1SRA,1BMO


Conserved Protein Domain Family
SPARC_EC
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cd00252: SPARC_EC (this model, PSSM-Id:238155 is obsolete and has been replaced by 320009)
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SPARC_EC; extracellular Ca2+ binding domain (containing 2 EF-hand motifs) of SPARC and related proteins (QR1, SC1/hevin, testican and tsc-36/FRP). SPARC (BM-40) is a multifunctional glycoprotein, a matricellular protein, that functions to regulate cell-matrix interactions; binds to such proteins as collagen and vitronectin and binds to endothelial cells thus inhibiting cellular proliferation. The EC domain interacts with a follistatin-like (FS) domain which appears to stabilize Ca2+ binding. The two EF-hands interact canonically but their conserved disulfide bonds confer a tight association between the EF-hand pair and an acid/amphiphilic N-terminal helix. Proposed active form involves a Ca2+ dependent symmetric homodimerization of EC-FS modules.
Links
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Statistics
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PSSM-Id: 238155
Aligned: 11 rows
Threshold Bit Score: 145.981
Created: 4-Sep-2001
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 24 residues -Click on image for an interactive view with Cn3D
Feature 1:EF-hand Ca2+ binding loops [ion binding site]
Evidence:
  • Comment:putative endothelial cell receptor-binding site of BM-40 involves the accessible surface of the entire EF-hand pair
  • Structure:1SRA; Ca2+ binding residues
    - View structure with Cn3D
  • Citation:PMID 9233787
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                     
1SRA        2 PCLDSELTEFPLRMRDWLKNVLVTLYerdednn-lltekqKLRVKKIhenekrleagdhpvellardfeknyNMYIFPVH 80  human
1BMO_A     84 PCLDSELTEFPLRMRDWLKNVLVTLYerdednn-lltekqKLRVKKIhenekrleagdhpvellardfeknyNMYIFPVH 162 human
134273    484 ACTDFEVAQFPLRMRDWLKNILMQLYepnpkhggylnekqRSKVKKIyldekrllagdhpielllrdfkknyHMYVYPVH 563 Norway rat
464320    140 ECTEEHMAQFPERMADWLFQVMKELKkrr--------elhKLEWEELlseae----------------nddeKKHVYPVI 195 Caenorhabditis ele...
4499900   170 SCEGEDLKDFPRRMRDWLFNVMRDLAerde------ltehYMQMELEaet-------------------nnsRRWSNAAV 224 fruit fly
6678111   200 ACTDKELRNLASRLKDWFGALHEDANrvikpt---ssdgaQGRFDTSi-----------------------lPICKDSLG 253 house mouse
7300954   427 ECKPQQLTAIGNRLLDWFSVIMADSKkrr---------qhSQKSKAHf-----------------------pPACKTEAK 474 fruit fly
11995033  157 ECSEQEMDDFPRRMRDWLFNIMRDLAarhe------lsphYLKLEKEaev-------------------eqsKRWANAAI 211 Artemia franciscana
13559580   39 HCTRSELMRMGGRLVKWFKDVHAQESg------------aDHTLKLHs------------------------VPCRVEVG 82  Caenorhabditis ele...
14722352  192 ACSDLEFREVANRLRDWFKALHESGSqnkktk--tllrpeRSRFDTSi-----------------------lPICKDSLG 246 human
14740509   89 TCTGQDLADLGDRLRDWFQLLHENSKqngsas---svagpASGLDKSl-----------------------gASCKDSIG 142 human

Feature 1           ### #########                       ############                
1SRA       81 WQFGQLDQHpIDGYLSHTELAPLrapLIPMEHCTTRFFETC-DLDNDKYIALDEWAGCFgIKQKDIDKDL 149 human
1BMO_A    163 WQFGQLDQHpIDGYLSHTELAPLrapLIPMEHCTTRFFETC-DLDNDKYIALDEWAGCFgIKQKDIDKDL 231 human
134273    564 WQFNELDQHpADRILTHSELAPLrasLVPMEHCITRFFEEC-DPNKDKHITLKEWGHCFgIKEEDIDENL 632 Norway rat
464320    196 WKFCELDTKpHDKSVSHHELIPItapVIPMESCIKPFLEGC-DANNDGNISIKEWGKCLgLKEGEIQERC 264 Caenorhabditis elegans
4499900   225 WKWCDLDGD-TDRSVSRHELFPIrapLVSLEHCIAPFLESC-DSNKDHRITLVEWGACLeLDPEDLKERC 292 fruit fly
6678111   254 WMFNKLDMN-YDLLLDHSEINAIy--LDKYEPCIKPLFNSC-DSFKDGKLSNNEWCYCF-QKPAGLPCQN 318 house mouse
7300954   475 WMFGHLDLN-NDGQLSLQEMYDLe--HDQNERCIKPFIDTC-DLDTDSSINTREWCRCF-EKTDRPCAAV 539 fruit fly
11995033  212 WKFCDLDGHpHDRKVSRHELFPIkapLMALEHCISPFLNKC-DVDDDHFITLREWGKCLeIPEDELEDKC 280 Artemia franciscana
13559580   83 WMFNQWDGN-QDGKLSKAELRPIe--RGGNEACVEEFIDMCdDMVVDGSISVDEWCDCF-TFSDDLRHEP 148 Caenorhabditis elegans
14722352  247 WMFNRLDTN-YDLLLDQSELRSIy--LDKNEQCTKAFFNSC-DTYKDSLISNNEWCYCF-QRQQDPPCQT 311 human
14740509  143 WMFSKLDTS-ADLFLDQTELAAIn--LDKYEVCIRPFFNSC-DTYKDGRVSTAEWCFCF-WREKPPCLAE 207 human

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