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Conserved domains on  [gi|70166599|ref|NP_001002019|]
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pseudouridylate synthase 1 homolog isoform 2 [Homo sapiens]

Protein Classification

pseudouridine synthase family protein( domain architecture ID 10118740)

pseudouridine synthase family protein may catalyze the isomerization of specific uridines in an RNA molecule to pseudouridines; similar to tRNA pseudouridine synthase 1

CATH:  3.30.2350.10
EC:  5.4.99.-
PubMed:  12762017|12756329
SCOP:  3001360

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
PseudoU_synth_PUS1_PUS2 cd02568
Pseudouridine synthase, PUS1/ PUS2 like; This group consists of eukaryotic pseudouridine ...
60-312 3.06e-131

Pseudouridine synthase, PUS1/ PUS2 like; This group consists of eukaryotic pseudouridine synthases similar to Saccharomyces cerevisiae Pus1p, S. cerevisiae Pus2p, Caenorhabditis elegans Pus1p and human PUS1. Pseudouridine synthases catalyze the isomerization of specific uridines in an RNA molecule to pseudouridines (5-ribosyluracil, psi). No cofactors are required. S. cerevisiae Pus1p catalyzes the formation of psi34 and psi36 in the intron-containing tRNAIle, psi35 in the intron-containing tRNATyr, psi27 and/or psi28 in several yeast cytoplasmic tRNAs and, psi44 in U2 small nuclear RNA (U2 snRNA). The presence of the intron is required for the formation of psi 34, 35 and 36. In addition S. cerevisiae PUS1 makes are psi 26, 65 and 67. C. elegans Pus1p does not modify psi44 in U2 snRNA. Mouse Pus1p makes psi27/28 in pre- tRNASer , tRNAVal and tRNAIle, psi 34/36 in tRNAIle and, psi 32 and potentially 67 in tRNAVal. Psi44 in U2 snRNA and psi32 in tRNAs are highly phylogenetically conserved. Psi 26,27,28,34,35,36,65 and 67 in tRNAs are less highly conserved. Mouse Pus1p regulates nuclear receptor activity through pseudouridylation of Steroid Receptor RNA Activator. Missense mutation in human PUS1 causes mitochondrial myopathy and sideroblastic anemia (MLASA).


:

Pssm-ID: 211335 [Multi-domain]  Cd Length: 245  Bit Score: 376.57  E-value: 3.06e-131
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599  60 LLMAYSGKGYHGMQRNVGSsqFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLID--- 136
Cdd:cd02568   1 LLFGYCGTGYHGMQYNPGA--YKTIEGELERALFKAGAISESNAGDPKKIGFSRAARTDKGVHAARNVVSLKVIIDDpeg 78
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599 137 -----DILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAFahkdrdvqdetyrlsaETLQQVNRLLACY 211
Cdd:cd02568  79 lgileDLVEKLNSHLPSDIRVFGITRVTKSFNARKACDSRTYEYLLPTFAL----------------ETLQRFNEILKEY 142
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599 212 KGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVR-EGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSW 290
Cdd:cd02568 143 VGTHNFHNFTVKKKFEDPSANRFIKSFYVSEPFVIeEGLEWISIKIHGQSFMLHQIRKMIGLAIAIVRGGAPESLIELSF 222
                       250       260
                ....*....|....*....|...
gi 70166599 291 GTEKVD-VPKAPGLGLVLERVHF 312
Cdd:cd02568 223 NKDKIIiIPLAPGLGLLLERPHF 245
 
Name Accession Description Interval E-value
PseudoU_synth_PUS1_PUS2 cd02568
Pseudouridine synthase, PUS1/ PUS2 like; This group consists of eukaryotic pseudouridine ...
60-312 3.06e-131

Pseudouridine synthase, PUS1/ PUS2 like; This group consists of eukaryotic pseudouridine synthases similar to Saccharomyces cerevisiae Pus1p, S. cerevisiae Pus2p, Caenorhabditis elegans Pus1p and human PUS1. Pseudouridine synthases catalyze the isomerization of specific uridines in an RNA molecule to pseudouridines (5-ribosyluracil, psi). No cofactors are required. S. cerevisiae Pus1p catalyzes the formation of psi34 and psi36 in the intron-containing tRNAIle, psi35 in the intron-containing tRNATyr, psi27 and/or psi28 in several yeast cytoplasmic tRNAs and, psi44 in U2 small nuclear RNA (U2 snRNA). The presence of the intron is required for the formation of psi 34, 35 and 36. In addition S. cerevisiae PUS1 makes are psi 26, 65 and 67. C. elegans Pus1p does not modify psi44 in U2 snRNA. Mouse Pus1p makes psi27/28 in pre- tRNASer , tRNAVal and tRNAIle, psi 34/36 in tRNAIle and, psi 32 and potentially 67 in tRNAVal. Psi44 in U2 snRNA and psi32 in tRNAs are highly phylogenetically conserved. Psi 26,27,28,34,35,36,65 and 67 in tRNAs are less highly conserved. Mouse Pus1p regulates nuclear receptor activity through pseudouridylation of Steroid Receptor RNA Activator. Missense mutation in human PUS1 causes mitochondrial myopathy and sideroblastic anemia (MLASA).


Pssm-ID: 211335 [Multi-domain]  Cd Length: 245  Bit Score: 376.57  E-value: 3.06e-131
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599  60 LLMAYSGKGYHGMQRNVGSsqFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLID--- 136
Cdd:cd02568   1 LLFGYCGTGYHGMQYNPGA--YKTIEGELERALFKAGAISESNAGDPKKIGFSRAARTDKGVHAARNVVSLKVIIDDpeg 78
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599 137 -----DILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAFahkdrdvqdetyrlsaETLQQVNRLLACY 211
Cdd:cd02568  79 lgileDLVEKLNSHLPSDIRVFGITRVTKSFNARKACDSRTYEYLLPTFAL----------------ETLQRFNEILKEY 142
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599 212 KGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVR-EGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSW 290
Cdd:cd02568 143 VGTHNFHNFTVKKKFEDPSANRFIKSFYVSEPFVIeEGLEWISIKIHGQSFMLHQIRKMIGLAIAIVRGGAPESLIELSF 222
                       250       260
                ....*....|....*....|...
gi 70166599 291 GTEKVD-VPKAPGLGLVLERVHF 312
Cdd:cd02568 223 NKDKIIiIPLAPGLGLLLERPHF 245
hisT_truA TIGR00071
tRNA pseudouridine(38-40) synthase; Members of this family are the tRNA modification enzyme ...
56-307 3.98e-44

tRNA pseudouridine(38-40) synthase; Members of this family are the tRNA modification enzyme TruA, tRNA pseudouridine(38-40) synthase. In a few species (e.g. Bacillus anthracis), TruA is represented by two paralogs. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 272889 [Multi-domain]  Cd Length: 227  Bit Score: 152.85  E-value: 3.98e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599    56 RKIVLLMAYSGKGYHGMQRNVGssqFKTIEDDLVSALVRSGcipenhgedMRKMSFQRCARTDKGVSAAGQVVSLKV--- 132
Cdd:TIGR00071   1 RKIALKIAYDGSNYHGWQRQPN---KRTVQGELEKALEAIG---------KKKITIMSAGRTDKGVHAMGQVISFDTpke 68
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599   133 WLIDDILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPtfafaHKDRDVqdetYRLSAETLQQVnrlLACYK 212
Cdd:TIGR00071  69 IPDNKLNAKLNALLPPDIRVKALAPVNDNFHARFSASKRHYRYILY-----NHRHYY----SPLDLEKMRAA---AKQLL 136
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599   213 GTHNFHNFTSQKGpQDPSACRYILEMYceepfVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGT 292
Cdd:TIGR00071 137 GKHDFSNFSKAKS-KSRSPIRTISDIK-----VSESGEYIIFDIIGNSFLWHMVRKIVGALVLVGRGKLPPEWVAKLLDA 210
                         250
                  ....*....|....*..
gi 70166599   293 EKVD--VPKAPGLGLVL 307
Cdd:TIGR00071 211 KKRNlaPTTAPANGLYL 227
truA PRK00021
tRNA pseudouridine(38-40) synthase TruA;
56-310 1.80e-33

tRNA pseudouridine(38-40) synthase TruA;


Pssm-ID: 234577 [Multi-domain]  Cd Length: 244  Bit Score: 125.25  E-value: 1.80e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599   56 RKIVLLMAYSGKGYHGMQR--NVgssqfKTIEDDLVSALvrsgcipenhgedmRKMSFQR----CA-RTDKGVSAAGQVV 128
Cdd:PRK00021   2 MRIALTIEYDGTNFHGWQRqpNG-----RTVQGELEKAL--------------SKLAGEPvrviGAgRTDAGVHALGQVA 62
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599  129 SL---KVWLIDDILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPT--FAFAHKDRDVQDETYRLSAETLQQ 203
Cdd:PRK00021  63 HFdtpAPRPPEKWRRALNALLPDDIAVLWAEEVPDDFHARFSAKARRYRYRIYNrpARPPFLRGYVWHYPYPLDVDAMNE 142
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599  204 VNRLLacyKGTHNFHNFTSqKGPQDPSACRYILEMYCEepfvREGlEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPE 283
Cdd:PRK00021 143 AAQYL---LGEHDFTSFRA-SGCQSKSPVRTIYEADVT----REG-DFIVFDISANGFLHNMVRNIVGTLLEVGKGKRPP 213
                        250       260
                 ....*....|....*....|....*....
gi 70166599  284 SVLERSWGTEKVD--VPKAPGLGLVLERV 310
Cdd:PRK00021 214 EDIKELLEAKDRTlaGPTAPAEGLYLVEV 242
TruA COG0101
tRNA U38,U39,U40 pseudouridine synthase TruA [Translation, ribosomal structure and biogenesis]; ...
56-313 6.27e-32

tRNA U38,U39,U40 pseudouridine synthase TruA [Translation, ribosomal structure and biogenesis]; tRNA U38,U39,U40 pseudouridine synthase TruA is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439871 [Multi-domain]  Cd Length: 250  Bit Score: 120.98  E-value: 6.27e-32
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599  56 RKIVLLMAYSGKGYHGMQR--NVgssqfKTIEDDLVSALvrsgcipenhgedmRKMSFQR----CA-RTDKGVSAAGQVV 128
Cdd:COG0101   2 MRIKLTIEYDGTNFHGWQRqpNG-----RTVQGELEKAL--------------SKLLGEPvrviGAgRTDAGVHALGQVA 62
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599 129 SLKV---WLIDDILEKINSHLPSHIRILGLKRVTGGFNSknRCDA--RTYCYLLptfaFAHKDRDVQDETY------RLS 197
Cdd:COG0101  63 HFDTpspIPPERLVRALNALLPPDIAVLWAEEVPDDFHA--RFSAksRRYRYRI----YNRPVRSPFLRGYvwhvprPLD 136
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599 198 AETLQQVNRLLacyKGTHNFHNFTSqKGPQDPSACRYILEMYCEepfvREGlEFAVIRVKGQSFMMHQIRKMVGLVVAIV 277
Cdd:COG0101 137 VEAMNEAAQLL---LGEHDFSSFRA-AGCQAKSTVRTIYRAEVE----REG-DLIVFEIEANGFLHNMVRNIVGTLVEVG 207
                       250       260       270       280
                ....*....|....*....|....*....|....*....|...
gi 70166599 278 KG-YAPESVLE------RSWGtekvdVPKAPGLGLVLERVHFE 313
Cdd:COG0101 208 RGkLSPEWIKEileakdRTRA-----GPTAPAHGLYLVEVDYP 245
PseudoU_synth_1 pfam01416
tRNA pseudouridine synthase; Involved in the formation of pseudouridine at the anticodon stem ...
211-313 1.39e-15

tRNA pseudouridine synthase; Involved in the formation of pseudouridine at the anticodon stem and loop of transfer-RNAs Pseudouridine is an isomer of uridine (5-(beta-D-ribofuranosyl) uracil, and id the most abundant modified nucleoside found in all cellular RNAs. The TruA-like proteins also exhibit a conserved sequence with a strictly conserved aspartic acid, likely involved in catalysis.


Pssm-ID: 460204 [Multi-domain]  Cd Length: 108  Bit Score: 72.18  E-value: 1.39e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599   211 YKGTHNFHNFTSQKgPQDPSACRYILEMYCEEPFVREGlEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSW 290
Cdd:pfam01416   5 YVGTHDFGNFCKQD-QPKKNTVRTILEAAVSRVGGEDG-DLIVFEVRGSGFLDHMVRAMVGVLFLVGQGKEPPEWIAELL 82
                          90       100
                  ....*....|....*....|....*.
gi 70166599   291 GTE---KVDVPKAPGLGLVLERVHFE 313
Cdd:pfam01416  83 NAKdprKIAGPTAPPVGLYLFHVRYP 108
 
Name Accession Description Interval E-value
PseudoU_synth_PUS1_PUS2 cd02568
Pseudouridine synthase, PUS1/ PUS2 like; This group consists of eukaryotic pseudouridine ...
60-312 3.06e-131

Pseudouridine synthase, PUS1/ PUS2 like; This group consists of eukaryotic pseudouridine synthases similar to Saccharomyces cerevisiae Pus1p, S. cerevisiae Pus2p, Caenorhabditis elegans Pus1p and human PUS1. Pseudouridine synthases catalyze the isomerization of specific uridines in an RNA molecule to pseudouridines (5-ribosyluracil, psi). No cofactors are required. S. cerevisiae Pus1p catalyzes the formation of psi34 and psi36 in the intron-containing tRNAIle, psi35 in the intron-containing tRNATyr, psi27 and/or psi28 in several yeast cytoplasmic tRNAs and, psi44 in U2 small nuclear RNA (U2 snRNA). The presence of the intron is required for the formation of psi 34, 35 and 36. In addition S. cerevisiae PUS1 makes are psi 26, 65 and 67. C. elegans Pus1p does not modify psi44 in U2 snRNA. Mouse Pus1p makes psi27/28 in pre- tRNASer , tRNAVal and tRNAIle, psi 34/36 in tRNAIle and, psi 32 and potentially 67 in tRNAVal. Psi44 in U2 snRNA and psi32 in tRNAs are highly phylogenetically conserved. Psi 26,27,28,34,35,36,65 and 67 in tRNAs are less highly conserved. Mouse Pus1p regulates nuclear receptor activity through pseudouridylation of Steroid Receptor RNA Activator. Missense mutation in human PUS1 causes mitochondrial myopathy and sideroblastic anemia (MLASA).


Pssm-ID: 211335 [Multi-domain]  Cd Length: 245  Bit Score: 376.57  E-value: 3.06e-131
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599  60 LLMAYSGKGYHGMQRNVGSsqFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLID--- 136
Cdd:cd02568   1 LLFGYCGTGYHGMQYNPGA--YKTIEGELERALFKAGAISESNAGDPKKIGFSRAARTDKGVHAARNVVSLKVIIDDpeg 78
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599 137 -----DILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAFahkdrdvqdetyrlsaETLQQVNRLLACY 211
Cdd:cd02568  79 lgileDLVEKLNSHLPSDIRVFGITRVTKSFNARKACDSRTYEYLLPTFAL----------------ETLQRFNEILKEY 142
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599 212 KGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVR-EGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSW 290
Cdd:cd02568 143 VGTHNFHNFTVKKKFEDPSANRFIKSFYVSEPFVIeEGLEWISIKIHGQSFMLHQIRKMIGLAIAIVRGGAPESLIELSF 222
                       250       260
                ....*....|....*....|...
gi 70166599 291 GTEKVD-VPKAPGLGLVLERVHF 312
Cdd:cd02568 223 NKDKIIiIPLAPGLGLLLERPHF 245
PseudoU_synth_TruA_like cd00497
Pseudouridine synthase, TruA family; This group consists of eukaryotic, bacterial and archeal ...
63-312 8.21e-46

Pseudouridine synthase, TruA family; This group consists of eukaryotic, bacterial and archeal pseudouridine synthases similar to Escherichia coli TruA, Saccharomyces cerevisiae Pus1p, S. cerevisiae Pus3p Caenorhabditis elegans Pus1p and human PUS1. Pseudouridine synthases catalyze the isomerization of specific uridines in an RNA molecule to pseudouridines (5-ribosyluracil, psi). No cofactors are required. S. cerevisiae PUS1 catalyzes the formation of psi34 and psi36 in the intron containing tRNAIle, psi35 in the intron containing tRNATyr, psi27 and/or psi28 in several yeast cytoplasmic tRNAs and, psi44 in U2 small nuclear RNA (U2 snRNA). The presence of the intron is required for the formation of psi 34, 35 and 36. In addition S. cerevisiae PUS1 makes psi 26, 65 and 67. C. elegans Pus1p does not modify psi44 in U2 snRNA. S. cerevisiae Pus3p makes psi38 and psi39 in tRNAs. Psi44 in U2 snRNA and, psi38 and psi39 in tRNAs are highly phylogenetically conserved. Psi 26,27,28,34,35,36,65 and 67 in tRNAs are less highly conserved. Mouse Pus1p regulates nuclear receptor activity through pseudouridylation of Steroid Receptor RNA Activator. Missense mutation in human PUS1 causes mitochondrial myopathy and sideroblastic anemia (MLASA).


Pssm-ID: 211322 [Multi-domain]  Cd Length: 215  Bit Score: 156.78  E-value: 8.21e-46
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599  63 AYSGKGYHGMQRnvgSSQFKTIEDDLVSALVRSGCIPENhgedmrkmsFQRCARTDKGVSAAGQVVSLKV--WLIDDIle 140
Cdd:cd00497   2 GYDGTKYHGFQR---QNDVPTVEGELIIALLKAGNIPYF---------IKAAARTDRGVSALGQVVAIETerRLTPEA-- 67
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599 141 kINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAfahkdrdvqdetyrlsaETLQQVNRLLACYKGTHNFHNF 220
Cdd:cd00497  68 -LNGILPGDIRVFAVHSVPPDFHAPRYCDHRTYRYYIPSFP-----------------LDDERLKSAASRFLGTHDFTNF 129
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599 221 TSQKGPqdpSACRYILEMYCEEPFvreglEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTEKVDVPK- 299
Cdd:cd00497 130 SKKDTR---NTVRTIISIECKDLN-----PFVVVEFKAKSFLWHQVRRMVGFLMLVGEGLHSPSSVSRLLAGPAPPIPMv 201
                       250
                ....*....|....
gi 70166599 300 -APGLGLVLERVHF 312
Cdd:cd00497 202 pAPAEGLLLVDVKY 215
hisT_truA TIGR00071
tRNA pseudouridine(38-40) synthase; Members of this family are the tRNA modification enzyme ...
56-307 3.98e-44

tRNA pseudouridine(38-40) synthase; Members of this family are the tRNA modification enzyme TruA, tRNA pseudouridine(38-40) synthase. In a few species (e.g. Bacillus anthracis), TruA is represented by two paralogs. [Protein synthesis, tRNA and rRNA base modification]


Pssm-ID: 272889 [Multi-domain]  Cd Length: 227  Bit Score: 152.85  E-value: 3.98e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599    56 RKIVLLMAYSGKGYHGMQRNVGssqFKTIEDDLVSALVRSGcipenhgedMRKMSFQRCARTDKGVSAAGQVVSLKV--- 132
Cdd:TIGR00071   1 RKIALKIAYDGSNYHGWQRQPN---KRTVQGELEKALEAIG---------KKKITIMSAGRTDKGVHAMGQVISFDTpke 68
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599   133 WLIDDILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPtfafaHKDRDVqdetYRLSAETLQQVnrlLACYK 212
Cdd:TIGR00071  69 IPDNKLNAKLNALLPPDIRVKALAPVNDNFHARFSASKRHYRYILY-----NHRHYY----SPLDLEKMRAA---AKQLL 136
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599   213 GTHNFHNFTSQKGpQDPSACRYILEMYceepfVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGT 292
Cdd:TIGR00071 137 GKHDFSNFSKAKS-KSRSPIRTISDIK-----VSESGEYIIFDIIGNSFLWHMVRKIVGALVLVGRGKLPPEWVAKLLDA 210
                         250
                  ....*....|....*..
gi 70166599   293 EKVD--VPKAPGLGLVL 307
Cdd:TIGR00071 211 KKRNlaPTTAPANGLYL 227
truA PRK00021
tRNA pseudouridine(38-40) synthase TruA;
56-310 1.80e-33

tRNA pseudouridine(38-40) synthase TruA;


Pssm-ID: 234577 [Multi-domain]  Cd Length: 244  Bit Score: 125.25  E-value: 1.80e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599   56 RKIVLLMAYSGKGYHGMQR--NVgssqfKTIEDDLVSALvrsgcipenhgedmRKMSFQR----CA-RTDKGVSAAGQVV 128
Cdd:PRK00021   2 MRIALTIEYDGTNFHGWQRqpNG-----RTVQGELEKAL--------------SKLAGEPvrviGAgRTDAGVHALGQVA 62
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599  129 SL---KVWLIDDILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPT--FAFAHKDRDVQDETYRLSAETLQQ 203
Cdd:PRK00021  63 HFdtpAPRPPEKWRRALNALLPDDIAVLWAEEVPDDFHARFSAKARRYRYRIYNrpARPPFLRGYVWHYPYPLDVDAMNE 142
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599  204 VNRLLacyKGTHNFHNFTSqKGPQDPSACRYILEMYCEepfvREGlEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPE 283
Cdd:PRK00021 143 AAQYL---LGEHDFTSFRA-SGCQSKSPVRTIYEADVT----REG-DFIVFDISANGFLHNMVRNIVGTLLEVGKGKRPP 213
                        250       260
                 ....*....|....*....|....*....
gi 70166599  284 SVLERSWGTEKVD--VPKAPGLGLVLERV 310
Cdd:PRK00021 214 EDIKELLEAKDRTlaGPTAPAEGLYLVEV 242
TruA COG0101
tRNA U38,U39,U40 pseudouridine synthase TruA [Translation, ribosomal structure and biogenesis]; ...
56-313 6.27e-32

tRNA U38,U39,U40 pseudouridine synthase TruA [Translation, ribosomal structure and biogenesis]; tRNA U38,U39,U40 pseudouridine synthase TruA is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 439871 [Multi-domain]  Cd Length: 250  Bit Score: 120.98  E-value: 6.27e-32
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599  56 RKIVLLMAYSGKGYHGMQR--NVgssqfKTIEDDLVSALvrsgcipenhgedmRKMSFQR----CA-RTDKGVSAAGQVV 128
Cdd:COG0101   2 MRIKLTIEYDGTNFHGWQRqpNG-----RTVQGELEKAL--------------SKLLGEPvrviGAgRTDAGVHALGQVA 62
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599 129 SLKV---WLIDDILEKINSHLPSHIRILGLKRVTGGFNSknRCDA--RTYCYLLptfaFAHKDRDVQDETY------RLS 197
Cdd:COG0101  63 HFDTpspIPPERLVRALNALLPPDIAVLWAEEVPDDFHA--RFSAksRRYRYRI----YNRPVRSPFLRGYvwhvprPLD 136
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599 198 AETLQQVNRLLacyKGTHNFHNFTSqKGPQDPSACRYILEMYCEepfvREGlEFAVIRVKGQSFMMHQIRKMVGLVVAIV 277
Cdd:COG0101 137 VEAMNEAAQLL---LGEHDFSSFRA-AGCQAKSTVRTIYRAEVE----REG-DLIVFEIEANGFLHNMVRNIVGTLVEVG 207
                       250       260       270       280
                ....*....|....*....|....*....|....*....|...
gi 70166599 278 KG-YAPESVLE------RSWGtekvdVPKAPGLGLVLERVHFE 313
Cdd:COG0101 208 RGkLSPEWIKEileakdRTRA-----GPTAPAHGLYLVEVDYP 245
PLN03078 PLN03078
Putative tRNA pseudouridine synthase; Provisional
37-313 7.86e-32

Putative tRNA pseudouridine synthase; Provisional


Pssm-ID: 215562 [Multi-domain]  Cd Length: 513  Bit Score: 126.17  E-value: 7.86e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599   37 PAKKLKSGGDEERREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCAR 116
Cdd:PLN03078  52 PLTDNSDQGGGMKWESARKKKVVLRVGYVGTDYRGLQKQRDLSSLSTIEGELETAIFKAGGIRESNYGNLHKIGWARSSR 131
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599  117 TDKGVSAAGQVVSLKVWLIDDILEK----------INSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAFAHKD 186
Cdd:PLN03078 132 TDKGVHSLATMISLKMEIPENAWKDdpdgialakfINSHLPDNIRVFSILPAQRSFDPRRECDLRKYSYLLPAEVIGIKS 211
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599  187 RDVQDETyrlsAETLQQVNRLLACYKGTHNFHNFTSQ------------------------------------------- 223
Cdd:PLN03078 212 GFSSEEI----DEHISEFNSILNGFEGEHPFHNYTARskyrkklpgkhkqrngavsrraksskemssseseenhgeisee 287
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599  224 -------------------KGPQDPSACRYILEMYCEEPFVRE----------------------GLEFAVIRVKGQSFM 262
Cdd:PLN03078 288 deedlsfssipsgssdeneDILKFQSSQVQIRARWLHEPDETDrisashfrkifrcscgklekslGFDFVELSIWGESFM 367
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|..
gi 70166599  263 MHQIRKMVGLVVAIVKGYAPESVLERSWGT-EKVDVPKAPGLGLVLERVHFE 313
Cdd:PLN03078 368 LHQIRKMVGTAVAVKRELLPRDIIRLSLTKfSRIVLPLAPSEVLILRGNSFS 419
PseudoU_synth_EcTruA cd02570
Eukaryotic and bacterial pseudouridine synthases similar to E. coli TruA; This group consists ...
60-310 1.00e-30

Eukaryotic and bacterial pseudouridine synthases similar to E. coli TruA; This group consists of eukaryotic and bacterial pseudouridine synthases similar to E. coli TruA, Pseudomonas aeruginosa truA and human pseudouridine synthase-like 1 (PUSL1). Pseudouridine synthases catalyze the isomerization of specific uridines in an RNA molecule to pseudouridines (5-ribosyluracil, psi). No cofactors are required. E. coli TruA makes psi38/39 and/or 40 in tRNA. psi38 and psi39 in tRNAs are highly phylogenetically conserved. P. aeruginosa truA is required for induction of type III secretory genes and may act through modifying tRNAs critical for the expression of type III genes or their regulators.


Pssm-ID: 211337 [Multi-domain]  Cd Length: 239  Bit Score: 117.57  E-value: 1.00e-30
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599  60 LLMAYSGKGYHGMQRNVGSsqfKTIEDDLVSALvrsgcipenhgedmRKMSFQR----CA-RTDKGVSAAGQVVSLKV-- 132
Cdd:cd02570   1 LTIEYDGTNFSGWQRQPNG---RTVQGELEKAL--------------SKIAGEPvrviGAgRTDAGVHALGQVAHFDTps 63
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599 133 -WLIDDILEKINSHLPSHIRILGLKRVTGGFNSknRCDA--RTYCYLLptfaFAHKDRDVQDETY------RLSAETLQQ 203
Cdd:cd02570  64 eIPLEKLIKALNSLLPPDIRVLSAEEVPDDFHA--RFSAksRTYRYRI----LNRPVPSPFLRRYvwhvprPLDIEAMQE 137
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599 204 VNRLLacyKGTHNFHNFTSQkGPQDPSACRYILEmyCEepfVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPE 283
Cdd:cd02570 138 AAKLL---LGTHDFSSFRAA-GCQSKSTVRTIYR--AD---VYREGDLIVFEIRANGFLYHMVRNIVGTLLEVGRGKLSP 208
                       250       260
                ....*....|....*....|....*....
gi 70166599 284 SVLERSW--GTEKVDVPKAPGLGLVLERV 310
Cdd:cd02570 209 EDIKEILeaKDRTAAGPTAPAHGLYLVKV 237
PseudoU_synth_ScPus3 cd02569
Pseudouridine synthase, Saccharomyces cerevisiae Pus3 like; This group consists of eukaryotic ...
60-312 2.53e-28

Pseudouridine synthase, Saccharomyces cerevisiae Pus3 like; This group consists of eukaryotic pseudouridine synthases similar to S. cerevisiae Pus3p, mouse Pus3p and, human PUS2. Pseudouridine synthases catalyze the isomerization of specific uridines in an RNA molecule to pseudouridines (5-ribosyluracil, psi). No cofactors are required. S. cerevisiae Pus3p makes psi38 and psi39 in tRNAs. Mouse Pus3p has been shown to makes psi38 and, possibly also psi 39, in tRNAs. Psi38 and psi39 are highly conserved in tRNAs from eubacteria, archea and eukarya.


Pssm-ID: 211336 [Multi-domain]  Cd Length: 256  Bit Score: 111.61  E-value: 2.53e-28
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599  60 LLMAYSGKGYHGMQRNVGSSQfkTIEDDLVSALVRSGCIpenhgEDMRKMSFQRCARTDKGVSAAGQVVSLKV------- 132
Cdd:cd02569   1 LRFAYLGWNYNGFAVQEETTN--TVEETLFEALEKTRLI-----EDRQTSNYSRCGRTDKGVSAFGQVISLDVrsnlkpe 73
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599 133 WLID--------------DILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLlptfaFAHKDRDVqdetyrlsa 198
Cdd:cd02569  74 DGLDpstdvkstadeeelPYCKILNRVLPPDIRILAWAPVPPDFSARFSCVSRTYRYF-----FPKGDLDI--------- 139
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599 199 ETLQQVNRLlacYKGTHNFHNFTSQKGPQDPSAC-RYILEMYCEE-PFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAI 276
Cdd:cd02569 140 ELMRKAAKL---LLGEHDFRNFCKMDVANQVTNYvRRVLSAEVEPvDQHPDGDGLYYFEVRGSAFLWHQVRCMMAVLFLI 216
                       250       260       270       280
                ....*....|....*....|....*....|....*....|..
gi 70166599 277 VKGYAPESVLERSWGTEKvdVPK------APGLGLVLERVHF 312
Cdd:cd02569 217 GQGLEPPSVISQLLDVEK--NPRkpqytmASEVPLVLYDCGF 256
PseudoU_synth_TruA_Archea cd02866
Archeal pseudouridine synthases; This group consists of archeal pseudouridine synthases. ...
60-307 1.44e-24

Archeal pseudouridine synthases; This group consists of archeal pseudouridine synthases.Pseudouridine synthases catalyze the isomerization of specific uridines in an RNA molecule to pseudouridines (5-ribosyluracil, psi). No cofactors are required. This group of proteins make Psedouridine in tRNAs.


Pssm-ID: 211343 [Multi-domain]  Cd Length: 219  Bit Score: 100.53  E-value: 1.44e-24
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599  60 LLMAYSGKGYHGMQRNvgsSQFKTIEDDLVSALVRSGCIPEnhgedmrKMSFQRCARTDKGVSAAGQVVSlkVWLIDD-I 138
Cdd:cd02866   1 LKIAYDGTNFHGFQRQ---PDVRTVEGELIKALEELGIIES-------RARLYSAGRTDRGVHALGNVVV--FETEKEpI 68
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599 139 LEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPtfafahkdrdvqdETYRLSAetLQQVNRLLacyKGTHNFH 218
Cdd:cd02866  69 PPMINAKLPKDIWVLAGAKVPEDFDPRRWAHRKYYRYNLG-------------SDYDVEA--MKEAAKKL---IGTHDFS 130
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599 219 NFTSQKGPQDPsacryILEMY-CEepfVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTE--KV 295
Cdd:cd02866 131 NFSKRDGRKDP-----VRTIErIE---ISENGEFITIDVVGESFLWNMVRRIVGALSEVGKGKRENEWVEKLLDGEfrPE 202
                       250
                ....*....|..
gi 70166599 296 DVPKAPGLGLVL 307
Cdd:cd02866 203 GVPPAPPEGLIL 214
PseudoU_synth_1 pfam01416
tRNA pseudouridine synthase; Involved in the formation of pseudouridine at the anticodon stem ...
211-313 1.39e-15

tRNA pseudouridine synthase; Involved in the formation of pseudouridine at the anticodon stem and loop of transfer-RNAs Pseudouridine is an isomer of uridine (5-(beta-D-ribofuranosyl) uracil, and id the most abundant modified nucleoside found in all cellular RNAs. The TruA-like proteins also exhibit a conserved sequence with a strictly conserved aspartic acid, likely involved in catalysis.


Pssm-ID: 460204 [Multi-domain]  Cd Length: 108  Bit Score: 72.18  E-value: 1.39e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599   211 YKGTHNFHNFTSQKgPQDPSACRYILEMYCEEPFVREGlEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSW 290
Cdd:pfam01416   5 YVGTHDFGNFCKQD-QPKKNTVRTILEAAVSRVGGEDG-DLIVFEVRGSGFLDHMVRAMVGVLFLVGQGKEPPEWIAELL 82
                          90       100
                  ....*....|....*....|....*.
gi 70166599   291 GTE---KVDVPKAPGLGLVLERVHFE 313
Cdd:pfam01416  83 NAKdprKIAGPTAPPVGLYLFHVRYP 108
PRK14587 PRK14587
tRNA pseudouridine synthase ACD; Provisional
113-307 1.32e-10

tRNA pseudouridine synthase ACD; Provisional


Pssm-ID: 173051  Cd Length: 256  Bit Score: 61.38  E-value: 1.32e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599  113 RCARTDKGVSAAGQVVSLKVWLiddILEKINSHLPSHIRILGLKRVTGGFNSKnRCDARTYCYLLPtfafaHKDRDVqdE 192
Cdd:PRK14587  38 RGSRTDPGVSAVGNVVMTSQKL---PLGYVNSKLPRGVWAWAVAEVPEGFNPR-RAKRRRYLYVAP-----HWGEDV--E 106
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70166599  193 TYRLSAETLqqvnrllacyKGTHNFHNFTSQKGPQDPSACRYILEMYCEepfVREGLEFavIRVKGQSFMMHQIRKMVGL 272
Cdd:PRK14587 107 AMREAAELL----------AGTHDYSSFIQRRGEKATPTVTTVYEIGVE---LRGDLIY--LYFVGRGFRNKMIRKMAWA 171
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|.
gi 70166599  273 VVAIVKGyapesVLERSWGTEKVD------VPKAPGLGLVL 307
Cdd:PRK14587 172 ILAAGRG-----VLSRRDIAELLErprpgaVPSAPAEGLVL 207
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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