Enabled/VASP family EVH1 domain; Ena/VASP family includes proteins such as: Vasodilator-stimulated phosphoprotein (VASP), enabled gene product from Drosophila (Ena), mammalian enabled (Mena) and Ena/VASP-Like protein (EVL) localize to focal adhesions and to sites of actin filament dynamics. These proteins share a common modular organization with a highly conserved N- and C-terminal domains, termed Ena/VASP homology domains 1 and 2 (EVH1 and EVH2), that are separated by a central proline-rich domain. The EVH1 domain binds to other proteins at proline rich sequences. The majority of Ena-VASP type EVH1 domains recognize FPPPP motifs such as in the focal adhesion proteins zyxin and vinculin, and the ActA surface protein of Listeria monocytogenes, however the LIM3 domain of Tes lacks the FPPPP motif but still binds the EVH1 domain of Mena. It has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains. EVH2 mediates oligomerization within the family. The proline-rich region binds SH3 and WW domains as well as profilin, a protein that regulates actin filament dynamics. The EVH1 domains are part of the PH domain superamily. There are 5 EVH1 subfamilies: Enables/VASP, Homer/Vesl, WASP, Dcp1, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 254281247  11 SICQARASVMVYDDTSKKWVPIKpGQQGFSRINIYHNTASSTFRVVGVKLQDQQVVINYSIVKGLKYNQATPTFHQWRDA 90
Cdd:cd01207    1 SVASARASVMVYDDENKRWVPSG-GSQGLSRVQIYHNTRNNTFRVVGRKLQDHEVVINCAILKGLKYNQATPTFHQWRDA 79

                         90       100
                 ....*....|....*....|....*....
gi 254281247  91 RQVYGLNFASKEEATTFSNAMLFALNIMN 119
Cdd:cd01207   80 RQVYGLNFASKEEATEFAQAMLLALERLN 108

Enabled/VASP family EVH1 domain; Ena/VASP family includes proteins such as: Vasodilator-stimulated phosphoprotein (VASP), enabled gene product from Drosophila (Ena), mammalian enabled (Mena) and Ena/VASP-Like protein (EVL) localize to focal adhesions and to sites of actin filament dynamics. These proteins share a common modular organization with a highly conserved N- and C-terminal domains, termed Ena/VASP homology domains 1 and 2 (EVH1 and EVH2), that are separated by a central proline-rich domain. The EVH1 domain binds to other proteins at proline rich sequences. The majority of Ena-VASP type EVH1 domains recognize FPPPP motifs such as in the focal adhesion proteins zyxin and vinculin, and the ActA surface protein of Listeria monocytogenes, however the LIM3 domain of Tes lacks the FPPPP motif but still binds the EVH1 domain of Mena. It has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains. EVH2 mediates oligomerization within the family. The proline-rich region binds SH3 and WW domains as well as profilin, a protein that regulates actin filament dynamics. The EVH1 domains are part of the PH domain superamily. There are 5 EVH1 subfamilies: Enables/VASP, Homer/Vesl, WASP, Dcp1, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 254281247  11 SICQARASVMVYDDTSKKWVPIKpGQQGFSRINIYHNTASSTFRVVGVKLQDQQVVINYSIVKGLKYNQATPTFHQWRDA 90
Cdd:cd01207    1 SVASARASVMVYDDENKRWVPSG-GSQGLSRVQIYHNTRNNTFRVVGRKLQDHEVVINCAILKGLKYNQATPTFHQWRDA 79

                         90       100
                 ....*....|....*....|....*....
gi 254281247  91 RQVYGLNFASKEEATTFSNAMLFALNIMN 119
Cdd:cd01207   80 RQVYGLNFASKEEATEFAQAMLLALERLN 108

WH1 domain; WASp Homology domain 1 (WH1) domain. WASP is the protein that is defective in Wiskott-Aldrich syndrome (WAS). The majority of point mutations occur within the amino- terminal WH1 domain. The metabotropic glutamate receptors mGluR1alpha and mGluR5 bind a protein called homer, which is a WH1 domain homolog. A subset of WH1 domains has been termed a "EVH1" domain and appear to bind a polyproline motif.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 254281247    2 FAFEEFSEQSICQARASVMVYDDTSKK-WVPIKpgqqGFSRINIYHNTASSTFRVVGVKLQDQQVVINYSIVKGLKYNQA 80
Cdd:pfam00568   1 FSALGKKCQTICTAVAQVYLADPDNKRhWIKAK----HSGVVCFVKDSPQNSYFIRLVDIQDGKVIWNQEIYPNMEYNQA 76

                          90       100       110
                  ....*....|....*....|....*....|.
gi 254281247   81 TPTFHQWRDARQVYGLNFASKEEATTFSNAM 111
Cdd:pfam00568  77 RPFFHTFADSRCVYGLNFASEEEATKFAKAV 107

Enabled/VASP family EVH1 domain; Ena/VASP family includes proteins such as: Vasodilator-stimulated phosphoprotein (VASP), enabled gene product from Drosophila (Ena), mammalian enabled (Mena) and Ena/VASP-Like protein (EVL) localize to focal adhesions and to sites of actin filament dynamics. These proteins share a common modular organization with a highly conserved N- and C-terminal domains, termed Ena/VASP homology domains 1 and 2 (EVH1 and EVH2), that are separated by a central proline-rich domain. The EVH1 domain binds to other proteins at proline rich sequences. The majority of Ena-VASP type EVH1 domains recognize FPPPP motifs such as in the focal adhesion proteins zyxin and vinculin, and the ActA surface protein of Listeria monocytogenes, however the LIM3 domain of Tes lacks the FPPPP motif but still binds the EVH1 domain of Mena. It has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains. EVH2 mediates oligomerization within the family. The proline-rich region binds SH3 and WW domains as well as profilin, a protein that regulates actin filament dynamics. The EVH1 domains are part of the PH domain superamily. There are 5 EVH1 subfamilies: Enables/VASP, Homer/Vesl, WASP, Dcp1, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 254281247  11 SICQARASVMVYDDTSKKWVPIKpGQQGFSRINIYHNTASSTFRVVGVKLQDQQVVINYSIVKGLKYNQATPTFHQWRDA 90
Cdd:cd01207    1 SVASARASVMVYDDENKRWVPSG-GSQGLSRVQIYHNTRNNTFRVVGRKLQDHEVVINCAILKGLKYNQATPTFHQWRDA 79

                         90       100
                 ....*....|....*....|....*....
gi 254281247  91 RQVYGLNFASKEEATTFSNAMLFALNIMN 119
Cdd:cd01207   80 RQVYGLNFASKEEATEFAQAMLLALERLN 108

WASP homology region 1; Region of the Wiskott-Aldrich syndrome protein (WASp) that contains point mutations in the majority of patients with WAS. Unknown function. Ena-like WH1 domains bind polyproline-containing peptides, and that Homer contains a WH1 domain.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 254281247     8 SEQSICQARASVMVYDDTSKKWVPIKPGqqGFSRINIYHNTASSTFRVVGVKLQDQqVVINYSIVKGLKYNQATPTFHQW 87
Cdd:smart00461   2 GSQCIILARAVVQLYDADTKKWVPTGEG--GAANLVIDKNQRSYFFRIVGIKGQDK-VIWNQELYKNFKYNQATPTFHQW 78

                           90       100
                   ....*....|....*....|....*...
gi 254281247    88 RDARQVYGLNFASKEEATTFSNAMLFAL 115
Cdd:smart00461  79 ADDKCVYGLNFASEEEAKKFRKKVLKAL 106

Sprouty-related EVH1 domain-containing-like proteins EVH1 domain; The Spred family has the following domains: an N-terminal EVH1 domain, a unique KBD (c-Kit kinase binding) domain which that is phosphorylated by the stem cell factor receptor c-Kit, and a C-terminal cysteine-rich SPR (Sprouty-related) domain which is involved in membrane localization. There are 3 Spred proteins: Spred1 which interacts with both Ras and Raf through its SPR domain; Spred2 which is the most abundant isoform; and Spred3 which has a non-functional KBD and maintains the inhibitory action on Raf. Legius syndrome is caused by heterozygous mutations in Spred1. Both EVH1 and SPR domains are involved in the inhibition of the MAP kinase pathway by Spred proteins. The specific function of the Spred2 EVH1 domain is unknown and there are no known interacting proteins to date. It is thought that its EVH1 domain will have a fourth distinct peptide binding mechanism within the EVH1 family. The EVH1 domains are part of the PH domain superamily. There are 5 EVH1 subfamilies: Enables/VASP, Homer/Vesl, WASP, Dcp1, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 254281247  11 SICQARASVMVYDDTSKKWVPIKPGqqGFSRINIYHNTASST-----FRVVGVKLQDQQVVINYSIVKGLKYNQATPTFH 85
Cdd:cd10574    1 CLVRVRAVVMTRDDSSGGWLPLGGG--GLSIVSVCKVMPEEGaprteYVIHGERIRDKTVVLECTLRKDLVYNKVMPTFH 78

                         90       100       110
                 ....*....|....*....|....*....|.
gi 254281247  86 QWRDARQVYGLNFASKEEATTFSNAMLFALN 116
Cdd:cd10574   79 HWRIGEKKFGLTFQSPADARAFDRGVRRALE 109

Homer/Vesl family proteins EVH1 domain; Homer/Vesl proteins are synaptic scaffolding proteins, required for long-term potentiation, a form of synaptic plasticity thought to underlie memory formation. They contains an N-terminal EVH1 domain and bind to both neurotransmitter receptors, such as the metabotropic group 1 glutamate receptor (mGluR) and to other scaffolding proteins via PPXXF motifs, in order to target them to the synaptic junction. These mGluRs possess a long C-terminal intracellular tail that may be important for subcellular localization of the receptor. The C-terminus is also the site of binding by the immediate early gene (IEG), Homer 1a. In contrast to Homer 1a, other Homer members additionally encode a C-terminal coiled-coil (CC) domain and form multivalent complexes that bind group 1 mGluRs. Homer 1a competes with constitutively expressed CC-Homers to modify the association of group 1 mGluRs with CC-Homer complexes. Since Homer proteins are strikingly enriched at the postsynaptic density (PSD), these observations suggest a role for the Homer family in regulating synaptic metabotropic receptor function. PSD-Zip45 (also named Homer 1c/Vesl-1L) has an EVH1 domain with a longer alpha-helix and its linking part included in the conserved region of Homer 1 (CRH1) interacts with the EVH1 domain of the neighbour CRH1 molecule in the crystal, suggesting that the EVH1 domain recognizes the PPXXF motif found in the binding partners, and the SPLTP sequence (P-motif) in the linking region of the CRH1. The two types of binding are partly overlapped in the EVH1 domain, implying a mechanism to regulate multimerization of Homer 1 family proteins. Homer 2 and Homer 3 are negative regulators of T cell activation. They bind the nuclear factor of activated T cells (NFAT) and compete with calcineurin binding. NFAT plays a critical role in calcium-dependent signaling in other cell types, including muscle and neurons. Homer-NFAT binding is also antagonized by active serine-threonine kinase AKT, enhancing TCR signaling via calcineurin-dependent dephosphorylation of NFAT resulting in changes in cytokine expression and an increase in effector-memory T cell populations in Homer-deficient mice. The EVH1 domains are part of the PH domain superamily. There are 5 EVH1 subfamilies: Enables/VASP, Homer/Vesl, WASP, Dcp1, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 254281247   9 EQSICQARASVMVYDDTSKK-WVPIkpGQQGFsRINIYHNTASSTFRVVGVklQDQQVVINYSIVKGLKYNQATPTFHQW 87
Cdd:cd01206    1 EQPIFTTQAHVFQIDPQTKKsWIPA--SKQAV-TVSFFYDSTRNTYRIISV--EGSKAIINSTITPNMTFTKTSQKFGQW 75

                         90       100
                 ....*....|....*....|....*
gi 254281247  88 RDAR--QVYGLNFASKEEATTFSNA 110
Cdd:cd01206   76 ADSRanTVYGLGFASEAELTKFAEK 100

Ran-binding domain; Domain of apporximately 150 residues that stabilises the GTP-bound form of Ran (the Ras-like nuclear small GTPase).

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 254281247     5 EEfSEQSICQARASVMVYDDTSKKWvpiKPGQQGFSRINiYHNTASSTFRVVGVKLQDQQVVINYSIVKGLKYNQATPTF 84
Cdd:smart00160  16 EE-DEEVIFSARAKLYRFANDKKEW---KERGVGDLKIL-KSKDNGGKVRIVMRRDGVLKVCANHPIFKSMTLKPLAGSN 90

                           90       100       110
                   ....*....|....*....|....*....|....*...
gi 254281247    85 HQWRDARQVYG----------LNFASKEEATTFSNAML 112
Cdd:smart00160  91 RALKWTPEDFAddipklvlyaVRFKTKEEADSFKNIFE 128