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Conserved domains on  [gi|566006168|ref|NP_001274360|]
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arfaptin-1 isoform 1 [Homo sapiens]

Protein Classification

arfaptin family protein( domain architecture ID 10166602)

arfaptin family protein may mediate cross-talk between Rac, a member of the Rho family GTPases, and Arf (ADP-ribosylation factor) small GTPases

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
BAR_Arfaptin cd07660
The Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin; The BAR domain of Arfaptin-like proteins, ...
155-355 2.46e-129

The Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin; The BAR domain of Arfaptin-like proteins, also called the Arfaptin domain, is a dimerization and lipid binding module that can detect and drive membrane curvature. Arfaptins are ubiquitously expressed proteins implicated in mediating cross-talk between Rac, a member of the Rho family GTPases, and Arf (ADP-ribosylation factor) small GTPases. Arfaptins bind to GTP-bound Arf1, Arf5, and Arf6, with strongest binding to GTP-Arf1. Arfaptins also bind to Rac-GTP and Rac-GDP with similar affinities. The Arfs are thought to bind to the same surface as Rac, and their binding is mutually exclusive. Mammals contain at least two isoforms of Arfaptin. Arfaptin 1 has been shown to inhibit the activation of Arf-dependent phospholipase D (PLD) and the secretion of matrix metalloproteinase-9 (MMP-9), an enzyme implicated in cancer invasiveness and metastasis. Arfaptin 2 regulates the aggregation of the protein huntingtin, which is implicated in Huntington disease. Arfaptins are single-domain proteins with a BAR-like structure. BAR domains form dimers that bind to membranes, induce membrane bending and curvature, and may also be involved in protein-protein interactions.


:

Pssm-ID: 153344  Cd Length: 201  Bit Score: 368.96  E-value: 2.46e-129
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168 155 DLELEAQIDILRDNKKKYENILKLAQTLSTQLFQMVHTQRQLGDAFADLSLKSLELHEEFGYNADTQKLLAKNGETLLGA 234
Cdd:cd07660    1 DLELEAQIEVLRDTQRKYESVLRLARALASQFYQMLQTQKALGDAFADLSQKSPELQEEFTYNAETQKLLCKNGETLLGA 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168 235 INFFIASVNTLVNKTIEDTLMTVKQYESARIEYDAYRTDLEELNLGPRDANTLPKIEQSQHLFQAHKEKYDKMRNDVSVK 314
Cdd:cd07660   81 LNFFVSSLNTLVNKTMEDTLMTVKQYESARIEYDAYRNDLEALNLGPRDAATSARLEEAQRRFQAHKDKYEKLRNDVSVK 160
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|.
gi 566006168 315 LKFLEENKVKVLHNQLVLFHNAIAAYFAGNQKQLEQTLKQF 355
Cdd:cd07660  161 LKFLEENKVKVMHKQLLLFHNAISAYFSGNQKQLEQTLKQF 201
 
Name Accession Description Interval E-value
BAR_Arfaptin cd07660
The Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin; The BAR domain of Arfaptin-like proteins, ...
155-355 2.46e-129

The Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin; The BAR domain of Arfaptin-like proteins, also called the Arfaptin domain, is a dimerization and lipid binding module that can detect and drive membrane curvature. Arfaptins are ubiquitously expressed proteins implicated in mediating cross-talk between Rac, a member of the Rho family GTPases, and Arf (ADP-ribosylation factor) small GTPases. Arfaptins bind to GTP-bound Arf1, Arf5, and Arf6, with strongest binding to GTP-Arf1. Arfaptins also bind to Rac-GTP and Rac-GDP with similar affinities. The Arfs are thought to bind to the same surface as Rac, and their binding is mutually exclusive. Mammals contain at least two isoforms of Arfaptin. Arfaptin 1 has been shown to inhibit the activation of Arf-dependent phospholipase D (PLD) and the secretion of matrix metalloproteinase-9 (MMP-9), an enzyme implicated in cancer invasiveness and metastasis. Arfaptin 2 regulates the aggregation of the protein huntingtin, which is implicated in Huntington disease. Arfaptins are single-domain proteins with a BAR-like structure. BAR domains form dimers that bind to membranes, induce membrane bending and curvature, and may also be involved in protein-protein interactions.


Pssm-ID: 153344  Cd Length: 201  Bit Score: 368.96  E-value: 2.46e-129
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168 155 DLELEAQIDILRDNKKKYENILKLAQTLSTQLFQMVHTQRQLGDAFADLSLKSLELHEEFGYNADTQKLLAKNGETLLGA 234
Cdd:cd07660    1 DLELEAQIEVLRDTQRKYESVLRLARALASQFYQMLQTQKALGDAFADLSQKSPELQEEFTYNAETQKLLCKNGETLLGA 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168 235 INFFIASVNTLVNKTIEDTLMTVKQYESARIEYDAYRTDLEELNLGPRDANTLPKIEQSQHLFQAHKEKYDKMRNDVSVK 314
Cdd:cd07660   81 LNFFVSSLNTLVNKTMEDTLMTVKQYESARIEYDAYRNDLEALNLGPRDAATSARLEEAQRRFQAHKDKYEKLRNDVSVK 160
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|.
gi 566006168 315 LKFLEENKVKVLHNQLVLFHNAIAAYFAGNQKQLEQTLKQF 355
Cdd:cd07660  161 LKFLEENKVKVMHKQLLLFHNAISAYFSGNQKQLEQTLKQF 201
Arfaptin smart01015
Arfaptin-like domain; Arfaptin interacts with ARF1, a small GTPase involved in vesicle budding ...
134-348 1.72e-106

Arfaptin-like domain; Arfaptin interacts with ARF1, a small GTPase involved in vesicle budding at the Golgi complex and immature secretory granules. The structure of arfaptin shows that upon binding to a small GTPase, arfaptin forms an elongated, crescent-shaped dimer of three-helix coiled-coils. The N-terminal region of ICA69 is similar to arfaptin.


Pssm-ID: 214974  Cd Length: 217  Bit Score: 311.51  E-value: 1.72e-106
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168   134 NTYKCTRQIISEKLGRGSR----TVDLELEAQIDILRDNKKKYENILKLAQTLSTQLFQMVHTQRQLGDAFADLSLKSLE 209
Cdd:smart01015   1 KTYKKTKQVLIEKLGKKEDehvvASDAELDAKLELLRSTQRTYEDLLKLIEKYQQRLCNLSQTENELGDFFRDLSEKDPT 80
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168   210 LhEEFGYNADTQKLLAKNGETLLGAINFFIASVNTLVNKTIEDTLMTVKQYESARIEYDAYRTDLEElNLGPRDANTLPK 289
Cdd:smart01015  81 L-KAFGMMAETQKALCKSGEQLLAPLNPFISDVNTFVNKAIEDTLLTIKRYEDARTEYRAWMKDVSE-ELDPEEYKQLEK 158
                          170       180       190       200       210
                   ....*....|....*....|....*....|....*....|....*....|....*....
gi 566006168   290 IEQSQHLFQAHKEKYDKMRNDVSVKLKFLEENKVKVLHNQLVLFHNAIAAYFAGNQKQL 348
Cdd:smart01015 159 FRKAQRQVQEAKAKFEKLRNDVCQKVDLLEASRVNVLSHQLLLFQNALAAYWEKTAHAL 217
Arfaptin pfam06456
Arfaptin-like domain; Arfaptin interacts with ARF1, a small GTPase involved in vesicle budding ...
153-348 5.35e-91

Arfaptin-like domain; Arfaptin interacts with ARF1, a small GTPase involved in vesicle budding at the Golgi complex and immature secretory granules. The structure of arfaptin shows that upon binding to a small GTPase, arfaptin forms an elongated, crescent-shaped dimer of three-helix coiled-coils. The N-terminal region of ICA69 is similar to arfaptin.


Pssm-ID: 399453  Cd Length: 207  Bit Score: 271.92  E-value: 5.35e-91
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168  153 TVDLELEAQIDILRDNKKKYENILKLAQTLSTQLFQMVHTQRQLGDAFADLSL--KSLELHEEFGYNADTQKLLAKNGET 230
Cdd:pfam06456   8 TSDDELDAKLEVLRSIQRTYLGLVKLARNYSKRLYDLSQTQKELGDFFKDLGKheKQQAAGEAFTAFGETHRFLAKQGLA 87
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168  231 LLGAINFFIASVNTLVNKTIEDTLMTVKQYESARIEYDAYRTDLEELN--LGPRDANTLPKIEQSQHLFQAHKEKYDKMR 308
Cdd:pfam06456  88 LLVPLNRFISSVNTFVNKAIPDTLLTIKRYEDARTEYRAYLLWMKEASdeLDPDVAKQMPKFRVAQGNYQEAKAKFDKLR 167
                         170       180       190       200
                  ....*....|....*....|....*....|....*....|
gi 566006168  309 NDVSVKLKFLEENKVKVLHNQLVLFHNAIAAYFAGNQKQL 348
Cdd:pfam06456 168 TDVLQKMDLLEANRINVLSHQLTLYQNTLAAYYSKNAKAL 207
 
Name Accession Description Interval E-value
BAR_Arfaptin cd07660
The Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin; The BAR domain of Arfaptin-like proteins, ...
155-355 2.46e-129

The Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin; The BAR domain of Arfaptin-like proteins, also called the Arfaptin domain, is a dimerization and lipid binding module that can detect and drive membrane curvature. Arfaptins are ubiquitously expressed proteins implicated in mediating cross-talk between Rac, a member of the Rho family GTPases, and Arf (ADP-ribosylation factor) small GTPases. Arfaptins bind to GTP-bound Arf1, Arf5, and Arf6, with strongest binding to GTP-Arf1. Arfaptins also bind to Rac-GTP and Rac-GDP with similar affinities. The Arfs are thought to bind to the same surface as Rac, and their binding is mutually exclusive. Mammals contain at least two isoforms of Arfaptin. Arfaptin 1 has been shown to inhibit the activation of Arf-dependent phospholipase D (PLD) and the secretion of matrix metalloproteinase-9 (MMP-9), an enzyme implicated in cancer invasiveness and metastasis. Arfaptin 2 regulates the aggregation of the protein huntingtin, which is implicated in Huntington disease. Arfaptins are single-domain proteins with a BAR-like structure. BAR domains form dimers that bind to membranes, induce membrane bending and curvature, and may also be involved in protein-protein interactions.


Pssm-ID: 153344  Cd Length: 201  Bit Score: 368.96  E-value: 2.46e-129
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168 155 DLELEAQIDILRDNKKKYENILKLAQTLSTQLFQMVHTQRQLGDAFADLSLKSLELHEEFGYNADTQKLLAKNGETLLGA 234
Cdd:cd07660    1 DLELEAQIEVLRDTQRKYESVLRLARALASQFYQMLQTQKALGDAFADLSQKSPELQEEFTYNAETQKLLCKNGETLLGA 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168 235 INFFIASVNTLVNKTIEDTLMTVKQYESARIEYDAYRTDLEELNLGPRDANTLPKIEQSQHLFQAHKEKYDKMRNDVSVK 314
Cdd:cd07660   81 LNFFVSSLNTLVNKTMEDTLMTVKQYESARIEYDAYRNDLEALNLGPRDAATSARLEEAQRRFQAHKDKYEKLRNDVSVK 160
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|.
gi 566006168 315 LKFLEENKVKVLHNQLVLFHNAIAAYFAGNQKQLEQTLKQF 355
Cdd:cd07660  161 LKFLEENKVKVMHKQLLLFHNAISAYFSGNQKQLEQTLKQF 201
Arfaptin smart01015
Arfaptin-like domain; Arfaptin interacts with ARF1, a small GTPase involved in vesicle budding ...
134-348 1.72e-106

Arfaptin-like domain; Arfaptin interacts with ARF1, a small GTPase involved in vesicle budding at the Golgi complex and immature secretory granules. The structure of arfaptin shows that upon binding to a small GTPase, arfaptin forms an elongated, crescent-shaped dimer of three-helix coiled-coils. The N-terminal region of ICA69 is similar to arfaptin.


Pssm-ID: 214974  Cd Length: 217  Bit Score: 311.51  E-value: 1.72e-106
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168   134 NTYKCTRQIISEKLGRGSR----TVDLELEAQIDILRDNKKKYENILKLAQTLSTQLFQMVHTQRQLGDAFADLSLKSLE 209
Cdd:smart01015   1 KTYKKTKQVLIEKLGKKEDehvvASDAELDAKLELLRSTQRTYEDLLKLIEKYQQRLCNLSQTENELGDFFRDLSEKDPT 80
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168   210 LhEEFGYNADTQKLLAKNGETLLGAINFFIASVNTLVNKTIEDTLMTVKQYESARIEYDAYRTDLEElNLGPRDANTLPK 289
Cdd:smart01015  81 L-KAFGMMAETQKALCKSGEQLLAPLNPFISDVNTFVNKAIEDTLLTIKRYEDARTEYRAWMKDVSE-ELDPEEYKQLEK 158
                          170       180       190       200       210
                   ....*....|....*....|....*....|....*....|....*....|....*....
gi 566006168   290 IEQSQHLFQAHKEKYDKMRNDVSVKLKFLEENKVKVLHNQLVLFHNAIAAYFAGNQKQL 348
Cdd:smart01015 159 FRKAQRQVQEAKAKFEKLRNDVCQKVDLLEASRVNVLSHQLLLFQNALAAYWEKTAHAL 217
Arfaptin pfam06456
Arfaptin-like domain; Arfaptin interacts with ARF1, a small GTPase involved in vesicle budding ...
153-348 5.35e-91

Arfaptin-like domain; Arfaptin interacts with ARF1, a small GTPase involved in vesicle budding at the Golgi complex and immature secretory granules. The structure of arfaptin shows that upon binding to a small GTPase, arfaptin forms an elongated, crescent-shaped dimer of three-helix coiled-coils. The N-terminal region of ICA69 is similar to arfaptin.


Pssm-ID: 399453  Cd Length: 207  Bit Score: 271.92  E-value: 5.35e-91
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168  153 TVDLELEAQIDILRDNKKKYENILKLAQTLSTQLFQMVHTQRQLGDAFADLSL--KSLELHEEFGYNADTQKLLAKNGET 230
Cdd:pfam06456   8 TSDDELDAKLEVLRSIQRTYLGLVKLARNYSKRLYDLSQTQKELGDFFKDLGKheKQQAAGEAFTAFGETHRFLAKQGLA 87
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168  231 LLGAINFFIASVNTLVNKTIEDTLMTVKQYESARIEYDAYRTDLEELN--LGPRDANTLPKIEQSQHLFQAHKEKYDKMR 308
Cdd:pfam06456  88 LLVPLNRFISSVNTFVNKAIPDTLLTIKRYEDARTEYRAYLLWMKEASdeLDPDVAKQMPKFRVAQGNYQEAKAKFDKLR 167
                         170       180       190       200
                  ....*....|....*....|....*....|....*....|
gi 566006168  309 NDVSVKLKFLEENKVKVLHNQLVLFHNAIAAYFAGNQKQL 348
Cdd:pfam06456 168 TDVLQKMDLLEANRINVLSHQLTLYQNTLAAYYSKNAKAL 207
BAR_Arfaptin_like cd00011
The Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin-like proteins, a dimerization module that ...
155-354 8.02e-70

The Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin-like proteins, a dimerization module that binds and bends membranes; The BAR domain of Arfaptin-like proteins, also called the Arfaptin domain, is a dimerization, lipid binding and curvature sensing module present in Arfaptins, PICK1, ICA69, and similar proteins. Arfaptins are ubiquitously expressed proteins implicated in mediating cross-talk between Rac, a member of the Rho family GTPases, and Arf (ADP-ribosylation factor) small GTPases. Arfaptins bind to GTP-bound Arf1, Arf5, and Arf6, with strongest binding to GTP-Arf1. Arfaptins also binds to Rac-GTP and Rac-GDP with similar affinities. The Arfs are thought to bind to the same surface as Rac, and their binding is mutually exclusive. Protein Interacting with C Kinase 1 (PICK1) plays a key role in the trafficking of AMPA receptors, which are critical for regulating synaptic strength and may be important in cellular processes involved in learning and memory. Islet cell autoantigen 69-kDa (ICA69) is a diabetes-associated autoantigen that is involved in membrane trafficking at the Golgi complex in neurosecretory cells. ICA69 associates with PICK1 through their BAR domains to form a heterodimer which is involved in regulating the synaptic targeting and surface expression of AMPA receptors. BAR domains form dimers that bind to membranes, induce membrane bending and curvature, and may also be involved in protein-protein interactions.


Pssm-ID: 153270  Cd Length: 203  Bit Score: 217.87  E-value: 8.02e-70
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168 155 DLELEAQIDILRDNKKKYENILKLAQTLSTQLFQMVHTQRQLGDAFADLSLKSLEL-HEEFGYNADTQKLLAKNGETLLG 233
Cdd:cd00011    1 DLELELQLELLRETKRKYESVLQLGRALTAHLYSLSQTQHALGDAFADLSQKDPELaGEEFGYNAEAQKLLCKNGETLLG 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168 234 AINFFIASVNTLVNKTIEDTLMTVKQYESARIEYDAYRTDLEELNLGPRD--ANTLPKIEQSQHLFQAHKEKYDKMRNDV 311
Cdd:cd00011   81 AVNFFVSSINTLVTKAIEDTLLTVKQYEAARLEYDAYRLDLKELSLEPRDdtAGTRGRLRSAQATFQEHRDKFEKLRGDV 160
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|...
gi 566006168 312 SVKLKFLEENKVKVLHNQLVLFHNAIAAYFAGNQKQLEQTLKQ 354
Cdd:cd00011  161 AIKLKFLEENKIKVMHKQLLLFHNTVSAYFAGNQKVLEQTLQQ 203
BAR cd07307
The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects ...
165-354 1.39e-20

The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature; BAR domains are dimerization, lipid binding and curvature sensing modules found in many different proteins with diverse functions including organelle biogenesis, membrane trafficking or remodeling, and cell division and migration. Mutations in BAR containing proteins have been linked to diseases and their inactivation in cells leads to altered membrane dynamics. A BAR domain with an additional N-terminal amphipathic helix (an N-BAR) can drive membrane curvature. These N-BAR domains are found in amphiphysins and endophilins, among others. BAR domains are also frequently found alongside domains that determine lipid specificity, such as the Pleckstrin Homology (PH) and Phox Homology (PX) domains which are present in beta centaurins (ACAPs and ASAPs) and sorting nexins, respectively. A FES-CIP4 Homology (FCH) domain together with a coiled coil region is called the F-BAR domain and is present in Pombe/Cdc15 homology (PCH) family proteins, which include Fes/Fes tyrosine kinases, PACSIN or syndapin, CIP4-like proteins, and srGAPs, among others. The Inverse (I)-BAR or IRSp53/MIM homology Domain (IMD) is found in multi-domain proteins, such as IRSp53 and MIM, that act as scaffolding proteins and transducers of a variety of signaling pathways that link membrane dynamics and the underlying actin cytoskeleton. BAR domains form dimers that bind to membranes, induce membrane bending and curvature, and may also be involved in protein-protein interactions. The I-BAR domain induces membrane protrusions in the opposite direction compared to classical BAR and F-BAR domains, which produce membrane invaginations. BAR domains that also serve as protein interaction domains include those of arfaptin and OPHN1-like proteins, among others, which bind to Rac and Rho GAP domains, respectively.


Pssm-ID: 153271 [Multi-domain]  Cd Length: 194  Bit Score: 88.27  E-value: 1.39e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168 165 LRDNKKKYENILKLAQTLSTQLFQMVHTQRQLGDAFADLSLKSLELH-----EEFGYNADTQKLLAKNGETLLGAI-NFF 238
Cdd:cd07307    2 LDELEKLLKKLIKDTKKLLDSLKELPAAAEKLSEALQELGKELPDLSntdlgEALEKFGKIQKELEEFRDQLEQKLeNKV 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168 239 IASVNTLVNKTIEDTLMTVKQYESARIEYDAYRTDLEELNLGPRDANTLPKIEQsqhLFQAHKEKYDKMRNDVSVKLKFL 318
Cdd:cd07307   82 IEPLKEYLKKDLKEIKKRRKKLDKARLDYDAAREKLKKLRKKKKDSSKLAEAEE---ELQEAKEKYEELREELIEDLNKL 158
                        170       180       190
                 ....*....|....*....|....*....|....*.
gi 566006168 319 EENKVKVLHNQLVLFHNAIAAYFAGNQKQLEQTLKQ 354
Cdd:cd07307  159 EEKRKELFLSLLLSFIEAQSEFFKEVLKILEQLLPY 194
BAR_PICK1 cd07659
The Bin/Amphiphysin/Rvs (BAR) domain of Protein Interacting with C Kinase 1; The BAR domain of ...
158-340 1.88e-17

The Bin/Amphiphysin/Rvs (BAR) domain of Protein Interacting with C Kinase 1; The BAR domain of Arfaptin-like proteins, also called the Arfaptin domain, is a dimerization and lipid binding module that can detect and drive membrane curvature. Protein Interacting with C Kinase 1 (PICK1), also called Protein kinase C-alpha-binding protein, is highly expressed in brain and testes. PICK1 plays a key role in the trafficking of AMPA receptors, which are critical for regulating synaptic strength and may be important in cellular processes involved in learning and memory. PICK1 is also critical in the early stages of spermiogenesis. Mice deficient in PICK1 are infertile and show characteristics of the human disease globozoospermia such as round-headed sperm, reduced sperm count, and severely impaired sperm motility. PICK1 may also be involved in the neuropathogenesis of schizophrenia. PICK1 contains an N-terminal PDZ domain and a C-terminal BAR domain. BAR domains form dimers that bind to membranes, induce membrane bending and curvature, and may also be involved in protein-protein interactions. The BAR domain of PICK1 is necessary for its membrane localization and activation.


Pssm-ID: 153343  Cd Length: 215  Bit Score: 80.06  E-value: 1.88e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168 158 LEAQIDILRDNKKKYENILKLAQTLSTQLFQMVHTQRQLGDAFADLSLKSLE--LHEEFGYNADTQKLLAKNGETLLGAI 235
Cdd:cd07659    4 LVKKLEELEQTAELYKGLVEHTKRLLRAFYALSQTHKEFGDLFANIGVREPQpaASEAFTKFGEAHRSIEKFGIELLKTL 83
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168 236 NFFIASVNTLVNKTIEDTLMTVKQYESARIEYDAYRTDLEELnlgprDANTLPKIEQSQHLF----------------QA 299
Cdd:cd07659   84 KPMLSDLGTYLNKAIPDTKLTIKKYADVKFEYLSYCLKVKEM-----DDEEYSYAALDEPLYrvetgnyeyrlilrcrQE 158
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|.
gi 566006168 300 HKEKYDKMRNDVSVKLKFLEENKVKVLHNQLVLFHNAIAAY 340
Cdd:cd07659  159 ARARFAKLRQDVLEKLELLDQKHVQDIVFQLQRFVSALSEY 199
BAR_ICA69 cd07661
The Bin/Amphiphysin/Rvs (BAR) domain of Islet Cell Autoantigen 69-kDa; The BAR domain of ...
242-355 4.57e-06

The Bin/Amphiphysin/Rvs (BAR) domain of Islet Cell Autoantigen 69-kDa; The BAR domain of Arfaptin-like proteins, also called the Arfaptin domain, is a dimerization and lipid binding module that can detect and drive membrane curvature. Islet cell autoantigen 69-kDa (ICA69) is a diabetes-associated autoantigen that is highly expressed in brain and beta cells. It is involved in membrane trafficking at the Golgi complex in neurosecretory cells. It is coexpressed with Protein Interacting with C Kinase 1 (PICK1), also a the BAR domain containing protein, in many tissues at different developmental stages. In neurons, ICA69 colocalizes with PICK1 in cell bodies and dendrites but is absent in synapses where PICK1 is enriched. ICA69 contains an N-terminal BAR domain and a conserved C-terminal domain of unknown function. BAR domains form dimers that bind to membranes, induce membrane bending and curvature, and may also be involved in protein-protein interactions. ICA69 associates with PICK1 through their BAR domains to form a heterodimer which is involved in regulating the synaptic targeting and surface expression of AMPA receptors. Autoantibodies against ICA69 have been identified in patients with insulin-dependent diabetes mellitus, rheumatoid arthritis, and primary Sjogren's syndrome. ICA69 has also been shown to be released by pancreatic cancer cells.


Pssm-ID: 153345  Cd Length: 204  Bit Score: 47.09  E-value: 4.57e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168 242 VNTLVNKTIEDTLMTVKQYESARIEYDA----YRTDLEELNlgPRDANTLPKIEQSQHLFQAHKEKYDKMRNDVSVKLKF 317
Cdd:cd07661   89 VETFRERAIADTLQTIQRMEKCRTEYRAallwMKSVSQELD--PDTYKQLEKFRKAQAQVRSAKERFDKLKMDVCQKVDL 166
                         90       100       110
                 ....*....|....*....|....*....|....*...
gi 566006168 318 LEENKVKVLHNQLVLFHNAIAAYFAGNQKQLEQTLKQF 355
Cdd:cd07661  167 LGASRCNLLSHALVTYQNTLLQFWEKTSRTMATIHEAF 204
BAR_SNX cd07596
The Bin/Amphiphysin/Rvs (BAR) domain of Sorting Nexins; BAR domains are dimerization, lipid ...
142-355 1.82e-03

The Bin/Amphiphysin/Rvs (BAR) domain of Sorting Nexins; BAR domains are dimerization, lipid binding and curvature sensing modules found in many different proteins with diverse functions. Sorting nexins (SNXs) are Phox homology (PX) domain containing proteins that are involved in regulating membrane traffic and protein sorting in the endosomal system. SNXs differ from each other in their lipid-binding specificity, subcellular localization and specific function in the endocytic pathway. A subset of SNXs also contain BAR domains. The PX-BAR structural unit determines the specific membrane targeting of SNXs. BAR domains form dimers that bind to membranes, induce membrane bending and curvature, and may also be involved in protein-protein interactions.


Pssm-ID: 153280 [Multi-domain]  Cd Length: 218  Bit Score: 39.26  E-value: 1.82e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168 142 IISEKLGRGSRTVDLELEAQIDILRDNKKKYENILKLAQTLSTQLFQMVHTQRQLGDAFADLSlkslelheeFGYNADTQ 221
Cdd:cd07596   15 KLEEQLKKLSKQAQRLVKRRRELGSALGEFGKALIKLAKCEEEVGGELGEALSKLGKAAEELS---------SLSEAQAN 85
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 566006168 222 KLLAKNGETLLGAINFFIAsvntlVNKTIEDTLMTVKQYESARIEYDAYRTDLEELNLGP-----RDANTLPKIEQSQHL 296
Cdd:cd07596   86 QELVKLLEPLKEYLRYCQA-----VKETLDDRADALLTLQSLKKDLASKKAQLEKLKAAPgikpaKVEELEEELEEAESA 160
                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 566006168 297 FQAHKEKYDKMRNDVSVKLKFLEENKVKVLHNQLVLFhnaiAAYFAGNQKQLEQTLKQF 355
Cdd:cd07596  161 LEEARKRYEEISERLKEELKRFHEERARDLKAALKEF----ARLQVQYAEKIAEAWESL 215
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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