2PGI,2Q8N,1T10,1IRI,1U0F,1KOJ,1ZZG,1GZD


Conserved Protein Domain Family
SIS_PGI_1
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cd05015: SIS_PGI_1 
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Phosphoglucose isomerase (PGI) contains two SIS (Sugar ISomerase) domains. This classification is based on the alignment of the first SIS domain. PGI is a multifunctional enzyme which as an intracellular dimer catalyzes the reversible isomerization of glucose 6-phosphate to fructose 6-phosphate. As an extracellular protein, PGI also has functions equivalent to neuroleukin (NLK), autocrine motility factor (AMF), and maturation factor (MF). Evidence suggests that PGI, NLK, AMF, and MF are closely related or identical. NLK is a neurotrophic growth factor that promotes regeneration and survival of neurons. The dimeric form of NLK has isomerase function, whereas its monomeric form carries out neurotrophic activity. AMF is a cytokine that stimulates cell migration and metastasis. MF mediates the differentiation of human myeloid leukemic HL-60 cells to terminal monocytic cells.
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Statistics
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PSSM-Id: 240146
Aligned: 124 rows
Threshold Bit Score: 128.027
Created: 28-Mar-2007
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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active sitedimer interface
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1U0F: Mus musculus phosphoglucose isomerase binds substrate glucose 6-phosphate (G6P), contacts calculated at 3.5A
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  • Structure:1U0F: Mus musculus phosphoglucose isomerase binds inhibitor erythrose 4-phosphate (E4P), contacts calculated at 3.5A
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  • Structure:1T10: Leishmania mexicana phosphoglucose isomerase binds substrate fructose-6-phosphate, contacts calculated at 3.5A
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  • Structure:1IRI: Human phosphoglucose isomerase binds inhibitor erythritol 4-phosphate, contacts calculated at 3.5A
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  • Structure:1KOJ; Oryctolagus cuniculus phosphoglucose isomerase bound to 5-phospho-D-arabinonohydroxamic acid (5PAH) inhibits enzymatic activity, contacts calculated at 3.5A
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  • Comment:catalytic activity of PGI is dependent on dimer formation; a conserved histidine from chain B is required
  • Comment:phosphohexoses and inhibitors of both PGI enzymatic and cytokine activity share similar binding sites
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                       # ##                                          
2PGI       55 KNEFSRIKQAAErirn---------hsdaLVVIGIGGSYLGARAAIEALshtfhnq---mndttqIYFAGQNISstyiSH 122 Geobacillus stearo...
1U0F_A    123 NRVLDKMKSFCQrvrsgdwkgytgksitdIINIGIGGSDLGPLMVTEALkpysk-------ggprVWFVSNIDGth-iAK 194 house mouse
Q7V7M6    115 AKEIDLIETFGSnvinglikapngkkftdVLWIGIGGSGLGPLLMIRALqnae--------qglrFHFFDNVDPdg-mSR 185 Prochlorococcus ma...
CAA23001  156 ENTLDSICAFSDdiisgkppsspegrftqILSVGIGGSALGPQFVAEALapdn--------pplkIRFIDNTDPag-iDH 226 thale cress
YP_518267  88 KETLKEILDFAEqihsgrlqgekgnpfrnILLVGVGGSILGPRFVADALassr--------dkmkAFFIDNGDPdg-iDR 158 Desulfitobacterium...
NP_485093  88 VQTLEQIEAFAEkiqtgaihppkanrftdIISIGIGGSALGPQFVAEALapef--------pplkIHFIDNTDPag-iDR 158 Nostoc sp. PCC 7120
AAL56572   66 GKSLEHSLKLWEkvntfvss--irndykhIIFNGIGGSYLGPYMLIQAMlgddynlslqkrglpsLHFLANTDSds-fST 142 Spironucleus barkh...
1ZZG_A     50 TETLREVRRYREanpw----------vedFVLIGIGGSALGPKALEAAFnes----------gvrFHYLDHVEPep-iLR 108 Thermus thermophilus
NP_280684  55 TVDVDAITAAADavad----------adtLVTVGIGGSALGAATLVDALgdd----------dldYHALDNVDPah-vTR 113 Halobacterium sp. ...
Q5UXU0     59 ETIHDAVAPVADae--------------sVITVGIGGSALGAKTITEALaed----------pgsHVVLDNVDPeh-vRR 113 Haloarcula marismo...

Feature 1                      ###  #                                                         
2PGI      123 LLDvle--gkdLSINVISKSGTTtEPAIAFRIFRDYMekkygk-eearkrIYVTTDrtkgalkkladqegyeTFVIPDNI 199 Geobacillus stearo...
1U0F_A    195 TLAsls--petSLFIIASKTFTTqETITNAETAKEWFleaakdpsavakhFVALSTntakvke--fgidpqnMLEFWDWV 270 house mouse
Q7V7M6    186 VLGnlgdalstTLVVTVSKSGATpEPHLGMEQARQRLeamgg---hwagqAVAVTMlnsqldqlaqkeswlkRFDMFDWV 262 Prochlorococcus ma...
CAA23001  227 QIAqlgpelasTLVVVISKSGGTpETRNGLLEVQKAFreagl---nfakqGVAITQenslldntariegwlaRFPMYDWV 303 thale cress
YP_518267 159 VLSrigeelpaTLCLIISKSGGTiETRNGMLEVRRAYkeagl---sfpdhAVAITQrgsqldklsqkegwlrAFPMWDWV 235 Desulfitobacterium...
NP_485093 159 ILThlrnhlasTLVLVISKSGGTpEPRNGMIEVKKAYagqnl---dfaqyAVAITStgsnldkvaqsegwlaTFPMYDWV 235 Nostoc sp. PCC 7120
AAL56572  143 LFDlld--vkkTLMVTISKSGSTaETATNTQCYLNLLtkasl---spsahCIAITIpgsnlhk--laaswlqTFEMCEPT 215 Spironucleus barkh...
1ZZG_A    109 LLRtld--prkTLVNAVSKSGSTaETLAGLAVFLKWLkahlge--dwrrhLVVTTDpkegplrafaereglkAFAIPKEV 184 Thermus thermophilus
NP_280684 114 LLDrid--lstSAVHVVSRSGTTaETLANFLVVRDAMddagv---dwtarTLVTTGddgplral-adthdlpVLDAPRGV 187 Halobacterium sp. ...
Q5UXU0    114 TLDgls--ladTAINVVSRSGTTaETLANFLVVREAYedrgv---dwterIVVTTGesgplral-adqhdlpTLPVPEGV 187 Haloarcula marismo...

Feature 1      #                
2PGI      200 GGrysvltavgLLPIAVA 217 Geobacillus stearothermophilus
1U0F_A    271 GGrysl-wsaiGLSIALH 287 house mouse
Q7V7M6    263 GGrtsitsavgLLPAALI 280 Prochlorococcus marinus str. MIT 9313
CAA23001  304 GGrtsimsavgLLPAALQ 321 thale cress
YP_518267 236 GGrtsllsavgLLSLALQ 253 Desulfitobacterium hafniense Y51
NP_485093 236 GGrtsemssvgLVPAALQ 253 Nostoc sp. PCC 7120
AAL56572  216 GGrtsicsavaMVPCAFA 233 Spironucleus barkhanus
1ZZG_A    185 GGrfsalspvgLLPLAFA 202 Thermus thermophilus
NP_280684 188 PGrfaalstvaLPAAAIA 205 Halobacterium sp. NRC-1
Q5UXU0    188 PGrfsalsavgLVPPAIL 205 Haloarcula marismortui

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