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Conserved domains on  [gi|954170003|ref|NP_001304148|]
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myosin light polypeptide 6 isoform b [Mus musculus]

Protein Classification

EF-hand domain-containing protein( domain architecture ID 1000080)

EF-hand (EFh) domain-containing protein may be involved in binding intracellular calcium and in calcium signal transduction

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PTZ00184 super family cl33172
calmodulin; Provisional
 1-115 6.84e-27

calmodulin; Provisional


The actual alignment was detected with superfamily member PTZ00184:

Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 96.75  E-value: 6.84e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 954170003   1 MRALGQNPTNAEVLKVLGNPKSDemNVKVLDFEHFLPMLQTvaKNKDQGTYEDYVEGLRVFDKEGNGTVMGAEIRHVLVT 80
Cdd:PTZ00184  37 MRSLGQNPTEAELQDMINEVDAD--GNGTIDFPEFLTLMAR--KMKDTDSEEEIKEAFKVFDRDGNGFISAAELRHVMTN 112

                         90       100       110
                 ....*....|....*....|....*....|....*.
gi 954170003  81 LGEKMTEEEV-EMLVAGHEDSNGCINYEAFVRHILS 115
Cdd:PTZ00184 113 LGEKLTDEEVdEMIREADVDGDGQINYEEFVKMMMS 148
 
Name Accession Description Interval E-value
PTZ00184 PTZ00184
calmodulin; Provisional
 1-115 6.84e-27

calmodulin; Provisional


Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 96.75  E-value: 6.84e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 954170003   1 MRALGQNPTNAEVLKVLGNPKSDemNVKVLDFEHFLPMLQTvaKNKDQGTYEDYVEGLRVFDKEGNGTVMGAEIRHVLVT 80
Cdd:PTZ00184  37 MRSLGQNPTEAELQDMINEVDAD--GNGTIDFPEFLTLMAR--KMKDTDSEEEIKEAFKVFDRDGNGFISAAELRHVMTN 112

                         90       100       110
                 ....*....|....*....|....*....|....*.
gi 954170003  81 LGEKMTEEEV-EMLVAGHEDSNGCINYEAFVRHILS 115
Cdd:PTZ00184 113 LGEKLTDEEVdEMIREADVDGDGQINYEEFVKMMMS 148
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 59-113 1.41e-08

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 47.54  E-value: 1.41e-08
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 954170003  59 RVFDKEGNGTVMGAEIRHVLVTLGEKMTEEEV-EMLVAGHEDSNGCINYEAFVRHI 113
Cdd:cd00051    7 RLFDKDGDGTISADELKAALKSLGEGLSEEEIdEMIREVDKDGDGKIDFEEFLELM 62
 
Name Accession Description Interval E-value
PTZ00184 PTZ00184
calmodulin; Provisional
 1-115 6.84e-27

calmodulin; Provisional


Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 96.75  E-value: 6.84e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 954170003   1 MRALGQNPTNAEVLKVLGNPKSDemNVKVLDFEHFLPMLQTvaKNKDQGTYEDYVEGLRVFDKEGNGTVMGAEIRHVLVT 80
Cdd:PTZ00184  37 MRSLGQNPTEAELQDMINEVDAD--GNGTIDFPEFLTLMAR--KMKDTDSEEEIKEAFKVFDRDGNGFISAAELRHVMTN 112

                         90       100       110
                 ....*....|....*....|....*....|....*.
gi 954170003  81 LGEKMTEEEV-EMLVAGHEDSNGCINYEAFVRHILS 115
Cdd:PTZ00184 113 LGEKLTDEEVdEMIREADVDGDGQINYEEFVKMMMS 148
PTZ00183 PTZ00183
centrin; Provisional
 1-111 9.39e-10

centrin; Provisional


Pssm-ID: 185503 [Multi-domain]  Cd Length: 158  Bit Score: 52.77  E-value: 9.39e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 954170003   1 MRALGQNPTNAEVLKVLGNPKSDemNVKVLDFEHFLPMLQTVAKNKDqgTYEDYVEGLRVFDKEGNGTVMGAEIRHVLVT 80
Cdd:PTZ00183  43 MRSLGFEPKKEEIKQMIADVDKD--GSGKIDFEEFLDIMTKKLGERD--PREEILKAFRLFDDDKTGKISLKNLKRVAKE 118

                         90       100       110
                 ....*....|....*....|....*....|..
gi 954170003  81 LGEKMTEEEV-EMLVAGHEDSNGCINYEAFVR 111
Cdd:PTZ00183 119 LGETITDEELqEMIDEADRNGDGEISEEEFYR 150
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 59-113 1.41e-08

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 47.54  E-value: 1.41e-08
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 954170003  59 RVFDKEGNGTVMGAEIRHVLVTLGEKMTEEEV-EMLVAGHEDSNGCINYEAFVRHI 113
Cdd:cd00051    7 RLFDKDGDGTISADELKAALKSLGEGLSEEEIdEMIREVDKDGDGKIDFEEFLELM 62
PTZ00184 PTZ00184
calmodulin; Provisional
 52-111 3.25e-04

calmodulin; Provisional


Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 37.82  E-value: 3.25e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 954170003  52 EDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGEKMTEEEV-EMLVAGHEDSNGCINYEAFVR 111
Cdd:PTZ00184  11 AEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELqDMINEVDADGNGTIDFPEFLT 71
EFh_parvalbumin_like cd16251
EF-hand, calcium binding motif, found in parvalbumin-like EF-hand family; The family includes ...
 46-109 1.26e-03

EF-hand, calcium binding motif, found in parvalbumin-like EF-hand family; The family includes alpha- and beta-parvalbumins, and a group of uncharacterized calglandulin-like proteins. Parvalbumins are small, acidic, cytosolic EF-hand-containing Ca2+-buffer and Ca2+ transporter/shuttle proteins belonging to EF-hand superfamily. They are expressed by vertebrates in fast-twitch muscle cells, specific neurons of the central and peripheral nervous system, sensory cells of the mammalian auditory organ (Corti's cell), and some other cells, and characterized by the presence of three consecutive EF-hand motifs (helix-loop-helix) called AB, CD, and EF, but only CD and EF can chelate metal ions, such as Ca2+ and Mg2+. Thus, they may play an additional role in Mg2+ handling. Moreover, parvalbumins represent one of the major animal allergens. In metal-bound states, parvalbumins possess a rigid and stable tertiary structure and display strong allergenicity. In contrast, the metal-free parvalbumins are intrinsically disordered, and the loss of metal ions results in a conformational change that decreases their IgE binding capacity. Furthermore, parvalbumins have been widely used as a neuronal marker for a variety of functional brain systems. They also function as a Ca2+ shuttle transporting Ca2+ from troponin-C (TnC) to the sarcoplasmic reticulum (SR) Ca2+ pump during muscle relaxation. Thus they may facilitate myocardial relaxation and play important roles in cardiac diastolic dysfunction. Parvalbumins consists of alpha- and beta- sublineages, which can be distinguished on the basis of isoelectric point (pI > 5 for alpha; pI


Pssm-ID: 319994 [Multi-domain]  Cd Length: 101  Bit Score: 35.59  E-value: 1.26e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 954170003  46 KDQGTYEDYVEGLRVFDKEGNGTVMGAEIRHVLVTL---GEKMTEEEVEMLV-AGHEDSNGCINYEAF 109
Cdd:cd16251   28 LKQKSEDQIKKVFQILDKDKSGFIEEEELKYILKGFsiaGRDLTDEETKALLaAGDTDGDGKIGVEEF 95
EFh_calglandulin_like cd16252
EF-hand, calcium binding motif, found in uncharacterized calglandulin-like proteins; The ...
 29-109 3.60e-03

EF-hand, calcium binding motif, found in uncharacterized calglandulin-like proteins; The family corresponds to a group of uncharacterized calglandulin-like proteins. Although their biological function remain unclear, they show high sequence similarity with human calglandulin-like protein GAGLP, which is an ortholog of calglandulin from the venom glands of Bothrops insularis snake. Both GAGLP and calglandulin are putative Ca2+-binding proteins with four EF-hand motifs. However, members in this family contain only three EF-hand motifs. In this point, they may belong to the parvalbumin-like EF-hand family, which is characterized by the presence of three consecutive EF-hand motifs (helix-loop-helix).


Pssm-ID: 319995 [Multi-domain]  Cd Length: 106  Bit Score: 34.43  E-value: 3.60e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 954170003  29 VLDFEHFLPMLQTVAKNKDQGtyEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGEKM-----TEEEVE-MLVAGHEDSNG 102
Cdd:cd16252   16 SFNYSKFFEYMQKFQTSEQQE--EAIRKAFQMLDKDKSGFIEWNEIKYILSTVPSSMpvaplSDEEAEaMIQAADTDGDG 93


                 ....*..
gi 954170003 103 CINYEAF 109
Cdd:cd16252   94 RIDFQEF 100
EFh_PEF_CAPN1_like cd16189
Penta-EF hand, calcium binding motifs, found in mu-type calpain (CAPN1), m-type calpain (CAPN2) ...
 52-111 7.59e-03

Penta-EF hand, calcium binding motifs, found in mu-type calpain (CAPN1), m-type calpain (CAPN2), and similar proteins; The family includes mu-type calpain (CAPN1) and m-type calpain (CAPN2), both of which are ubiquitously expressed 80-kDa Ca2+-dependent intracellular cysteine proteases that contain a short N-terminal anchor helix, followed by a calpain cysteine protease (CysPc) domain, a C2-domain-like (C2L) domain, and a C-terminal Ca2+-binding penta-EF-hand (PEF) domain. The catalytic subunit CAPN1 or CAPN2 in complex with a regulatory subunit encoded by CAPNS1 forms a mu- or m-calpain heterodimer, respectively.


Pssm-ID: 320064 [Multi-domain]  Cd Length: 168  Bit Score: 34.25  E-value: 7.59e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 954170003  52 EDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGEKMTEEEVEMLVAGHEDSNGCINYEAFVR 111
Cdd:cd16189   73 QKYLKIYKKFDTDGSGTMSSYEMRLALEEAGFKLNNQLHQVLVARYADQELTIDFDNFVR 132
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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