Conserved Protein Domain Family
PIPKc_PIP5K1A_like
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cd17306: PIPKc_PIP5K1A_like 
Phosphatidylinositol phosphate kinase (PIPK) catalytic domain found in phosphatidylinositol 4-phosphate 5-kinase type-1 alpha (PIP5K1alpha) and similar proteins
PIP5K1alpha (EC 2.7.1.68), also termed PIP5K1A, or PtdIns(4)P-5-kinase 1 alpha, or 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha, or PIPKI-alpha, catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). It mediates extracellular calcium-induced keratinocyte differentiation. Unlike other type I phosphatidylinositol-4-phosphate 5-kinase (PIPKI) isoforms, PIP5K1alpha regulates directed cell migration by modulating Rac1 plasma membrane targeting and activation. This function is independent of its catalytic activity, and requires physical interaction of PIP5K1alpha with the Rac1 polybasic domain. The family also includes testis-specific PIP5K1A and PSMD4-like protein, also known as PIP5K1A-PSMD4 or PIPSL. It has negligeable PIP5 kinase activity and binds to ubiquitinated proteins.
Links
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Statistics
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PSSM-Id: 340443
Aligned: 5 rows
Threshold Bit Score: 684.419
Created: 8-Mar-2017
Updated: 2-Oct-2020
Structure
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Aligned Rows:
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Feature 1: catalytic core residues [active site], 3 residue positions
Conserved feature residue pattern:K D DClick to see conserved feature residue pattern help
Evidence:
  • Comment:Lys proposed to bind the alpha-phosphate of ATP; mutation of this residue in PIPKI-alpha and PIPKII-alpha destroys enzyme activity.
  • Comment:1st Asp functions as a weak base
  • Comment:2nd Asp binds catalytic Mg2+ or Mn2+
  • Citation:PMID 9753329
Scroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                        
Q99755        80 TTSSALKGAIQLGITHTVGSLStKPERDVLMQDFYVVESIFFPSeGSNLTPAHHyNDFRFKTYAPVAFRYFRELFGIRPD 159 human
A2A3N6        27 TTSSALKGAIQLGITHTVGSLStKPESDVLMQDFHMVESIFFPSeGSNLTPAHHyNAFRFKTYAPVAFRYFWELFGIRPD 106 human
NP_001135912  73 TTSSALKGAIQLGITHTVGSLStKPERDVLMQDFYVVESIFFPSeGSNLTPAHHyNDFRFKTYAPVAFRYFRELFGIRPD 152 chicken
XP_006003369  76 TTSSALKGAIQLGITHTVGSLSnKPERDVLMQDFYVVESIFFPReGSNLTPAHHySDFRFKTYAPMAFRHFRELFEIRPD 155 coelacanth
NP_001233235  58 TTSSALKGAIQLGITHTVGSLStKPERDVLMQDFYVVESIFFPGeGSNLTPAHHyNDFRFKTYAPVAFRYFRELFGIRPD 137 African clawed ...

Feature 1                                          #                                             
Q99755       160 DYLYSLCsEPLIELCsSGASGSLFYVSSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQAGGKNI 239 human
A2A3N6       107 DYLYSLCsEPLIELCsSGASGSLFYVSSDDEFIVKTVRHKEAEFLQKLLPGYYINLNQNPRTLLPKFYGLYCVQTGGKNI 186 human
NP_001135912 153 DYLYSLCsEPLIELSnSGASGSLFYVSSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQAGGKNI 232 chicken
XP_006003369 156 DYLYSLCkEPLIELSnSGASGSLFYVSGDDEFIIKTVQHKEAEFLQKLLPGYYMNVKQNPRTLLPKFYGLYCVQAGGKNI 235 coelacanth
NP_001233235 138 DYLYSLCnEPLIELSnPGASGSVFYVSGDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQAGGKNI 217 African clawed ...

Feature 1                                                                                        
Q99755       240 RIVVMNNLLPRsVKMHIKYDLKGSTYKRRASqkerekplPTFKDLDFLQDiPDGLFLDaDMYNALCKTLQRDCLVLQSFK 319 human
A2A3N6       187 RIVVMNNLLPRsVKMHIKYDLKGSTYRRRASqkerekplPTFKDLDFLQDiPDGLFLDaDVHNALCKTLQRDCLVLQSFK 266 human
NP_001135912 233 RIVVMNNLLPRsVKMHLKYDLKGSTYKRRASqkerekvfPTYKDLDFMQDiPDGLFLDsDMYNALCKTLQRDCLVLQSFK 312 chicken
XP_006003369 236 RIVVMNNLLPRsVKMHLKYDLKGSTYKRRASmkerekplPTYKDLDFMQDiAEGLYLDsDMYSALCKTMQRDCLVLQSFK 315 coelacanth
NP_001233235 218 RIVVMNNLLPRsVRMHLKYDLKGSTYKRRASakerekssPTHKDLDFLQDlPDGLYLDpDMHNALCKTIQRDCLVLQSFK 297 African clawed ...

Feature 1          #                                                                             
Q99755       320 IMDYSLLMSIHNIDhaqreplssetqysvdtrr------papqkalystamesiqgeARRGGtmETDDHMGGIPARNSKG 393 human
A2A3N6       267 IMDYSLLMSIHNIDhaqreplssetqysvdtrr------papqkalystamesiqgeARRGGtmETDDHMGGIPARNSKG 340 human
NP_001135912 313 IMDYSLLVAIHNMDlaqrehvmadgtsqadtrr------paaqkalystamesiqgeARRGGtiETDDQMGGIPARNAKG 386 chicken
XP_006003369 316 IMDYSLLVGIHNIDqslrereleghqgtmdgvmsldtrkpvgqkalystpmesiqggALNGGtiETEDQMGGIPARNSKG 395 coelacanth
NP_001233235 298 IMDYSLLVGVHNMDfaqketmvvdaqsdtrrp--------aaqkalystamesiqgeARRGGaiETDDQMGGIPARNAKG 369 African clawed ...

Feature 1                  #                                                   
Q99755       394 ERLLLYIGIIDILQSYRFVKKLEHSWKALVHDGDTvSVHRPGFYAERFQRFMCNTVFKKIPL 455 human
A2A3N6       341 ERLLLYIGIIDILQSYRFVKKLEHSWKALIHDGDTvSVHRPGFYAEWFQRFMCNTVFKKIPL 402 human
NP_001135912 387 ERLLLYVGIIDVLQSYRFVKKLEHSWKALVHDGDTvSVHRPSFYAERFQRFMCNTVFKKIPL 448 chicken
XP_006003369 396 ERLLLYIGIIDILQSYRFVKKLEHSWKALVHDGDTvSVHRPGFYAERFQKFMCTTVFKKIPL 457 coelacanth
NP_001233235 370 ERLLLYIGVIDVLQSYRFMKKLEHSWKALVHDGDTvSVHRPGFYADRFQKFMCVSVFKKIPL 431 African clawed frog

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