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Conserved domains on  [gi|28827786|ref|NP_789778|]
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multiple coagulation factor deficiency protein 2 homolog precursor [Mus musculus]

Protein Classification

EF-hand domain-containing protein( domain architecture ID 12144783)

EF-hand (EFh) domain-containing protein may be involved in binding intracellular calcium and in calcium signal transduction

CATH:  1.10.238.10
Gene Ontology:  GO:0005509
PubMed:  2479149|10191494
SCOP:  3001983

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
EF-hand_7 pfam13499
EF-hand domain pair;
76-142 1.24e-05

EF-hand domain pair;


:

Pssm-ID: 463900 [Multi-domain]  Cd Length: 67  Bit Score: 40.70  E-value: 1.24e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 28827786    76 FKMHDYDGNSLLDGLELSIAITHVHKEEgseqapVMSEDElvsiIDGVLRDDDKNNDGYIDYAEFAK 142
Cdd:pfam13499   8 FKLLDSDGDGYLDVEELKKLLRKLEEGE------PLSDEE----VEELFKEFDLDKDGRISFEEFLE 64
 
Name Accession Description Interval E-value
EF-hand_7 pfam13499
EF-hand domain pair;
76-142 1.24e-05

EF-hand domain pair;


Pssm-ID: 463900 [Multi-domain]  Cd Length: 67  Bit Score: 40.70  E-value: 1.24e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 28827786    76 FKMHDYDGNSLLDGLELSIAITHVHKEEgseqapVMSEDElvsiIDGVLRDDDKNNDGYIDYAEFAK 142
Cdd:pfam13499   8 FKLLDSDGDGYLDVEELKKLLRKLEEGE------PLSDEE----VEELFKEFDLDKDGRISFEEFLE 64
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 76-142 2.45e-05

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 39.84  E-value: 2.45e-05
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 28827786  76 FKMHDYDGNSLLDGLELSIAIthvhkeegseqaPVMSEDELVSIIDGVLRDDDKNNDGYIDYAEFAK 142
Cdd:cd00051   6 FRLFDKDGDGTISADELKAAL------------KSLGEGLSEEEIDEMIREVDKDGDGKIDFEEFLE 60
PTZ00184 PTZ00184
calmodulin; Provisional
 57-144 2.53e-03

calmodulin; Provisional


Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 35.89  E-value: 2.53e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28827786   57 GVIDQPE---------AEMSPQELQLHYFKMHDYDGNSLLDGLELSIAITHVHKEegseqapvMSEDElvsiIDGVLRDD 127
Cdd:PTZ00184  62 GTIDFPEfltlmarkmKDTDSEEEIKEAFKVFDRDGNGFISAAELRHVMTNLGEK--------LTDEE----VDEMIREA 129

                         90
                 ....*....|....*..
gi 28827786  128 DKNNDGYIDYAEFAKSL 144
Cdd:PTZ00184 130 DVDGDGQINYEEFVKMM 146
 
Name Accession Description Interval E-value
EF-hand_7 pfam13499
EF-hand domain pair;
76-142 1.24e-05

EF-hand domain pair;


Pssm-ID: 463900 [Multi-domain]  Cd Length: 67  Bit Score: 40.70  E-value: 1.24e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 28827786    76 FKMHDYDGNSLLDGLELSIAITHVHKEEgseqapVMSEDElvsiIDGVLRDDDKNNDGYIDYAEFAK 142
Cdd:pfam13499   8 FKLLDSDGDGYLDVEELKKLLRKLEEGE------PLSDEE----VEELFKEFDLDKDGRISFEEFLE 64
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 76-142 2.45e-05

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 39.84  E-value: 2.45e-05
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 28827786  76 FKMHDYDGNSLLDGLELSIAIthvhkeegseqaPVMSEDELVSIIDGVLRDDDKNNDGYIDYAEFAK 142
Cdd:cd00051   6 FRLFDKDGDGTISADELKAAL------------KSLGEGLSEEEIDEMIREVDKDGDGKIDFEEFLE 60
EFh_HEF cd15902
EF-hand, calcium binding motif, found in the hexa-EF hand proteins family; The hexa-EF hand ...
 76-144 7.46e-04

EF-hand, calcium binding motif, found in the hexa-EF hand proteins family; The hexa-EF hand proteins family, also named the calbindin sub-family, contains a group of six EF-hand Ca2+-binding proteins, including calretinin (CR, also termed 29 kDa calbindin), calbindin D28K (CB, also termed vitamin D-dependent calcium-binding protein, avian-type), and secretagogin (SCGN). CR is a cytosolic hexa-EF-hand calcium-binding protein predominantly expressed in a variety of normal and tumorigenic t-specific neurons of the central and peripheral nervous system. It is a multifunctional protein implicated in many biological processes, including cell proliferation, differentiation, and cell death. CB is highly expressed in brain tissue. It is a strong calcium-binding and buffering protein responsible for preventing a neuronal death as well as maintaining and controlling calcium homeostasis. SCGN is a six EF-hand calcium-binding protein expressed in neuroendocrine, pancreatic endocrine and retinal cells. It plays a crucial role in cell apoptosis, receptor signaling and differentiation. It is also involved in vesicle secretion through binding to various proteins, including interacts with SNAP25, SNAP23, DOC2alpha, ARFGAP2, rootletin, KIF5B, beta-tubulin, DDAH-2, ATP-synthase and myeloid leukemia factor 2. SCGN functions as a Ca2+ sensor/coincidence detector modulating vesicular exocytosis of neurotransmitters, neuropeptides or hormones. Although the family members share a significant amount of secondary sequence homology, they display altered structural and biochemical characteristics, and operate in distinct fashions. CB contains six EF-hand motifs in a single globular domain, where EF-hands 1, 3, 4, 5 bind four calcium ions. CR contains six EF-hand motifs within two independent domains, CR I-II and CR III-VI. They harbor two and four EF-hand motifs, respectively. The first 5 EF-hand motifs are capable of binding calcium ions, while the EF-hand 6 is inactive. SCGN consists of the three globular domains each of which contains a pair of EF-hand motifs. Human SCGN simultaneously binds four calcium ions through its EF-hands 3, 4, 5 and 6 in one high affinity and three low affinity calcium-binding sites. In contrast, SCGNs in other lower eukaryotes, such as D. rerio, X. laevis, M. domestica, G. gallus, O. anatinus, are fully competent in terms of six calcium-binding.


Pssm-ID: 320075 [Multi-domain]  Cd Length: 254  Bit Score: 38.10  E-value: 7.46e-04
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 28827786  76 FKMHDYDGNSLLDGLELSIAITHVHKeegSEQAPVMSEDELVSIIDGVLRDDDKNNDGYIDYAEFAKSL 144
Cdd:cd15902   5 WMHFDADGNGYIEGKELDSFLRELLK---ALNGKDKTDDEVAEKKKEFMEKYDENEDGKIEIRELANIL 70
PTZ00184 PTZ00184
calmodulin; Provisional
 57-144 2.53e-03

calmodulin; Provisional


Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 35.89  E-value: 2.53e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28827786   57 GVIDQPE---------AEMSPQELQLHYFKMHDYDGNSLLDGLELSIAITHVHKEegseqapvMSEDElvsiIDGVLRDD 127
Cdd:PTZ00184  62 GTIDFPEfltlmarkmKDTDSEEEIKEAFKVFDRDGNGFISAAELRHVMTNLGEK--------LTDEE----VDEMIREA 129

                         90
                 ....*....|....*..
gi 28827786  128 DKNNDGYIDYAEFAKSL 144
Cdd:PTZ00184 130 DVDGDGQINYEEFVKMM 146
EFh_CREC cd15899
EF-hand, calcium binding motif, found in CREC-EF hand family; The CREC (Cab45/reticulocalbin ...
 56-140 3.73e-03

EF-hand, calcium binding motif, found in CREC-EF hand family; The CREC (Cab45/reticulocalbin/ERC45/calumenin)-EF hand family contains a group of six EF-hand, low-affinity Ca2+-binding proteins, including reticulocalbin (RCN-1), ER Ca2+-binding protein of 55 kDa (ERC-55, also known as TCBP-49 or E6BP), reticulocalbin-3 (RCN-3), Ca2+-binding protein of 45 kDa (Cab45 and its splice variant Cab45b), and calumenin ( also known as crocalbin or CBP-50). The proteins are not only localized in various parts of the secretory pathway, but also found in the cytosolic compartment and at the cell surface. They interact with different ligands or proteins and have been implicated in the secretory process, chaperone activity, signal transduction as well as in a large variety of disease processes.


Pssm-ID: 320021 [Multi-domain]  Cd Length: 267  Bit Score: 36.27  E-value: 3.73e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28827786  56 EGVIDQPEAEMSPQELQLH---YFKMHDYDGNSLLDGLELsiaITHVHKEEGSEQAPVMSEDelvsiidgVLRDDDKNND 132
Cdd:cd15899 106 ENVADNIKEDEEYKKLLLKdkkRFEAADQDGDLILTLEEF---LAFLHPEESPYMLDFVIKE--------TLEDLDKNGD 174


                ....*...
gi 28827786 133 GYIDYAEF 140
Cdd:cd15899 175 GFISLEEF 182
EFh_HEF_CBN cd16179
EF-hand, calcium binding motif, found in Drosophila melanogaster calbindin-32 (CBN) and ...
 74-144 4.04e-03

EF-hand, calcium binding motif, found in Drosophila melanogaster calbindin-32 (CBN) and similar proteins; CBN, the product of the cbn gene, is a Drosophila homolog to vertebrate neuronal six EF-hand calcium binding proteins. It is expressed through most of ontogenesis with a selective distribution in the nervous system and in a few small adult thoracic muscles. Its precise biological role remains unclear. CBN contains six EF-hand motifs, but some of them may not bind calcium ions due to the lack of key residues.


Pssm-ID: 320079 [Multi-domain]  Cd Length: 261  Bit Score: 35.85  E-value: 4.04e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28827786  74 HYfkmhDYDGNSLLDGLEL---------SIAITHVHKEegseqapVMSEDELVSIIDGVLRDDDKNNDGYIDYAEFAKSL 144
Cdd:cd16179   7 HY----DTDGNGYIEGTELdgflrefvsSVNPEDVGPE-------VVSETALEELKEEFMEAYDENQDGRIDIRELAQLL 75
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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