2HGS


Conserved Protein Domain Family
eu-GS

?
cd00228: eu-GS 
Click on image for an interactive view with Cn3D
Eukaryotic Glutathione Synthetase (eu-GS); catalyses the production of glutathione from gamma-glutamylcysteine and glycine in an ATP-dependent manner. Belongs to the ATP-grasp superfamily.
Links
?
Statistics
?
PSSM-Id: 238140
Aligned: 10 rows
Threshold Bit Score: 706.828
Created: 4-Sep-2001
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:active site residues [active site]
Evidence:
  • Comment:residues involved in the 2 step peptide ligating reaction (gamma-Glu-Cys to gamma-Glu-Cys-Gly (GSH))

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
2HGS_A      6 GSLLQ-DKQQLEELARQAVDRALa---EGVLLRTsqep----tssEVVSYAPFTLFPSLVPSALLEQAYAVQMDFNLLVD 77  human
AAK77663   24 TTLLF-DQQLLQKIADDALVWASl---HGLVMGDksvqrsgtvsgVGLVHAPFALFPTLFPESQWRQACDLAPIFNELVD 99  Lotus japonicus
AAF98157   70 PPLFDyHSIDQKLLENIVYDALVwstlNCLLVGDksvqrsgtvpgVGLVHLPLCLLPGPFPESHWKQACELAPIFNELVD 149 French bean
1170037     5 KPIFDlEKLDDEFVQKLVYDALVwsslHGLVVGDksyqksgnvpgVGLMHAPIALLPTAFPEAYWKQACNVTPLFNELID 84  thale cress
T04336     73 KPIVDpHDIDSKLVQKLANDALVwcplRGLLVGDrnsersgtipgVDMVHAPVALIPMSFPESHWKQACEVAPIFNELVD 152 tomato
544431      6 DDIYN-DTKLLEELAPIAIDAALl---QGVLMRTkesp----nssDVVSFAPFALLPSPVPKALFEQAKCVQEDFNTLVD 77  African clawed frog
P46413      6 GSILQ-DEKQLEELAQQAIDRALa---EGVLLRSaknp----sssDVVTYAPFTLFPSPVPSTLLEQAYAVQMDFNILVD 77  Norway rat
7506074    14 PRLPL-EDDKLNELTADLHDWAHa---NGLVMRLstdk----lssEVCQTTPLTLLPSPFPKNVFEEAVHIQNLFASLYH 85  Caenorhabditis ele...
AAL41009    1 MEIEKyTPEQIEELGKGARDFAFa---HGVVFTElsvs---kegrNIATQIPITLFPSVIPHGAFVEAVSVQKAYNKLYA 74  fission yeast
2495096     2 AHYPP-SKDQLNELIQEVNQWAIt---NGLSMYPpkfe----enpSNASVSPVTIYPTPIPRKCFDEAVQIQPVFNELYA 73  baker's yeast
Feature 1                                                               #                     
2HGS_A     78 AVSQN----AAFLEQTLSSTIKQD-DFTARLFDIHKQVLKE------GIAQTVFLGLNRSDYMFQRSad-gSPALKQIEI 145 human
AAK77663  100 RVSLD----GKFLQESLSRTKEVD-EFTSRLLDIHSKMLAI------NKKEEIRLGLHRSDYMLDEK----TKSLLQIEL 164 Lotus japonicus
AAF98157  150 RVSLD----GKFLQESLSRTKNAD-EFTSRLLDIHSKMLQI------NKKEDIRLAIVRSDYMVDEK----TNSLLQIEM 214 French bean
1170037    85 RVSLD----GKFLQDSLSRTKKVD-VFTSRLLDIHSKMLER------NKKEDIRLGLHRFDYMLDEE----TNSLLQIEM 149 thale cress
T04336    153 RVSQD----GEFLQQSLSRTRKAD-PFTSRLLEIHSKMLEI------NKLEEIRLGLHRSDYMLDEQ----TKLLLQIEL 217 tomato
544431     78 RISQD----TSFLEQVLSSTIKVD-DFIRRLFAIHKQVQQE------DCTQEVFLGINRSDYMFDCRdd-gTPALKQIEI 145 African clawed frog
P46413     78 AVSQN----SAFLEQTLSSTIKKD-EYTARLFDIYKQVLKE------GIAQTVFLGLNRSDYMFQCSad-gSKALKQIEI 145 Norway rat
7506074    86 FIAYE----FDFLIDIHKNVVKTD-DFTRNMVEILKKVKAQ------GLKQPVTLAIQRSDYMCHKDqysaEYGLKQIEI 154 Caenorhabditis ele...
AAL41009   75 KIAND----YEFLRLHLQSITKYD-EFMNKLWNLYQKHREAvahlkeNQFQPLSLGVFRSDYTVHQDd--sFIGCKQVEF 147 fission yeast
2495096    74 RITQDmaqpDSYLHKTTEALALSDsEFTGKLWSLYLATLKSa----qYKKQNFRLGIFRSDYLIDKKk--gTEQIKQVEF 147 baker's yeast
Feature 1          #                                                                          
2HGS_A    146 NTISASFGGLASRTPAVHRHVLSVLs------KTKEAGKILSNNpSKGLALGIAKAWELYGSPn---------------A 204 human
AAK77663  165 NTISSSFAGLGSLLTELHRYILSRYgk----lLGLDSGKVPANNaATQFAEGLARAWTEYNDPr---------------A 225 Lotus japonicus
AAF98157  215 NTISTSFALIGCLMTGLHKSLISQYgn----iLGLDSNRVPANNaVDQSAEALAKGWSEYNDPr---------------A 275 French bean
1170037   150 NTISCSFPGLSRLVSQLHQSLLRSYgd----qIGIDSERVPINTsTIQFADALAKAWLEYSNPr---------------A 210 thale cress
T04336    218 NTISSSFPGLSCLVSELHRSLLQQYre----dIASDPNRIPANNaVNQFAEALAKAWNEYGDPr---------------A 278 tomato
544431    146 NTIAASFGGLASRTPAVHQHVLKFLr------KSEESSSILTNDaVEGIGWGIAHAWALYGSVd---------------A 204 African clawed frog
P46413    146 NTISASFGGLASRTPAVHRHVLNVLn------KTNEASKILSNNpSKGLALGIAKAWELYGSAn---------------A 204 Norway rat
7506074   155 NNIASSMGAHALRLTEWHIRVLKALni----sDDVIQRAIPENKpIPMIAEALFKAWSHFSNPa---------------A 215 Caenorhabditis ele...
AAL41009  148 NTISVSFGGVSKAVSNLHAYCSQSGly----rKPLTTNYLTVNTsVSGICTGISNAVDAYRDYvknitskmniasdntkP 223 fission yeast
2495096   148 NTVSVSFAGLSEKVDRLHSYLNRANkydpkgpIYNDQNMVISDS-GYLLSKALAKAVESYKSQqsss--------ttsdP 218 baker's yeast
Feature 1                                                                                     
2HGS_A    205 LVLLIAQEKERNIFDQRAIENELLARn---IHVIRRTFEDISEKGSLDQ--DRRLFVD----GQEIAVVYFRDGYMPRQY 275 human
AAK77663  226 VIMIVVQAEERNMYDQHFLSAVLRDRh--qIAIVRKTLAEVDREGEILP--DGTLSVD----GQRIAVVYFRAGYTPVDY 297 Lotus japonicus
AAF98157  276 AILVVVQVEERNMYEQHYISALLRDKy--hIKSIRKTLAEVDQEGEILP--DGTLSVD----GQPISVVYFRAGYTPKDY 347 French bean
1170037   211 VVMVIVQPEERNMYDQHLLSSILREKh--nIVVIRKTLAEVEKEGSVQE--DETLIVG----GQAVAVVYFRSGYTPNDH 282 thale cress
T04336    279 VIIFVVQAEERNMYDQHWLSASLRERh--qVTTIRKTLAEIDALGELQQ--DGTLVVD----GQAVAVIYFRAGYAPSDY 350 tomato
544431    205 TVMFLVENEQRNILDQRFIEAELCKRn---VRVIRRRLADVFERGTLDE--ERHLFID----GYEVAVAYFRTGYVPQDY 275 African clawed frog
P46413    205 VVLLIAQEKERNIFDQRAIENELLDRk---IHVIRRRFEDVSERGSLDQ--NRRLFME----DQEVAVVYFRDGYMPSQY 275 Norway rat
7506074   216 VVLVVVENVNQNQIDQRHVEYELEKLgvpmTCIIRRNLTQCYEQLSLND--RSDLMID----GRQVAIVYFRAGYSPDHY 289 Caenorhabditis ele...
AAL41009  224 IVLFVVKGGERNITDQRTLEYELLNR----FHVISKRIDIAELNSLIHDksSNKLYMKtsftTYEVAVVYYRVGYALDDY 299 fission yeast
2495096   219 IVAFIVQRNERNVFDQKVLELNLLEKf--gTKSVRLTFDDVNDKLFIDDk-TGKLFIRd--tEQEIAVVYYRTGYTTTDY 293 baker's yeast
Feature 1                                                                                     
2HGS_A    276 -SLQNWEARLLLERSHAAKCPDIATQLAGTKKVQQELSRPGMLEMLLpgQPEAVARLRATFAGLYSLDvgeeGDQAIAEA 354 human
AAK77663  298 pSESEWRARLLMEQSSAIKCPSISYHLVGTKKIQQELANPGVLERFLe-NKGDIAKLRECFAGLWSLD----DYDITRKA 372 Lotus japonicus
AAF98157  348 pSESEWRAGLLMEQSSAIKCPSISYHLVGTKKIQQELAKPGVLERFVe-NKDHVAKLRACFAGLWSLE----DSDIAQKA 422 French bean
1170037   283 pSESEWNARLLIEESSAVKCPSIAYHLTGSKKIQQELAKPGVLERFLd-NKEDIAKLRKCFAGLWSLD----DSEIVKQA 357 thale cress
T04336    351 hSESEWKARLLMEQSRAVKCPSISYHLAGSKKIQQELAKPNVLERFLe-NKDDIAKLRKCFAGLWSLD----ESDIVKDA 425 tomato
544431    276 -TEQDWEARLMLERSRAVKCPDVPTQLVGTKKVQQELSRPQILEKFLpdKPEAVARIRETFTGLYSLDigeeGDEAVRVA 354 African clawed frog
P46413    276 -NAQNWEARLLLERSCAAKCPDIATQLAGTKKVQQELSRVGLLEALLpgQPEAVARLRATFAGLYSLDmgeeGDQAVAEA 354 Norway rat
7506074   290 pSTKEWEARERMELSTAIKTPWIGLQVANTKKTQQVLSEDGVLERFIg-KPREARDIRASFAGMWALEn---TDEVTMKV 365 Caenorhabditis ele...
AAL41009  300 pSQEAWDMRLTIENTLAIKCPSISTHLAGSKKIQQVLAESNALERFL--EGDELQAVRSTFADMYPLDdtprGKEGIKLA 377 fission yeast
2495096   294 tSEKDWEARLFLEKSFAIKAPDLLTQLSGSKKIQQLLTDEGVLGKYIs-DAEKKSSLLKTFVKIYPLDdtklGREGKRLA 372 baker's yeast
Feature 1                       #                                                             
2HGS_A    355 LA----APSRFVLKPQREGGGNNLYGEEMVQALKQLk--dSEEr---aSYILMEKIEPEPFENCLLRPgSPARVVQCISE 425 human
AAK77663  373 IE----KPELFVMKPQREGGGNNIYGNAVRETLLKLqqagSQEd---aAYILMQRIFPTLSAAVLMRN-GCWHKDHAVSE 444 Lotus japonicus
AAF98157  423 IE----NPELFVMKSQREGGGNNIYGDDMRENLLKLqkagSEEd---aAYILMQRIFPATFPAILVRD-GNWSTGHVIAE 494 French bean
1170037   358 IE----KPGLFVMKPQREGGGNNIYGDDVRENLLRLqkegEEGn---aAYILMQRIFPKVSNMFLVRE-GVYHKHQAISE 429 thale cress
T04336    426 IE----RPELYVMKPQREGGGNNIYGEDVRGALLKLq---KEGtgsdaAYILMQRIFPKISHSILMRE-GISHKEETISE 497 tomato
544431    355 LA----NPDQFVLKPQREGGGNNLYGEELKEKLQECk--dSEEr---tSYILMDKINPKPLKNCLLRAgGRVQISECISE 425 African clawed frog
P46413    355 LA----APSHFVLKPQREGGGNNFYGEEMVHALEQLk--dSEEr---aSYILMEKIEPEPFRNCLLRPgSPAQVVQCISE 425 Norway rat
7506074   366 VAgaqkHPEAFVLKPQTEGGAALHTGDEMVQMLRELp---EEEr---gAFILMEKLKPMIIENYLVLAkKPITFAKAVSE 439 Caenorhabditis ele...
AAL41009  378 FE----KPEDFVLKPQREGGGNNTYGKDIPGLLSKMp---QEEw---dSYILMRYINAVPSQNYILKG-ERPEKFDVVDE 446 fission yeast
2495096   373 LS----EPSKYVLKPQREGGGNNVYKENIPNFLKGI----EERhw--dAYILMELIEPELNENNIILRdNKSYNEPIISE 442 baker's yeast
Feature 1                               #                        
2HGS_A    426 LGIFGVYVRQe--KTLVMNKHVGHLLRTKAIEHADGGVAAGVAVLDNPYPV 474 human
AAK77663  445 LGIFSTYLRNk--DRVVMNNQSGYLMRTKISSSDEGGVAAGFAVLDSVYLT 493 Lotus japonicus
AAF98157  495 AGIFGTYLRNk--DKIIINNESGYMVRTKVSSSYEGGVLPGFGVVDTVYLT 543 French bean
1170037   430 LGVYGAYLRSk--DEVIVNEQSGYLMRTKIASSDEGGVAAGFGVLDSIYLI 478 thale cress
T04336    498 LGIYGTYLRNk--TEVLINQQAGYLMRTKVSSSDEGGVAAGFAVLDSIYLV 546 tomato
544431    426 LGMFGVYVRHr--DQMIYYDQVGHLLRTKAIEHSDGGVAAGVAVLDNPYLV 474 African clawed frog
P46413    426 LGIFGVYVRQg--TTLVMNKHVGHLLRTKAIEHADGGVAAGVAVLDNPYPV 474 Norway rat
7506074   440 LGVYGYAFGR---KDAPELKTAGHLLRTKPESTAMGGVAAGHAVVDTPFLY 487 Caenorhabditis elegans
AAL41009  447 IGILGTIVWNiktDEVVQNGQSGFICRTKPKKTNEGGVATGYASLSSIELS 497 fission yeast
2495096   443 LGIYGCVLFNd--EQVLSNEFSGSLLRSKFNTSNEGGVAAGFGCLDSIILY 491 baker's yeast

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap