. Trix is involved in the regulation of the late steps of the endocytic pathway. dMP20 contains a single copy of the CH domain, while Trix (triple CH-domain array exchange factor) contains three, two type 3 CH domains which are included in this model, and one type 1 CH domain that is not included in this subfamily, but is part of the superfamily. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502923   23 KYDHQAEEDLRNWIEEVTGMSI--GPNFQLGLKDGIILCELINKLQPGSVKKVNESSLNWPQLENIGNFIKAIQAYGMKP 100
Cdd:cd21207   1 KRDPALEAEALDWIEAVTGEKLddGKDYEDVLKDGVILCKLINILKPGSVKKINTSKMAFKLMENIENFLTACKGYGVPK 80

                        90       100
                ....*....|....*....|....*..
gi 4502923  101 HDIFEANDLFENGNMTQVQTTLVALAG 127
Cdd:cd21207  81 TDLFQTVDLYEKKNIPQVTNCLFALGR 107

calponin homology (CH) domain superfamily; CH domains are actin filament (F-actin) binding motifs, which may be present as a single copy or in tandem repeats (which increase binding affinity). They either function as autonomous actin binding motifs or serve a regulatory function. CH domains are found in cytoskeletal and signal transduction proteins, including actin-binding proteins like spectrin, alpha-actinin, dystrophin, utrophin, and fimbrin, as well as proteins essential for regulation of cell shape (cortexillins), and signaling proteins (Vav).

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502923   29 EEDLRNWIEEVTGMSIGP---NFQLGLKDGIILCELINKLQPGSVKKVNESSLN-WPQLENIGNFIKAIQAYGMKPHDIF 104
Cdd:cd00014   1 EEELLKWINEVLGEELPVsitDLFESLRDGVLLCKLINKLSPGSIPKINKKPKSpFKKRENINLFLNACKKLGLPELDLF 80

                        90       100
                ....*....|....*....|..
gi 4502923  105 EANDLFENGNMTQVQTTLVALA 126
Cdd:cd00014  81 EPEDLYEKGNLKKVLGTLWALA 102

calponin homology (CH) domain found in the transgelin family; The transgelin (TAGLN) family includes transgelin, transgelin-2 and transgelin-3. Transgelin, also called 22 kDa actin-binding protein, protein WS3-10, or smooth muscle protein 22-alpha (SM22-alpha), acts as an actin cross-linking/gelling protein that may be involved in calcium interactions and in regulating contractile properties of the cell. Transgelin-2, also called epididymis tissue protein Li 7e, or SM22-alpha homolog, acts as an actin-binding protein that induces actin gelation and regulates actin cytoskeleton. It may participate in the development and progression of multiple cancers. Transgelin-3, also called neuronal protein 22 (NP22), or neuronal protein NP25, may have a role in alcohol-related adaptations and may mediate regulatory signal transduction pathways in neurons. Members of this family contain a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502923   25 DHQAEEDLRNWIEEVTGMSIGP------NFQLGLKDGIILCELINKLQP---GSVKKVNESSLNWPQLENIGNFIKAIQA 95
Cdd:cd21209   1 DPELESRLVEWIVAQCGSDVGRpdpgrlGFQKWLKDGTVLCKLINSLYPegsKPVKKIQSSKMAFKQMEQISQFLKAAED 80

                        90       100       110
                ....*....|....*....|....*....|....*..
gi 4502923   96 YGMKPHDIFEANDLFENGNMTQVQTTLVALAGLAKTK 132
Cdd:cd21209  81 YGVRTTDIFQTVDLWEGKDMAAVQRTLMALGSLAVTK 117

Calponin homology (CH) domain; The CH domain is found in both cytoskeletal proteins and signal transduction proteins. The CH domain is involved in actin binding in some members of the family. However in calponins there is evidence that the CH domain is not involved in its actin binding activity. Most member proteins have from two to four copies of the CH domain, however some proteins such as calponin have only a single copy.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502923     26 HQAEEDLRNWIEEVTGMSIGP----NFQLGLKDGIILCELINKLQPGSVKKVNESSLNWPQLENIGNFIK-AIQAYGMKP 100
Cdd:pfam00307   1 LELEKELLRWINSHLAEYGPGvrvtNFTTDLRDGLALCALLNKLAPGLVDKKKLNKSEFDKLENINLALDvAEKKLGVPK 80

                          90       100       110
                  ....*....|....*....|....*....|.
gi 4502923    101 HDIfEANDLFENGNMtQVQTTLVALAGLAKT 131
Cdd:pfam00307  81 VLI-EPEDLVEGDNK-SVLTYLASLFRRFQA 109

calponin homology (CH) domain found in transgelin-3; Transgelin-3, also called neuronal protein 22 (NP22), or neuronal protein NP25, may have a role in alcohol-related adaptations and may mediate regulatory signal transduction pathways in neurons. It contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502923   25 DHQAEEDLRNWIEEVTGMSIG------PNFQLGLKDGIILCELINKLQPGS---VKKVNESSLNWPQLENIGNFIKAIQA 95
Cdd:cd21281   1 DPDLESRLVDWIIIQCGGDIErpqpgrQNFQKWLMDGTILCRLINSLYPPGkepIKKISETKMAFKQMEKISQFLQAAEA 80

                        90       100       110
                ....*....|....*....|....*....|....*..
gi 4502923   96 YGMKPHDIFEANDLFENGNMTQVQTTLVALAGLAKTK 132
Cdd:cd21281  81 YGVITTDIFQTVDLWEGKDMAAVQRTLMALGSVAVTK 117

calponin homology (CH) domain found in transgelin; Transgelin, also called 22 kDa actin-binding protein, protein WS3-10, or smooth muscle protein 22-alpha (SM22-alpha), acts as an actin cross-linking/gelling protein that may be involved in calcium interactions and in regulating contractile properties of the cell. It may also contribute to replicative senescence. Transgelin contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502923   25 DHQAEEDLRNWIEEVTGMSIG-PN-----FQLGLKDGIILCELINKLQPGSVKKV----NESSLNWPQLENIGNFIKAIQ 94
Cdd:cd21279   1 DEELEERLVEWIVVQCGPDVGrPDrgrlgFQVWLKNGVVLSKLVNSLYPDGSKPVkipdNPPSMVFKQMEQVAQFLKAAE 80

                        90       100       110
                ....*....|....*....|....*....|....*...
gi 4502923   95 AYGMKPHDIFEANDLFENGNMTQVQTTLVALAGLAKTK 132
Cdd:cd21279  81 DYGVVKTDMFQTVDLYEGKDMAAVQRTLVALGSLAVTK 118

calponin homology (CH) domain found in LIM domain only protein 7; LIM domain only protein 7 (LMO-7), also called F-box only protein 20, or LOMP, is a transcription regulator for expression of many Emery-Dreifuss muscular dystrophy (EDMD)-relevant genes. It binds to alpha-actinin and AF6/afadin at adherens junctions for epithelial cell-cell adhesion. It contains a single copy of the CH domain at the N-terminus. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 4502923   33 RNWIEEVTGMSIG-PNFQLGLKDGIILCELINKLQPGSVKKVNESSLNWPQLENIGNFIKAIQAYGMKPHDIFEANDL 109
Cdd:cd21277   6 QRWIEAVTGKNFGnKDFRSALENGVLLCDLINKIKPGIIKKINRLSTPIAGLDNINVFLKACEKLGLKEAQLFHPGDL 83

calponin homology (CH) domain found in the IQ motif containing GTPase activating protein family; Members of the IQ motif containing GTPase activating protein (IQGAP) family are associated with the Ras GTP-binding protein and act as essential regulators of cytoskeletal function. There are three known IQGAP family members: IQGAP1, IQGAP2, and IQGAP3. They are multi-domain molecules having a calponin-homology (CH) domain which binds F-actin, IQGAP-specific repeats, a single WW domain, four IQ motifs that mediate interactions with calmodulin, and a RasGAP related domain that binds active Rho family GTPases. IQGAP1 negatively regulates Ras family GTPases by stimulating their intrinsic GTPase activity. It lacks GAP activity. Both IQGAP1 and IQGAP2 specifically bind to Cdc42 and Rac1, but not to RhoA. Despite similarities to part of the sequence of RasGAP, neither IQGAP1 nor IQGAP2 interacts with Ras. IQGAP3 regulates the organization of the cytoskeleton under the regulation of Rac1 and Cdc42 in neuronal cells. The depletion of IQGAP3 is shown to impair neurite or axon outgrowth in neuronal cells with disorganized cytoskeleton.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502923   30 EDLRNWIEEVTGMSIGP--NFQLGLKDGIILCELINKLQPGSV--KKVNESSLNWPQLENIGNFIKAIQAYGMKPHDIFE 105
Cdd:cd21206  11 EEAKQWIEACLNEELPPttEFEEELRNGVVLAKLANKFAPKLVplKKIYDVGLQFRHTDNINHFLRALKKIGLPKIFHFE 90

                        90       100
                ....*....|....*....|....*...
gi 4502923  106 ANDLFENGNMTQVQTTLVALAGLAKTKG 133
Cdd:cd21206  91 TTDLYEKKNIPKVIYCLHALSLLLFKLG 118

calponin homology (CH) domain found in the leucine-rich repeat and calponin homology domain-containing protein family; The leucine-rich repeat and calponin homology domain-containing protein (LRCH) family includes LRCH1-4. LRCH1, also called calponin homology domain-containing protein 1, or neuronal protein 81 (NP81), acts as a negative regulator of GTPase Cdc42 by sequestering Cdc42-guanine exchange factor DOCK8. LRCH2 may play a role in the organization of the cytoskeleton. LRCH3 is part of the DISP complex and may regulate the association of septins with actin and thereby regulate the actin cytoskeleton. LRCH4, also called leucine-rich repeat neuronal protein 4, or leucine-rich neuronal protein, acts as a novel Toll-like receptor (TLR) accessory protein that regulates the innate immune response. Members of this family contain a single copy of the CH domain at the C-terminus. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502923   30 EDLRNWIEEVTGMSIGPNFQLGLKDGIILCELINKLQPGSVKKVNESSLNWPQL------ENIGNFIKAIQAYGMKPHDI 103
Cdd:cd21205   4 EQLRKSIESRLKVTLPDDLGEALMDGVVLCHLANHVRPRSVPSIHVPSPAVPKLsmakcrRNVENFLEACRKLGVPEERL 83

                        90       100
                ....*....|....*....|..
gi 4502923  104 FEANDLFENGNMTQVQTTLVAL 125
Cdd:cd21205  84 CSPGDILEEKGLVRVAVTVQAL 105

calponin homology (CH) domain found in beta-Pak Interactive eXchange factor; Beta-Pak Interactive eXchange factor (beta-PIX), also called PAK-interacting exchange factor beta, Rho guanine nucleotide exchange factor 7 (ARHGEF7), p85, or Cool (Cloned out of Library)-1, activates small GTPases by exchanging bound GDP for free GTP. It acts as a GEF for both Cdc42 and Rac1, and plays important roles in regulating neuroendocrine exocytosis, focal adhesion maturation, cell migration, synaptic vesicle localization, and insulin secretion. Beta-PIX contains a single copy of the CH domain at its N-terminus. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 4502923   48 FQLGLKDGIILCELINKLQPGSVKKV-NESSLNWPQLENIGNFIKAIQAYGMkphDIFEANDLFENGNMTQVQTTLVAL 125
Cdd:cd21266  29 LQASLKDGVVLCRLLERLLPGSIDKVyPEPRTESECLSNIREFLRGCGALRL---ETFDANDLYQGQNFNKVLSSLVAL 104

Calponin family repeat;

                          10        20
                  ....*....|....*....|....*
gi 4502923    164 IGLQMGTNKCASQAGMTAYGTRRHL 188
Cdd:pfam00402   1 IGLQMGTNKGASQSGMTAFGAVRHI 25

calponin homology (CH) domain found in VAV3 protein and similar proteins; VAV3 is ubiquitously expressed and functions as a phosphorylation-dependent guanine nucleotide exchange factor (GEF) for RhoA, RhoG, and Rac1. Its function has been implicated in the hematopoietic, bone, cerebellar, and cardiovascular systems. VAV3 is essential in axon guidance in neurons that control blood pressure and respiration. It is overexpressed in prostate cancer cells and it plays a role in regulating androgen receptor transcriptional activity. VAV proteins contain several domains that enable their function: N-terminal calponin homology (CH), acidic, RhoGEF (also called Dbl-homologous or DH), Pleckstrin Homology (PH), C1 (zinc finger), SH2, and two SH3 domains. The model corresponds to CH domain, an actin-binding domain which is present as a single copy in VAV3 protein.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502923   52 LKDGIILCELINKLQPGSVkKVNESSLNwPQ------LENIGNFIKA-IQAYGMKPHDIFEANDLFENGNMTQVQTTLVA 124
Cdd:cd21264  36 LRDGVLLCQLLNNLRPHSI-NLKEINLR-PQmsqflcLKNIRTFLSAcCETFGMRKSELFEAFDLFDVRDFGKVIETLSK 113

                ..
gi 4502923  125 LA 126
Cdd:cd21264 114 LS 115

calponin homology (CH) domain found in VAV1 protein; VAV1 is expressed predominantly in the hematopoietic system and it plays an important role in the development and activation of B and T cells. It is activated by tyrosine phosphorylation to function as a guanine nucleotide exchange factor (GEF) for Rho GTPases following cell surface receptor activation, triggering various effects such as cytoskeletal reorganization, transcription regulation, cell cycle progression, and calcium mobilization. It also serves as a scaffold protein and has been shown to interact with Ku70, Socs1, Janus kinase 2, SIAH2, S100B, Abl gene, ZAP-70, SLP76, and Syk, among others. VAV proteins contain several domains that enable their function: N-terminal calponin homology (CH), acidic, RhoGEF (also called Dbl-homologous or DH), Pleckstrin Homology (PH), C1 (zinc finger), SH2, and two SH3 domains. This model corresponds to the CH domain, an actin-binding domain which is present as a single copy in VAV1 protein.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502923   52 LKDGIILCELINKLQPGSVkKVNESSLNwPQ------LENIGNFIKAIQ-AYGMKPHDIFEANDLFENGNMTQVQTTLVA 124
Cdd:cd21262  36 LRDGVLLCQLLNNLLPHAV-NLREINLR-PQmsqflcLKNIRTFLSTCCeKFGLRKSELFEAFDLFDVRDFGKVIDTLSI 113

                ..
gi 4502923  125 LA 126
Cdd:cd21262 114 LS 115

Calponin family repeat;

                          10        20
                  ....*....|....*....|....*
gi 4502923    204 ISLQMGTNKGASQAGMLAPGTRRDI 228
Cdd:pfam00402   1 IGLQMGTNKGASQSGMTAFGAVRHI 25

first calponin homology (CH) domain found in the plastin/fimbrin family; This family includes plastin and fimbrin. Plastin has three isoforms, plastin-1, -2, and -3, which are all actin-bundling proteins. Plastin-1, also called intestine-specific plastin, or I-plastin, is an actin-bundling protein in the absence of calcium. Plastin-2, also called L-plastin, LC64P, or lymphocyte cytosolic protein 1 (LCP-1), plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. It modulates the cell surface expression of IL2RA/CD25 and CD69. Plastin-3, also called T-plastin, is found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. It may play a role in the regulation of bone development. Fimbrin has been found in plants and fungi. Arabidopsis thaliana fimbrin (AtFIM) includes fimbrin-1, -2, -3, -4, and -5; they cross-link actin filaments (F-actin) in a calcium independent manner. They stabilize and prevent F-actin depolymerization mediated by profilin. They act as key regulators of actin cytoarchitecture, probably involved in cell cycle, cell division, cell elongation and cytoplasmic tractus. AtFIM5 is an actin bundling factor that is required for pollen germination and pollen tube growth. Fungal fimbrin binds to actin, and functionally associates with actin structures involved in the development and maintenance of cell polarity. Members of this family contain four copies of the CH domain. This model corresponds to the first CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 4502923   52 LKDGIILCELINKLQPGSvkkVNESSLN-------WPQLENIGNFIKAIQAYGMK-----PHDIFEAN 107
Cdd:cd21217  37 LRDGVLLCKLINKIVPGT---IDERKLNkkkpkniFEATENLNLALNAAKKIGCKvvnigPQDILDGN 101

calponin homology (CH) domain found in leucine-rich repeat and calponin homology domain-containing protein 2; Leucine-rich repeat and calponin homology domain-containing protein 2 (LRCH2) may play a role in the organization of the cytoskeleton. It contains a single copy of the CH domain at the C-terminus. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502923   30 EDLRNWIEEVTGMSIGPNFQLGLKDGIILCELINKLQPGSVKKVNESSLNWPQL------ENIGNFIKAIQAYGMK---- 99
Cdd:cd21271   8 EQLRENIESRLKVILPEDLGAALMDGVVLCHLANHIRPRSVGSIHVPSPAVPKLsmakcrRNVENFLDACRKLGVPedkl 87

                        90       100
                ....*....|....*....|....*...
gi 4502923  100 --PHDIFEandlfENGnMTQVQTTLVAL 125
Cdd:cd21271  88 clPHHILE-----EKG-LVKVSVTVQAL 109

calponin homology (CH) domain found in leucine-rich repeat and calponin homology domain-containing protein 4; Leucine-rich repeat and calponin homology domain-containing protein 4 (LRCH4), also called leucine-rich repeat neuronal protein 4, or leucine-rich neuronal protein, acts as a novel Toll-like receptor (TLR) accessory protein that regulates the innate immune response. LRCH4 contains a single copy of the CH domain at the C-terminus. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502923   32 LRNWIEEVTGMSIGPNFQLGLKDGIILCELINKLQPGSVKKVNESSLNWPQL------ENIGNFIKAIQAYGMKPHDIFE 105
Cdd:cd21273   9 LRKTLESRLKVTLPEDLAEALSNGAVLCQLANQLRPRSVSIIHVPSPAVPKLskakcrKNVENFIEACRKMGVPEVDLCS 88

                        90       100
                ....*....|....*....|.
gi 4502923  106 ANDLFENGNMTQVQTTLVALA 126
Cdd:cd21273  89 PSDVLLQGPAAVLRTVLALLA 109

third calponin homology (CH) domain found in the plastin/fimbrin family; This family includes plastin and fimbrin. Plastin has three isoforms, plastin-1, -2, and -3. Plastin-1, also called intestine-specific plastin, or I-plastin, is an actin-bundling protein in the absence of calcium. Plastin-2, also called L-plastin, or LC64P, or lymphocyte cytosolic protein 1 (LCP-1), is an actin-binding protein that plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. It modulates the cell surface expression of IL2RA/CD25 and CD69. Plastin-3, also called T-plastin, is an actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. It may play a role in the regulation of bone development. Fimbrin has been found in plants and fungi. Arabidopsis thaliana fimbrin (AtFIM) includes fimbrin-1, -2, -3, -4, and -5, which cross-link actin filaments (F-actin) in a calcium independent manner. They stabilize and prevent F-actin depolymerization mediated by profilin. They act as key regulators of actin cytoarchitecture, probably involved in cell cycle, cell division, cell elongation and cytoplasmic tractus. AtFIM5 is an actin bundling factor that is required for pollen germination and pollen tube growth. Fungal fimbrin binds to actin, and functionally associates with actin structures involved in the development and maintenance of cell polarity. Members of this family contain four copies of the CH domain. This model corresponds to the third CH domain. CH domains are actin filament (F-actin) binding motifs.

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                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4502923   27 QAEEDLRNWIEEVTGMSIGPNFQLGLKDGIILCELINKLQPGSV--KKVNESSLN--WPQLENIGNFIKAIQAY-----G 97
Cdd:cd21219   4 REERAFRMWLNSLGLDPLINNLYEDLRDGLVLLQVLDKIQPGCVnwKKVNKPKPLnkFKKVENCNYAVDLAKKLgfslvG 83

                        90
                ....*....|
gi 4502923   98 MKPHDIFEAN 107
Cdd:cd21219  84 IGGKDIADGN 93