Table S2. An updated list of analogous enzymes.

This listing was originally prepared as a web supplement to the article by M.Y. Galperin, D.R. Walker and E.V. Koonin
Analogous enzymes: Independent inventions in enzyme evolution, published in 1998 in Genome Research 8: 779-790.
Abstract. Full text (HTML). An updated list of the 105 EC nodes described in that paper is available here.

This version is a supplementary table to the paper by M.V. Omelchenko, M.Y. Galperin, Y.I. Wolf and E.V. Koonin
Non-homologous isofunctional enzymes: A systematic analysis of alternative solutions in enzyme evolution,
published in Biology Direct 2010, 5:31. Abstract. Full text (HTML).

Submission of additional examples of analogous (non-homologous isofunctional) enzymes is most welcome.
Please send the respective PubMed IDs to Michael Galperin

No. EC no. Enzyme name NCBI UniProt PDB SCOP fold Super-family Family (Pfam) Ref. Comments
1 1.1.1.1 Alcohol dehydrogenase P10127 ADH4_YEAST 2BI4 Dehydroquinate synthase-like SupFam Iron-contaning alcohol dehydrogenase 3282541, 2823079 Two distinct structural folds
1.1.1.1 Alcohol dehydrogenase P00334 ADH_DROME 1MG5 Rossmann-fold SupFam Short chain dehydrogenase 15016358, 11669613
2 1.1.1.2 Alcohol dehydrogenase (NADP+) P0A4X1 ADHC_MYCBO 1YQD GroES-like and Rossmann-fold SupFam Zinc-contaning alcohol dehydrogenase 1427101, 15829607 Three distinct structural folds
1.1.1.2 Alcohol dehydrogenase (NADP+) P14550 AK1A1_HUMAN 2ALR TIM beta/alpha-barrel SupFam Aldo/keto reductase 15299353, 10486210
1.1.1.2 Alcohol dehydrogenase (NADP+) Q09669 ADH4_SCHPO 1O2D Dehydroquinate synthase-like SupFam Iron-contaning alcohol dehydrogenase 15327949, 14705036
3 1.1.1.27 L-lactate dehydrogenase P00338 LDHA_HUMAN 1EZ4 Rossmann-fold and LDH C-terminal domain-like SupFam Lactate AND malate dehydrogenases 11807949 Two distinct structural folds
1.1.1.27 L-lactate dehydrogenase Q07251 LDH_RALEH 1VBI L-sulfolactate dehydrogenase-like SupFam Malate/L-Lactate dehydrogenases 8405966
4 1.1.1.28 D-lactate dehydrogenase P52643 LDHD_ECOLI 2DLD Rossmann-fold and Flavodoxin-like SupFam Formate/glycerate dehydrogenases 9025293 Two distinct structural folds
1.1.1.28 D-lactate dehydrogenase P06149 DLD_ECOLI 1F0X FAD-binding and Ferredoxin-like SupFam D-lactate dehydrogenase 10944213
5 1.1.1.37 Malate dehydrogenase Q07841 MDH_HALMA 2J5K Rossmann-fold and LDH C-terminal domain-like SupFam L-lactate/malate dehydrogenases 8476859, 10653643 Two distinct structural folds
1.1.1.37 Malate dehydrogenase P16142 MDH_METFE 2G8Y L-sulfolactate dehydrogenase-like SupFam Malate/L-Lactate dehydrogenases 2110059, 10850983
6 1.1.1.50 3-alpha-hydroxysteroid dehydrogenase (B-specific) Q9ZFY9 Q9ZFY9_COMTE
(DIDH_COMTE)		 1FJH Rossmann-fold SupFam Short chain dehydrogenase 8944761, 10833462, 11007791 Two distinct structural folds
1.1.1.50 3-alpha-hydroxysteroid dehydrogenase (B-specific) P17516 AK1C4_HUMAN 2FVL TIM beta/alpha-barrel SupFam Aldo/keto reductase 1530633, 11158055
7 1.1.1.62 Estradiol 17-ß-dehydrogenase P14061 DHB1_HUMAN 1FDS Rossmann-fold SupFam Short chain dehydrogenase 2846351, 7663947 Two distinct structural folds
1.1.1.62 Estradiol 17-ß-dehydrogenase P70694 DHB5_MOUSE 1Q5M TIM beta/alpha-barrel SupFam Aldo/keto reductase 7737980
8 1.1.1.63 Testosterone 17-ß-dehydrogenase O14756 H17B6_HUMAN similar to 1I01 Rossmann-fold SupFam Short-chain dehydrogenase 11513953, 11360992 Two distinct structural folds
1.1.1.63 Testosterone 17-ß-dehydrogenase P42330 AK1C3_HUMAN 1S1P TIM beta/alpha-barrel SupFam Aldo/keto reductase 14996743, 9927279, 15087468
9 1.1.1.64 Testosterone 17-ß-dehydrogenase (NADP+) P37058 DHB3_HUMAN 2C07 Rossmann-fold SupFam Short-chain dehydrogenases 2558148 Two distinct structural folds
1.1.1.64 Testosterone 17-ß-dehydrogenase (NADP+) P42330 AK1C3_HUMAN 1S1P TIM beta/alpha-barrel SupFam Aldo/keto reductase 14996743, 9927279
10 1.1.1.82 Malate dehydrogenase (NADP+) Q60176 MDH_METJA 1HYG Rossmann-fold and LDH C-terminal domain-like SupFam Lactate/malate dehydrogenase 10850983, 11292347 Two distinct structural folds
1.1.1.82 Malate dehydrogenase (NADP+) P16142 MDH_METFE 1RFM L-sulfolactate dehydrogenase-like SupFam Malate/L-lactate dehydrogenase 2110059
11 1.1.1.189 Prostaglandin-E2 9-reductase P16152 CBR1_HUMAN 1WMA Rossmann-fold SupFam Short-chain dehydrogenase 8421682 Two distinct structural folds
1.1.1.189 Prostaglandin-E2 9-reductase P80508 PE2R_RABIT 1Q13 TIM beta/alpha-barrel SupFam Aldo/keto reductase 8529651
12 1.1.1.283 Methylglyoxal reductase O32210 GR_BACSU 1MZR TIM beta/alpha-barrel SupFam Aldo/keto reductase 16232966 Two distinct structural folds
1.1.1.283 Methylglyoxal reductase Q12068 GRE2_YEAST 1Y1P Rossmann-fold SupFam NAD dependent epimerase/dehydratase 3896793, 12722185
13 1.2.1.2 Formate dehydrogenase P07658 FDHF_ECOLI 1FDI Formate dehydrogenase/DMSO reductase SupFam Molybdopterin-containing oxidoreductases 9036855 Two distinct structural folds
1.2.1.2 Formate dehydrogenase P33160 FDH_PSESR 2NAC Rossmann-fold and Flavodoxin-like SupFam 2-hydroxyacid dehydrogenase 1597184, 8484798
14 1.2.1.10 Acetaldehyde dehydrogenase (acetylating) P77580 MHPF_ECOLI, MHPF_PSEPU 1NVM Rossmann-fold and FwdE/ GAPDH domain-like SupFam Prokaryotic acetaldehyde dehydrogenase 16782065, 11264589, 12764229 Two distinct structural folds
1.2.1.10 Acetaldehyde dehydrogenase (acetylating) Q24803 ADH2_ENTHI N-term is similar to 1AD3 Predicted: ALDH-like SupFam Aldehyde dehydrogenase 7980441
15 1.2.99.2 Carbon-monoxide dehydrogenase (acceptor) P19919 DCML_OLICO 1N60 CO dehydrogenase ISP C-domain like and others SupFam Aldehyde oxidase and xanthine dehydrogenase 10482497, 12475995 Two distinct structural folds
1.2.99.2 Carbon-monoxide dehydrogenase (acceptor) P31896 COOS_RHORU 1JQK Prismane protein-like SupFam Prismane/CO dehydrogenase 8561463, 11593006
16 1.3.1.34 2,4-dienoyl-CoA reductase (NADPH) P42593 FADH_ECOLI 1PS9 TIM beta/alpha-barrel SupFam Oxidoreductase / NADH oxidase family 12840019 Two distinct structural folds
1.3.1.34 2,4-dienoyl-CoA reductase (NADPH) Q9NUI1 DECR2_HUMAN 1W6U Rossmann-fold SupFam Short-chain dehydrogenases 15531764
17 1.5.1.29 FMN reductase P0AEN1 FRE_ECOLI 1QFJ Reductase/isomerase/elongation factor common domain and Ferredoxin reductase-like C-terminal NADP-linked domain SupFam NAD(P)H-flavin reductase 2050627, 10353815 Two distinct structural folds
1.5.1.29 FMN reductase Q07923 LOT6_YEAST 1T0I Flavodoxin-like SupFam FMN reductase 15184374
18 1.5.1.34 6,7-dihydropteridine reductase P09417 DHPR_HUMAN 1HDR Rossmann-fold SupFam Short-chain dehydrogenase 3033643 Two distinct structural folds
1.5.1.34 6,7-dihydropteridine reductase P38489 NFNB_ECOLI 1ICR FMN-dependent nitroreductase-like SupFam Nitroreductase 3060113, 11491290, 15684426
19 1.6.99.3 NADH dehydrogenase P00393 DHNA_ECOLI 1XHC FAD/NAD(P)-binding domain SupFam NADH dehydrogenase 1917890 Two distinct structural folds
1.6.99.3 NADH dehydrogenase P28331 NDUS1_HUMAN 2FUG Formate dehydrogenase/DMSO reductase SupFam Complex I 75 kDa subunit family 1935949
20 1.7.2.1 Nitrite reductase (NO-forming) P38501 NIR_ALCFA 2AFN Cupredoxin-like SupFam Multicopper oxidase 8515232 Two distinct structural folds
1.7.2.1 Nitrite reductase (NO-forming) P24474 NIRS_PSEAE 1NIR Cytochrome c and 8-bladed beta-propeller SupFam Cytochrome D1 heme domain (Complex I 75 kDa subunit family) 2506077, 11226222
21 1.11.1.5 Cytochrome-c peroxidase P14532 CCPR_PSEAE 1EB7 Cytochrome c SupFam Di-heme cytochrome c peroxidase 7781769, 1657179, 8591033 Two distinct structural folds
1.11.1.5 Cytochrome-c peroxidase P00431 CCPR_YEAST 1EBE Heme-dependent peroxidases SupFam Peroxidase 2169873, 6092361
22 1.11.1.6 Catalase P04040 CATA_HUMAN 1QQW Heme-dependent catalase-like SupFam Heme-dependent catalase 10666617, 3755525 Three distinct structural folds
1.11.1.6 Catalase P13029 CATA_ECOLI 2FXG Heme-dependent peroxidases SupFam Peroxidase 374409, 2670897, 8508796
1.11.1.6 Catalase P60355 MCAT_LACPL 1JKU Ferritin-like SupFam Manganese catalase 8939876
23 1.11.1.7 Peroxidase O08709 PRDX6_MOUSE 1PRX Thioredoxin fold SupFam Thioredoxin 9291135, 10395907, 9587003 At least two distinct structural folds
1.11.1.7 Peroxidase P05164 PERM_HUMAN 1MYP Heme-dependent peroxidases SupFam Animal heme peroxidase 2154223
1.11.1.7 Peroxidase P31545 YCDB_ECOLI similar to 2d3q Predicted: Ferredoxin-like SupFam Dyp-type peroxidase-like 16551627, 14684913
24 1.11.1.10 Chloride peroxidase P25026 PRXC_PSEPY 1A88 Alpha/beta hydrolase SupFam Haloperoxidase 8344520 Two distinct structural folds
1.11.1.10 Chloride peroxidase P49053 PRXC_CURIN 1VNC Acid phosphatase/Vanadium-dependent haloperoxidase SupFam Type 2 phosphatidic acid phosphatase (PAP2) 9165086
1.11.1.10 Chloride peroxidase P04963 PRXC_CALFU 2CPO EF Hand-like SupFam Cloroperoxidase 8747463, 11278701
25 1.11.1.15 Peroxiredoxin Q9Y9L0 TDXH_AERPE 2E2G Thioredoxin fold SupFam AhpC/TSA family 18436649 Two distinct structural folds
1.11.1.15 Peroxiredoxin P0A5N4 AHPD_MYCTU 1KNC AhpD-like SupFam Carboxymuconolactone decarboxylase 11914371, 10766746, 11799204, 12761216
1.11.1.15 Peroxiredoxin P0C0L2 OSMC_ECOLI 1NYE OsmC-like SupFam OsmC-like 14627744, 15103136
26 1.12.2.1 Cytochrome-c3 hydrogenase (was 1.18.99.1) P12944 PHNL_DESGI 1FRV HydA/Nqo6-like and HydB/Nqo4-like SupFam Ni-Fe hydrogenase 2651421, 7854413 Two distinct structural folds
1.12.2.1 Cytochrome-c3 hydrogenase (was 1.18.99.1) P29166 PHF1_CLOPA 1FEH Fe-only hydrogenase SupFam Iron-only hydrogenase 1911757, 9836629
27 1.14.99.3 Heme oxygenase P09601 HMOX1_HUMAN 1N3U Heme oxygenase-like SupFam Heme oxygenase 12500973, 15285018, 15049686, 15522396 Two distinct structural folds
1.14.99.3 Heme oxygenase Q6GKE0 ISDI_STAAR 1XBW Ferredoxin-like SupFam Antibiotic biosynthesis monooxygenase 14570922, 15774886
28 1.15.1.1 Superoxide dismutase P09157 SODF_ECOLI 1ISA FeMn superoxide dismutase SupFam Fe,Mn superoxide dismutase 2447093 Two distinct structural folds
1.15.1.1 Superoxide dismutase P53635 SODC_ECOLI 1ESO Immunoglobulin-like beta-sandwich SupFam Cu,Zn superoxide dismutase 9405149
1.15.1.1 Superoxide dismutase P80734 SODN_STRSO 1Q0D Four-helical up-and-down bundle SupFam Nickel superoxide dismutase 15173586
29 1.16.3.1 Ferroxidase P02794 FRIH_HUMAN, FTNA_ECOLI 2CEI, 1BCF Ferritin-like SupFam Ferritin, Ferritin-like domain 7982945, 15201052 Two distinct structural folds
1.16.3.1 Ferroxidase P00450 CERU_HUMAN 1KCW Cupredoxin-like SupFam Multicopper oxidase 17242517, 12055353
30 1.18.1.2 Ferredoxin-NADP+ reductase Q44532 FENR_AZOVI 1A8P Reductase/isomerase/elongation factor common domain and Ferredoxin reductase-like C-terminal NADP-linked domain SupFam Oxidoreductase FAD-binding and NAD-binding domains 8034707, 9865948, 9149148, 8449868 Two distinct structural folds
1.18.1.2 Ferredoxin-NADP+ reductase O05783 FPRA_MYCTU 1LQT FAD/NADP-binding domain and Nucleotide-binding domain SupFam Pyridine nucleotide-disulphide oxidoreductase 12071965
31 1.20.4.1 Arsenate reductase (glutaredoxin) P08692 ARSC1_ECOLX 1I9D Thioredoxin fold SupFam ArsC family 11709171 Two distinct structural folds
1.20.4.1 Arsenate reductase (glutaredoxin) Q06597 ACR2_YEAST 1C25 Rhodanese/Cell cycle control phosphatase SupFam Rhodanese 10801893
32 2.1.1.43 Histone-lysine N-methyltransferase Q8WTS6 SETD7_HUMAN 1N6C Beta-clip (catalytic domain) SupFam Histone-lysine methyltransferase 12540855, 12372304 Two distinct structural folds
2.1.1.43 Histone-lysine N-methyltransferase Q04089 DOT1_YEAST 1U2Z S-adenosyl-L-methionine-dependent methyltransferases SupFam Histone methylation protein 15292170, 12086673, 12080090
33 2.3.1.28 Chloramphenicol O-acetyltransferase P23364 CAT4_AGRTU 1XAT Single-stranded left-handed beta-helix SupFam Galactoside acetyltransferase 2013403, 9578552 Two distinct structural folds
2.3.1.28 Chloramphenicol O-acetyltransferase P00484 CAT3_ECOLX 3CLA CoA-dependent acyltransferases SupFam Chloramphenicol acetyltransferase 2187098
34 2.3.2.5 Glutaminyl-peptide cyclotransferase Q16769 QPCT_HUMAN 2AFM Phosphorylase/hydrolase-like SupFam Peptidase family M28 16135565, 15063747 Two distinct structural folds
2.3.2.5 Glutaminyl-peptide cyclotransferase O81226 O81226_CARPA 2FAW, similar to 1VKD 5-bladed beta-propeller ( paper) SupFam Glutamine cyclotransferase 11035947, 17081122
35 2.4.1.25 4-alpha-glucanotransferase P15977 MALQ_ECOLI similar to 1FP8 TIM beta/alpha-barrel SupFam Glycoside hydrolase family 77 2845225 Two distinct structural folds
2.4.1.25 4-alpha-glucanotransferase O32462 MALQ_THELI 1K1X 7-stranded beta/alpha barrel SupFam Glycosyl hydrolase family 57 12618437
36 2.5.1.3 Thiamine-phosphate synthase P30137 THIE_ECOLI 2TPS TIM beta/alpha-barrel SupFam Thiamine monophosphate synthase 10350464 Two distinct structural folds (no EC)
2.5.1.3 Thiamine-phosphate synthase O26949 O26949_METTH 2PHP AraD/HMP-PK domain-like SupFam Archaeal phosphomethylpyrimidine kinase 12794638
37 2.5.1.17 Cob(I)yrinic acid a,c-diamide adenosyltransferase P31570 BTUR_SALTY 1G5T P-loop containing NTPases SupFam ATP:corrinoid adenosyltransferase BtuR/CobO/CobP 7860601 Two distinct structural folds
2.5.1.17 Cob(I)yrinic acid a,c-diamide adenosyltransferase Q96EY8 MMAB_HUMAN 2IDX Ferritin-like SupFam Cobalamin adenosyltransferase 12514191, 17176040
38 2.5.1.18 Glutathione transferase P39100 GST_ECOLI, GSTK1_HUMAN 1A0F, 1R4W Thioredoxin fold and GST C-terminal domain-like SupFam Glutathione S-transferase, class beta, class kappa 2185038, 16081649, 14717589 Three distinct structural folds
2.5.1.18 Glutathione transferase P10620 MGST1_HUMAN 2H8A MAPEG domain-like SupFam MAPEG family 8812420, 9278457, 10091672, 16806268
2.5.1.18 Glutathione transferase Q56415 FOSA_SERMA 1NPB Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase SupFam Antibiotic resistance proteins 15075406, 9115979
39 2.5.1.47 Cysteine synthase P47998 CYSK1_ARATH 1Z7W Tryptophan synthase beta subunit-like PLP-dependent enzymes SupFam Pyridoxal-phosphate dependent enzyme 16166087 Two distinct structural folds
2.5.1.47 Cysteine synthase P06106 MET17_YEAST 1GC0 PLP-dependent transferase-like SupFam Cys/Met metabolism PLP-dependent enzyme 7765825, 8511969
40 2.7.1.4 Fructokinase P40713 SCRK_ECOLI similar to 1TZ6 Ribokinase-like SupFam PfkB family carbohydrate kinase 8278523 Two distinct structural folds
2.7.1.4 Fructokinase Q07211 SCRK_STRMU 1XC3 Ribonuclease H-like motif SupFam Actin-like ATPase domain 8336109
41 2.7.1.11 6-phosphofructokinase P06998 K6P1_ECOLI 1PFK Phosphofructokinase SupFam Phosphofructokinase 2975709 Two distinct structural folds
2.7.1.11 6-phosphofructokinase P06999 K6PF2_ECOLI 3CQD Ribokinase-like SupFam PfkB family carbohydrate kinase 18762190, 6310120
42 2.7.1.12 Gluconokinase P46859 GNTK_ECOLI 1KNQ P-loop containing NTPases SupFam Gluconate kinase 8655507, 11468405 Two distinct structural folds
2.7.1.12 Gluconokinase P12011 GNTK_BACSU 3GBT, 2ITM Ribonuclease H-like motif SupFam Glycerol kinase 3020045
43 2.7.1.31 Glycerate kinase P23524 GLXK2_ECOLI 1TO6 Glycerate kinase I SupFam Glycerate kinase type-1 5325263, 166273 Two distinct structural folds
2.7.1.31 Glycerate kinase Q944I4 GLYK_ARATH similar to 1ODF P-loop containing NTPases SupFam GLYK kinase family (UniProt) 15980259
2.7.1.31 Glycerate kinase Q8IVS8 GLCTK_HUMAN smilar to 2B8N GckA/TtuD-like SupFam Glycerate kinase type-2 (contains MOFRL domain) 16753811, 16865707 Produces 2-phospho-glycerate
44 2.7.1.33 Pantothenate kinase O25533 COAX_HELPY 2NRH Ribonuclease H-like motif SupFam Type III pantothenate kinase 15795230, 16905099 Two distinct structural folds
2.7.1.33 Pantothenate kinase P0A6I3 COAA_ECOLI 1SQ5 P-loop containing NTPases SupFam Phosphoribulokinase/Uridine kinase family 15136582, 1328157
45 2.7.1.39 Homoserine kinase P17423 KHSE_YEAST 1FWK Ribosomal protein S5 domain 2-like and Ferredoxin-like SupFam GHMP kinase 2165904 Two distinct structural folds
2.7.1.39 Homoserine kinase P29364 KHSE_PSEAE 2PPQ Protein kinase-like (PK-like) SupFam Phosphotransferase 1333566
46 2.7.1.71 Shikimate kinase P0A6E1 AROL_ECOLI 1SHK P-loop containing NTPases SupFam Shikimate kinase 3001029, 9600856, 11369852 Two distinct structural folds
2.7.1.71 Shikimate kinase Q58835 AROK_METJA similar to 1VIS Ribosomal protein S5 domain 2-like and Ferredoxin-like SupFam GHMP kinase 11114929
47 2.7.1.107 Diacylglycerol kinase P49619 DGKG_HUMAN 2QV7 NAD kinase/diacylglycerol kinase-like SupFam Diacylglycerol kinase catalytic domain 18611377 Two distinct structural folds
2.7.1.107 Diacylglycerol kinase P0ABN1 KDGL_ECOLI - Integral membrane protein SupFam Prokaryotic diacylglycerol kinase 2984194, 8071224
48 2.7.4.1 Polyphosphate kinase P0A7B1 PPK_ECOLI 1XDO Spectrin repeat-like and Phospholipase D/nuclease and PHP14-like SupFam Polyphosphate kinase 8380170, 1331061 Two distinct structural folds
2.7.4.1 Polyphosphate kinase Q8GCQ3 Q8GCQ3_PSEAE (PPK2) 3CZQ 3-layer Alpha/beta/alpha sandwich fold (paper), contains P-loop SupFam Polyphosphate kinase 2 12486232, 19001261
49 2.7.4.2 Phosphomevalonate kinase P24521 ERG8_YEAST similar to 1FWL Ribosomal protein S5 domain 2-like and Ferredoxin-like SupFam GHMP_kinases 1846667 Two distinct structural folds
2.7.4.2 Phosphomevalonate kinase Q15126 PMVK_HUMAN 3CH4 P-loop containing NTPases SupFam Phosphomevalonate kinase 10191291, 16519518, 17902708
50 2.7.10.2 Non-specific protein-tyrosine kinase P06241 FYN_HUMAN 2H8H Protein kinase-like (PK-like) SupFam Protein tyrosine kinase 15557120 At least two distinct structural folds
2.7.10.2 Non-specific protein-tyrosine kinase Q54520 CPSD2_STRPN similar to 1G3Q P-loop containing NTPases SupFam CbiA 10760144
2.7.10.2 Non-specific protein-tyrosine kinase Q9UIG0 BAZ1B_HUMAN - - SupFam Pfam hit 19092802
51 3.1.1.2 Arylesterase Q07792 ESTE_VIBMI similar to 1IVN Flavodoxin-like SupFam GDSL-like Lipase/Acylhydrolase 8141782 Two distinct structural folds
3.1.1.2 Arylesterase P22862 ESTE_PSEFL 1VA4 Alpha/beta hydrolase SupFam Alpha/beta hydrolase 15213385, 7704276, 1368608
3.1.1.2 Arylesterase P27169 PON1_HUMAN 1V04 6-bladed beta-propeller SupFam Arylesterase 8393742, 1718413, 1673382, 15098021
52 3.1.1.4 Phospholipase A2 Q9NZK7 PA2GE_HUMAN 1POE Phospholipase A2 PLA2 SupFam Phospholipase A2 10681567 Two distinct structural folds
3.1.1.4 Phospholipase A2 P0A921 PA1_ECOLI 1QD5 Transmembrane beta-barrels SupFam Phospholipase A1 8300539, 10537112
3.1.1.4 Phospholipase A2 P47712 PA24A_HUMAN 1CJY FabD/lysophospholipase-like (catalytic domain) SupFam Lysophospholipase catalytic domain 10319815, 8083230, 11416127
53 3.1.1.5 Lysophospholipase O88202 LPP60_RAT N-term is similar to 2OCD Glutaminase/Asparaginase SupFam Asparaginase (N-term) 9575212 At least two distinct structural folds
3.1.1.5 Lysophospholipase P47712 PA24A_HUMAN 1CJY FabD/lysophospholipase-like (catalytic domain) SupFam Lysophospholipase catalytic domain 10319815, 8083230, 11416127
3.1.1.5 Lysophospholipase P07000 PLDB_ECOLI similar to 1A8Q Predicted: Alpha/beta hydrolase SupFam Alpha/beta hydrolase 3908447, 3908445
54 3.1.1.29 Aminoacyl-tRNA hydrolase P0A7D1 PTH_ECOLI 2PTH Phosphorylase/hydrolase-like SupFam Peptidyl-tRNA hydrolase 9303320 Two distinct structural folds
3.1.1.29 Aminoacyl-tRNA hydrolase Q980V1 PTH_SULSO, PTH2_HUMAN 1XTY, 1Q7S Peptidyl-tRNA hydrolase II SupFam Peptidyl-tRNA hydrolase PTH2 12799450, 15766258, 14660562
55 3.1.1.31 6-phosphogluconolactonase Q9EV79 6PGL_PSEPU, 6PGL_THEMA similar to 1VL1 NagB/RpiA/CoA transferase-like SupFam Glucosamine/galactosamine-6-phosphate isomerase (uniprot) 10869070, 11277923, 16359314 Two distinct structural folds
3.1.1.31 6-phosphogluconolactonase P52697 6PGL_ECOLI 1RI6 7-bladed beta-propeller SupFam Cycloisomerase 2 (uniprot) 15576773, 16359314
56 3.1.1.61 Protein-glutamate methylesterase P07330 CHEB_ECOLI 1CHD Methylesterase CheB, C-terminal domain SupFam CheB methylesterase 7608974 Two distinct structural folds
3.1.1.61 Protein-glutamate methylesterase Q9X005 CHED_THEMA 2F9Z CheC-like and CNF1/YfiH-like putative cysteine hydrolases SupFam CheD 16469702
57 3.1.2.1 Acetyl-CoA hydrolase Q9UUJ9 ACH1_SCHPO 2G39 NagB/RpiA/CoA transferase-like SupFam Acetyl-CoA hydrolase/transferase 12606555, 1970569 Two distinct structural folds
3.1.2.1 Acetyl-CoA hydrolase Q99NB7 ACO12_RAT 3B7K (N-term) 3FO5 (C-term) Thioesterase/thiol ester dehydrase-isomerase SupFam Thioesterase superfamily 11322891
58 3.1.3.2 Acid phosphatase Q9NPH0 PPA6_HUMAN similar to 1ND5 Phosphoglycerate mutase-like SupFam Histidine acid phosphatase 12010880, 10506173 At least five distinct structural folds
3.1.3.2 Acid phosphatase P0AE22 APHA_ECOLI 1N8N HAD-like SupFam Class B acid phosphatases 9011040
3.1.3.2 Acid phosphatase P14924 PPA_ZYMMO similar to 1D2T Acid phosphatase/Vanadium-dependent haloperoxidase SupFam Type 2 phosphatidic acid phosphatase (PAP2) 2914872
3.1.3.2 Acid phosphatase P13686 PPA5_HUMAN 1UTE Metallo-dependent phosphatases SupFam Calcineurin-like phosphoesterase 2775236
3.1.3.2 Acid phosphatase P24666 PPAC_HUMAN 5PNT Phosphotyrosine protein phosphatases I-like SupFam Low molecular weight phosphotyrosine protein phosphatase 9705307
3.1.3.2 Acid phosphatase P37274 PHOA_PENCH - - SupFam Phosphoesterase 1563629
59 3.1.3.5 5'-nucleotidase P0A840 SURE_ECOLI 1L5X, 2V4O SurE-like SupFam SurE nucleotidase 19021761, 15489502 Two distinct structural folds
3.1.3.5 5'-nucleotidase B1X903 YFBR_ECODH 2PAQ HD-domain/PDEase-like SupFam Metal dependent phosphohydrolase 15489502
3.1.3.5 5'-nucleotidase O46411 5NTC_BOVIN 2J2C, similar to 2BDE HAD-like SupFam 5' nucleotidase 9371705
60 3.1.3.8 3-phytase P42094 PHYT_BACSU 1POO 6-bladed beta-propeller SupFam Phytase 11104666 Two distinct structural folds
3.1.3.8 3-phytase O00092 PHYA_ASPFU 1QWO Phosphoglycerate mutase-like SupFam Histidine acid phosphatase 9143104, 15136045
61 3.1.3.11 Fructose-bisphosphatase P19912 F16P2_RALEH similar to 1D9Q Carbohydrate phosphatase SupFam FBPase 7767230 At least three distinct structural folds
3.1.3.11 Fructose-bisphosphatase Q45597 F16P_BACSU - Metallo-dependent phosphatases SupFam Firmicute fructose-1,6-bisphosphatase (FBPase 2) 221467, 9696785
3.1.3.11 Fructose-bisphosphatase Q8U359 Q8U359_PYRFU (PF0613) 1UMG Sulfolobus fructose-16-bisphosphatase-like SupFam FBPase 3 12029059, 15274916, 12065581, 15317785
3.1.3.11 Fructose-bisphosphatase P36136 YK23_YEAST 3LL4 Phosphoglycerate mutase-like SupFam Phosphoglycerate mutase 20427268
62 3.1.3.15 Histidinol-phosphatase P06987 HIS7_ECOLI 2FPR HAD-like SupFam Polynucleotide kinase 3 phosphatase 16966333 Two distinct structural folds
3.1.3.15 Histidinol-phosphatase O34411 HIS9_BACSU 3DCP 7-stranded beta/alpha barrel SupFam Polymerase/Histidinol Phosphatase 10322033, 17929834
63 3.1.3.16 Phosphoprotein phosphatase P55798 PRP1_ECOLI 1G5B Metallo-dependent phosphatases SupFam Calcineurin-like phosphoesterase 9130712 Four distinct structural folds
3.1.3.16 Phosphoprotein phosphatase O34779 PRPC_BACSU 2PK0, similar to C-term of 1TXO PP2C-like SupFam Protein phosphatase 2C 10986276
3.1.3.16 Phosphoprotein phosphatase Q9GZU7 CTDS1_HUMAN 1TA0 HAD-like SupFam NLI interacting factor-like phosphatase 15304220
3.1.3.16 Phosphoprotein phosphatase Q8WTR2 DUS19_HUMAN similar to 1M3G Phosphotyrosine protein phosphatases II SupFam Dual specificity phosphatase (catalytic domain) 12479873
64 3.1.3.33 Polynucleotide 5'-phosphatase O13297 CET1_YEAST 1D8H CYTH-like phosphatases SupFam mRNA capping enzyme, beta chain 9345280, 10589681 At least two distinct structural folds
3.1.3.33 Polynucleotide 5'-phosphatase O60942 MCE1_HUMAN N-term is 1I9S and C-term is similar to 1P16 Phosphotyrosine protein phosphatases II SupFam Dual specificity phosphatase, catalytic domain 9473487, 9371772, 9512541
3.1.3.33 Polynucleotide 5'-phosphatase P04298 MCEL_VACCW - - SupFam Viral GTase family (UniProt) 8662636, 8662635
3.1.3.33 Polynucleotide 5'-phosphatase P08411 POLN_SFV - - SupFam Pfam hits 10748213
65 3.1.3.48 Protein-tyrosine-phosphatase P53433 PTPA_STRCO 1D1P Phosphotyrosine protein phosphatases I-like SupFam Low-molecular-weight phosphotyrosine protein phosphatases 8550407, 1304913 At least three distinct structural folds
3.1.3.48 Protein-tyrosine-phosphatase P23468 PTPRD_HUMAN 1LAR (C-term) Phosphotyrosine protein phosphatases II SupFam Higher-molecular-weight phosphotyrosine protein phosphatases 2170109, 8833149
3.1.3.48 Protein-tyrosine-phosphatase P30307 MPIP3_HUMAN 1QB0 (C-term) Rhodanese/Cell cycle control phosphatase SupFam Cell cycle control phosphatase, catalytic domain 8276463
3.1.3.48 Protein-tyrosine-phosphatase P96717 YWQE_BACSU similar to 2anu Predicted: 7-stranded beta/alpha barrel SupFam PHP domain 15866923
3.1.3.48 Protein-tyrosine-phosphatase P97480 EYA3_MOUSE similar to 1jud Predicted: HAD-like SupFam HAD-like hydrolase 14628042, 14628052
66 3.1.4.52 Cyclic-diguanylate-specific phosphodiesterase P21514 YAHA_ECOLI 2BAS TIM beta/alpha-barrel SupFam Pfam hits 15994307, 15995192 Two distinct structural folds (EC 3.1.4.-)
3.1.4.52 Cyclic-diguanylate-specific phosphodiesterase Q4UU85 RPFG_XANC8 similar to 1F0J HD-domain/PDEase-like SupFam HD domain 16611728
67 3.1.6.1 Arylsulfatase P51691 ARS_PSEAE 1HDH Alkaline phosphatase-like SupFam Sulfatase 7744061, 11435113 Two distinct structural folds
3.1.6.1 Arylsulfatase P28607 ARS_ALTCA similar to 2CBN Metallo-hydrolase/oxidoreductase SupFam Metallo-beta-lactamase 8535517
68 3.1.8.1 Aryldialkylphosphatase P0A434 OPD_BREDI 1PSC TIM beta/alpha-barrel SupFam Phosphotriesterase 7794910 Two distinct structural folds
3.1.8.1 Aryldialkylphosphatase Q15165 PON2_HUMAN 1V04 6-bladed beta-propeller SupFam Arylesterase 8661009, 11579088, 15098021
69 3.1.8.2 Diisopropyl-fluorophosphatase Q7SIG4 DFPA_LOLVU 2GVV 6-bladed beta-propeller SupFam SMP-30/Gluconolaconase/LRE-like 11295437, 11171055 Two distinct structural folds
3.1.8.2 Diisopropyl-fluorophosphatase P77814 PEPQ_PSEHA similar to 2BWX Creatinase/aminopeptidase SupFam Metallopeptidase family M24 9079288
70 3.1.11.2 Exodeoxyribonuclease III P09030 EX3_ECOLI 1AKO DNase I-like SupFam Endonuclease/Exonuclease/phosphatase 7885481, 8948651 Two distinct structural folds
3.1.11.2 Exodeoxyribonuclease III Q9BQ50 TREX2_HUMAN 1Y97 Ribonuclease H-like motif SupFam Exonuclease 15661738, 10391904
3.1.11.2 Exodeoxyribonuclease III O60671 RAD1_HUMAN 3A1J DNA clamp SupFam Repair protein Rad1/Rec1/Rad17 9660799, 9716408
71 3.1.11.3 Exodeoxyribonuclease (lambda-induced) P06229 EXO5_BPT5 1UT5 PIN_domain-like (catalytic domain) SupFam 5'3'-exonuclease, N-terminal resolvase-like domain 3002857, 9874768 Two distinct structural folds
3.1.11.3 Exodeoxyribonuclease (lambda-induced) P03697 EXO_LAMBD 1AVQ Restriction endonuclease-like SupFam YqaJ viral recombinase family 9295273
72 3.1.13.1 Exoribonuclease II P30850 RNB_ECOLI 2IX0 OB-fold SupFam Ribonuclease II (RNB) 8497196 Two distinct structural folds
3.1.13.1 Exoribonuclease II Q96AZ6 ISG20_HUMAN 1WLJ Ribonuclease H-like motif SupFam Exonuclease 11401564, 12594219
73 3.1.21.1 Deoxyribonuclease I P00639 DNAS1_BOVIN 1DNK Ribonuclease H-like motif and DNase I-like SupFam Endonuclease/Exonuclease/phosphatase family 9469931 Two distinct structural folds
3.1.21.1 Deoxyribonuclease I P25736 END1_ECOLI 1OUP His-Me finger endonucleases SupFam Endonuclease I 7867949
74 3.1.21.2 Deoxyribonuclease IV (phage-T4-induced) P0A6C1 END4_ECOLI 1QUM TIM beta/alpha-barrel SupFam Xylose isomerase-like TIM barrel (clan) 10458614 Two distinct structural folds
3.1.21.2 Deoxyribonuclease IV (phage-T4-induced) P00641 ENRN_BPT7 1M0D Restriction endonuclease-like SupFam Phage endonuclease I 12093751
75 3.1.22.4 Crossover junction endodeoxyribonuclease P0A814 RUVC_ECOLI 1HJR Ribonuclease H-like motif SupFam RuvC 7923356, 1661673 Two distinct structural folds
3.1.22.4 Crossover junction endodeoxyribonuclease P0AG74 RUSA_ECOLI 1Q8R Bacillus chorismate mutase-like SupFam RusA 14656440, 9169457
76 3.1.26.4 Calf thymus ribonuclease H P10442 RNH2_ECOLI 2ETJ Ribonuclease H-like motif SupFam RNase HII 2172991 Two distinct structural folds
3.1.26.4 Calf thymus ribonuclease H P13319 RNH_BPT4 1TFR PIN_domain-like (catalytic domain) SupFam T4 RNase H, C terminal 1703156, 8674116
77 3.2.1.1 Alpha-amylase P25718 AMY1_ECOLI similar to 1EA9 TIM beta/alpha-barrel and Glycosyl hydrolase domain SupFam Alpha-amylase 9268356 Two distinct structural folds
3.2.1.1 Alpha-amylase P49067 AMYA_PYRFU similar to 1K1X 7-stranded beta/alpha barrel SupFam Glycosyl hydrolase family 57 8226990
78 3.2.1.4 Cellulase P10476 GUNA_PSEFL N-term is similar to 1UT9 Alpha/alpha toroid SupFam Glycosyl hydrolase 9 family (cellulase E) 2851699 At least four distinct structural folds
3.2.1.4 Cellulase P17901 GUNA_CLOCE 1EDG TIM beta/alpha-barrel SupFam Beta-glycanases 1744052, 8535787
3.2.1.4 Cellulase P33682 GUN1_STRHA 2BOD 7-stranded beta/alpha barrel SupFam Glycosyl hydrolases family 6 1400190
3.2.1.4 Cellulase P55742 GUNM_CLOTM 2FVG Phosphorylase/hydrolase-like SupFam Peptidase M42 GenBank: 1097207
3.2.1.4 Cellulase P22669 GUN_ASPAC 1KS4 Concanavalin A-like lectins/glucanases SupFam Glycosyl hydrolase 11 family (cellulase G) 2249253, 7586029, 2379837
3.2.1.4 Cellulase Q05622 GUNE_RUMFL similar to 1L0H Predicted: Acyl carrier protein-like SupFam Pfam hit 8360615
79 3.2.1.8 Endo-1,4-ß-xylanase P23551 XYNA_BUTFI 1XAS TIM beta/alpha-barrel SupFam Beta-glycanases 2198249, 1909424 Two distinct structural folds
3.2.1.8 Endo-1,4-ß-xylanase P33558 XYNA2_CLOSR 1QH6 Concanavalin A-like lectins/glucanases SupFam Glycosyl hydrolase 11 family (cellulase G) 7763496
80 3.2.1.14 Chitinase P07254 CHIA_SERMA 1RD6 TIM beta/alpha-barrel (catalytic domain) SupFam Type II chitinase 16453672 Two distinct structural folds
3.2.1.14 Chitinase P24626 CHI1_ORYSA 2BAA Lysozyme-like SupFam Family 19 glycosidase 1893114
81 3.2.1.17 Lysozyme P34020 LYS_CLOAB N-term is similar to 1JFX TIM beta/alpha-barrel SupFam 1,4-beta-N-acetylmuraminidase 1599233, 7649184 At least two distinct structural folds
3.2.1.17 Lysozyme Q37875 LYS_BPP1 1XJT Lysozyme-like SupFam Phage lysozyme 8576044, 9514719
3.2.1.17 Lysozyme P10773 LYB_BACSU - - SupFam B-enzyme 3148618
82 3.2.1.20 Alpha-glucosidase Q02751 MALT_CANAL 1JI1 TIM beta/alpha-barrel and Glycosyl hydrolase domain SupFam Glycosyl hydrolase family 13 1400249 Two distinct structural folds
3.2.1.20 Alpha-glucosidase O33830 AGLA_THEMA 1OBB Rossmann-fold and LDH C-terminal domain-like SupFam Glycosyl hydrolase family 4 12062450, 10972187
83 3.2.1.22 Alpha-galactosidase P27756 AGAL_STRMU 1R46 TIM beta/alpha-barrel SupFam Glycosyl hydrolase family 36 1649890 Two distinct structural folds
3.2.1.22 Alpha-galactosidase P06720 AGAL_ECOLI similar to 1OBB Rossmann-fold and LDH C-terminal domain-like SupFam Glycosyl hydrolase family 4 2831880
84 3.2.1.35 Hyaluronoglucosaminidase Q12891 HYAL2_HUMAN 1FCQ TIM beta/alpha-barrel SupFam Glycosyl hydrolase family 56 9712871, 17503783 Two distinct structural folds
3.2.1.35 Hyaluronoglucosaminidase Q54699 HYLP2_BPH45 2PK1 Triple-stranded beta-helix SupFam Hyaluronidase_1 7622224
85 3.2.1.37 Xylan 1,4-ß-xylosidase P07129 XYNB_BACPU 1YIF 5-bladed beta-propeller SupFam Glycosyl hydrolase family 43 2440680, 7766665 At least two distinct structural folds
3.2.1.37 Xylan 1,4-ß-xylosidase P36906 XYNB_THESA 1PX8 TIM beta/alpha-barrel SupFam Glycosyl hydrolase family 39 8612648
3.2.1.37 Xylan 1,4-ß-xylosidase P45702 XYLA1_BACST - - SupFam Glycosyl hydrolase family 52 8074507, 11322943
86 3.2.1.39 Glucan endo-1,3-ß-D-glucosidase P33157 E13A_ARATH 2CYG TIM beta/alpha-barrel SupFam Glycosyl hydrolase family 17 11405630, 16421930 At least two distinct structural folds
3.2.1.39 Glucan endo-1,3-ß-D-glucosidase P23903 E13B_BACCI C-term is 2VY0 Predicted: Concanavalin A-like lectins/glucanases SupFam Glycosyl hydrolase family 16 2311931, 19154353
3.2.1.39 Glucan endo-1,3-ß-D-glucosidase P22222 E13B_CELCE - - SupFam Glycosyl hydrolase family 64 1985933
87 3.2.1.49 Alpha-N-acetylgalactosaminidase P17050 NAGAB_HUMAN 3H53 TIM beta/alpha-barrel and Glycosyl hydrolase domain SupFam Melibiase 19683538 Two distinct structural folds
3.2.1.49 Alpha-N-acetylgalactosaminidase A4Q8F7 GH109_FLAME 2IXA Rossmann-fold SupFam Oxidoreductase family 17401360
88 3.2.1.55 Alpha-N-arabinofuranosidase P53627 ABFA_STRLI 2C7F TIM beta/alpha-barrel SupFam Glycosyl hydrolase family 51 8092996, 16336192 Two distinct structural folds
3.2.1.55 Alpha-N-arabinofuranosidase P45982 XYLB_BUTFI 1YRZ 5-bladed beta-propeller and Concanavalin A-like lectins/glucanases SupFam Glycosyl hydrolase family 43 1905520, 18980579
89 3.2.1.73 Licheninase P07979 GUB_NICPL 2CYG TIM beta/alpha-barrel SupFam Glycosyl hydrolase family 17 9452466 Two distinct structural folds
3.2.1.73 Licheninase P04957 GUB_BACSU 1GBG Concanavalin A-like lectins/glucanases SupFam Glycosyl hydrolase family 16 1740123
3.2.1.73 Licheninase P19254 GUB_BACCI 1V5C Alpha/alpha toroid SupFam Glycosyl hydrolase family 8 (cellulase D) 2377467
90 3.2.1.86 6-phospho-ß-glucosidase P11988 BGLB_ECOLI 1QOX TIM beta/alpha-barrel SupFam Glycosyl hydrolase family 1 3034860 Two distinct structural folds
3.2.1.86 6-phospho-ß-glucosidase P17411 CHBF_ECOLI 1S6Y Rossmann-fold and LDH C-terminal domain-like SupFam Glycosyl hydrolase family 4 10572139
91 3.2.1.91 Cellulose 1,4-ß-cellobiosidase P0C2S1 CELK_CLOTM 1UT9 Alpha/alpha toroid SupFam Glycosyl hydrolase 9 (cellulase E) family 10464199 Two distinct structural folds
3.2.1.91 Cellulose 1,4-ß-cellobiosidase P50401 GUXA_CELFI similar to 1BVW 7-stranded beta/alpha barrel SupFam Glycosyl hydrolase 6 (cellulase B) family 8065260
3.2.1.91 Cellulose 1,4-ß-cellobiosidase P07986 GUX_CELFI 1FH7 TIM beta/alpha-barrel SupFam Glycosyl hydrolase 10 (cellulase F) family 9537990
92 3.2.2.21 DNA-3-methyladenine glycosylase II P04395 3MG2_ECOLI 1MPG DNA-glycosylase and TBP-like SupFam AlkA N-terminal domain and HhH-GPD 6389535 Two distinct structural folds
3.2.2.21 DNA-3-methyladenine glycosylase II P29372 3MG_HUMAN 1F4R (C-term) FMT C-terminal domain-like SupFam Methylpurine-DNA glycosylase 8589517, 1645538
93 3.5.1.1 Asparaginase P38986 ASPG1_YEAST 1HFW Glutaminase/Asparaginase SupFam Glutaminase/Asparaginase 8026756 At least two distinct structural folds
3.5.1.1 Asparaginase P20933 ASPG_HUMAN 1APY Ntn hydrolase-like SupFam (Glycosyl)asparaginase 1840528
3.5.1.1 Asparaginase Q9RFN5 Q9RFN5_RHIET - - SupFam L-asparaginase II 10930734
94 3.5.1.4 Amidase P27765 AMID_PSECL 1OCK Amidase signature SupFam Amidase 2013568 Two distinct structural folds
3.5.1.4 Amidase P11436 ALAM_PSEAE 2UXY Carbon-nitrogen hydrolase SupFam Carbon-nitrogen hydrolase 87219101
95 3.5.1.9 Arylformamidase Q8K4H1 AFMID_MOUSE 2PBL Alpha/beta hydrolase SupFam Pfam hit 12007602, 15935693 Two distinct structural folds
3.5.1.9 Arylformamidase P0C8P4 KYNB_RALME similar to 1R61 The "swivelling" beta/beta/alpha domain SupFam Putative cyclase 14592712, 14592712
96 3.5.1.49 Formamidase Q50228 FMDA_METME similar to 2F4L CUB-like SupFam Acetamidase/formamidase 8841393 Two distinct structural folds
3.5.1.49 Formamidase O25836 AMIF_HELPY 2DYU Carbon-nitrogen hydrolase SupFam Carbon-nitrogen hydrolase 11359566, 17307742, 14742519
97 3.5.1.52 Peptide-N4-(N-acetyl-ß-glucosaminyl)asparagine amidase Q02890 PNG1_YEAST 1X3W Cysteine proteinases SupFam Transglutaminase-like 10831608, 11259433, 14726951, 15964983 At least two distinct structural folds
3.5.1.52 Peptide-N4-(N-acetyl-ß-glucosaminyl)asparagine amidase P21163 PNGF_ELIMR 1PNG Nucleoplasmin-like/VP SupFam Peptide-N-glycosidase F 7918386, 7493989
3.5.1.52 Peptide-N4-(N-acetyl-ß-glucosaminyl)asparagine amidase P81898 PNAA_PRUDU - - SupFam Patent US 5710016 (GenBank AAC20834) 9523720
98 3.5.2.6 ß-lactamase P00811 AMPC_ECOLI 2BLS Beta-lactamase/transpeptidase-like SupFam Beta-Lactamase/D-ala carboxypeptidase 6795623 Two distinct structural folds
3.5.2.6 ß-lactamase P14488 BLAB_BACCE 1BC2 Metallo-hydrolase/oxidoreductase SupFam Zn metallo-beta-lactamase 9730812
99 3.5.2.17 Hydroxyisourate hydrolase O32142 HIUH_BACSU similar to 1OO2 Prealbumin-like SupFam HIUase/Transthyretin 16098976, 16782815 Two distinct structural folds
3.5.2.17 Hydroxyisourate hydrolase Q8S3J3 HIUH_SOYBN similar to 1cbg TIM beta/alpha-barrel SupFam Glycosyl hydrolase family 1 10567345, 12481089
100 3.5.4.1 Cytosine deaminase P25524 CODA_ECOLI 1R9Z TIM beta/alpha-barrel (catalytic domain) SupFam Amidohydrolase 11812140, 1640834 Two distinct structural folds
3.5.4.1 Cytosine deaminase Q12178 FCY1_YEAST 1P6O Cytidine deaminase-like SupFam Cytidine and deoxycytidylate deaminase 9000374
101 3.5.4.3 Guanine deaminase P76641 GUAD_ECOLI 2UZ9 TIM beta/alpha-barrel (catalytic domain) SupFam Amidohydrolase 10913105 Two distinct structural folds
3.5.4.3 Guanine deaminase O34598 GUAD_BACSU 1WKQ Cytidine deaminase-like SupFam Cytidine and deoxycytidylate deaminase 15159585, 15180998, 11101664
102 3.5.4.9 Methenyltetrahydrofolate cyclohydrolase P24186 FOLD_ECOLI 1B0A Aminoacid dehydrogenase-like N-terminal domain and Rossmann-fold SupFam Tetrahydrofolate dehydrogenase/cyclohydrolase 1748668, 10386884 Two distinct structural folds
3.5.4.9 Methenyltetrahydrofolate cyclohydrolase Q49135 FCHA_METEA similar to 1O5H Methenyltetrahydrofolate cyclohydrolase-like SupFam Formiminotransferase-cyclodeaminase 10215859
103 3.5.4.10 IMP cyclohydrolase P31939 PUR9_HUMAN 1PKX Methylglyoxal synthase-like (IMP cyclohydrolase domain ) SupFam AICARFT/IMPCHase 8567683, 14756553, 14966129 Two distinct structural folds
3.5.4.10 IMP cyclohydrolase A0B9N7 PURO_METTP 2NTK Ntn hydrolase-like SupFam IMP cyclohydrolase-like (archaeal ) 17407260, 12012346
104 3.5.99.6 Glucosamine-6-phosphate deaminase P0A759 NAGB_ECOLI 1FS5 NagB/RpiA/CoA transferase-like SupFam Glucosamine_iso 1734962, 8747459 Two distinct structural folds
3.5.99.6 Glucosamine-6-phosphate deaminase Q5JDU3 Q5JDU3_PYRKO 2CB0 SIS domain SupFam SIS domain 16199574, 16857666, 17387737
105 3.6.1.1 Inorganic diphosphatase P21216 IPYR_ARATH 1TWL OB-fold SupFam Pyrophosphatase 654155 At least three distinct structural folds
3.6.1.1 Inorganic diphosphatase P31414 AVP1_ARATH - Integral membrane protein SupFam Inorganic H+ pyrophosphatase 9268385
3.6.1.1 Inorganic diphosphatase P37487 PPAC_BACSU 1WPM DHH phosphoesterases SupFam Mn-dependent inorganic pyrophosphatase 9845334, 11697905
3.6.1.1 Inorganic diphosphatase Q9JMQ2 PPAX_BACSU similar to 2HDO Predicted: HAD-like SupFam HAD-like hydrolase 12359880
106 3.6.1.5 Apyrase O96559 APY_CIMLE 2H2N 5-bladed beta-propeller SupFam Apyrase 9804829, 15006348 At least two distinct structural folds
3.6.1.5 Apyrase P50635 APY_AEDAE similar to 2Z1A 5-nucleotidase and Metallo-dependent phosphatases SupFam 5'-nucleotidase 2173922
3.6.1.5 Apyrase O75355 ENTP3_HUMAN 3CJ1, similar to 1T6C Predicted: Ribonuclease H-like motif SupFam Actin-like ATPase domain 9675246, 8955160
107 3.6.1.11 Exopolyphosphatase P29014 PPX_ECOLI 1U6Z Ribonuclease H-like motif and HD-domain/PDEase-like SupFam Ppx associated domain 8212131 Two distinct structural folds
3.6.1.11 Exopolyphosphatase P38698 PPX1_YEAST 2QB6, similar to 1K20 DHH phosphoesterases SupFam Mn-dependent inorganic pyrophosphatase 7860598
108 3.6.1.13 ADP-ribose diphosphatase O94759 TRPM2_HUMAN similar to 1QVJ NUDIX SupFam NUDIX domain (paper) 11385575 Two distinct structural folds
3.6.1.13 ADP-ribose diphosphatase Q5M886 ADPRM_RAT 2NXF Metallo-dependent phosphatases SupFam Calcineurin-like phosphoesterase 18352857
109 3.6.1.17 Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) P49776 APH1_SCHPO 1FHI HIT-like SupFam HIT family 8554540 At least two distinct structural folds
3.6.1.17 Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) P50583 AP4A_HUMAN 1XSA NUDIX SupFam MutT-like 7487923
3.6.1.17 Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) O31614 PRPE_BACSU similar to 1G5B Predicted: Metallo-dependent phosphatases SupFam Protein serine/threonine phosphatase 12059787
110 3.6.1.23 DUTP diphosphatase P33316 DUT_HUMAN 1Q5H Beta-clip SupFam dUTPase 8805593, 8631816 Two distinct structural folds
3.6.1.23 DUTP diphosphatase O15923 O15923_TRYCR (DUT) 1OGK all-alpha NTP pyrophosphatases SupFam dUTPase_2 14725764, 12208522
111 3.6.1.45 UDP-sugar diphosphatase P07024 USHA_ECOLI 2USH Metallo-dependent phosphatases and 5'-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain SupFam 5'-nucleotidase 11491293, 10331872 Two distinct structural folds
3.6.1.45 UDP-sugar diphosphatase O95848 NUD14_HUMAN similar to 1VIU NUDIX SupFam NUDIX hydrolase 12429023
112 3.8.1.3 Haloacetate dehalogenase Q01398 DEH1_MORSB 1G5F Alpha/beta hydrolase SupFam Haloalkane dehalogenase; pdb and 1IZ7, pdb and 1MJ5 1512562, 11939779 Two distinct structural folds
3.8.1.3 Haloacetate dehalogenase Q01399 DEH2_MORSB 1JUD HAD-like SupFam HAD-related 8702766
113 4.1.1.17 Ornithine decarboxylase P24169 DCOS_ECOLI 1ORD PLP-dependent transferase-like and Ornithine decarboxylase C-terminal domain SupFam Orn/Lys/Arg decarboxylase, class I 7563080 Two distinct structural folds
4.1.1.17 Ornithine decarboxylase P11926 DCOR_HUMAN 1D7K TIM beta/alpha-barrel SupFam Orn/Lys/Arg decarboxylase, class II 2587220, 2318872
114 4.1.1.18 Lysine decarboxylase P0A9H3 LDCI_ECOLI similar to 1ORD PLP-dependent transferase-like and Ornithine decarboxylase C-terminal domain SupFam Orn/Lys/Arg decarboxylase class-I 4204273 Two distinct structural folds
4.1.1.18 Lysine decarboxylase O50657 DCLO_SELRU similar to 7ODC TIM beta/alpha-barrel SupFam Orn/Lys/Arg decarboxylase class-II 11073919
115 4.1.1.19 Arginine decarboxylase P28629 ADIA_ECOLI similar to 1C4K PLP-dependent transferase-like and Ornithine decarboxylase C-terminal domain SupFam Orn/Lys/Arg decarboxylase, class I 4204273 At least two distinct structural folds
4.1.1.19 Arginine decarboxylase P21170 SPEA_ECOLI similar to 1TWI Predicted: TIM beta/alpha barrel SupFam Orn/Lys/Arg decarboxylase class II 8383109, 19298070
4.1.1.19 Arginine decarboxylase Q57764 PDAD_METJA 1N2M Pyruvoyl-dependent histidine and arginine decarboxylases SupFam Pyruvoyl-dependent ArgDC 11980912
116 4.2.1.1 Carbonate dehydratase P17582 CYNT_ECOLI similar to 1EKJ Resolvase-like SupFam Beta-carbonic anhydrase 1740425 Two distinct structural folds
4.2.1.1 Carbonate dehydratase P00915 CAH1_HUMAN 2FOY Carbonic anhydrase SupFam Eukaryotic-type carbonic anhydrase 804171, 4207120
4.2.1.1 Carbonate dehydratase P40881 CAH_METTE 1QQ0 Single-stranded left-handed beta-helix SupFam Gamma-carbonic anhydrase-like 8041719
117 4.2.1.2 Fumarate hydratase P05042 FUMC_ECOLI 1FUO L-aspartase-like SupFam L-aspartase/fumarase 8909293, 3282546 Two distinct structural folds
4.2.1.2 Fumarate hydratase P00923 FUMA_ECOLI C-term domain is 2ISB The "swivelling" beta/beta/alpha domain SupFam Class I fumarase 1917897
118 4.2.1.10 3-dehydroquinate dehydratase P43877 AROQ_ACTPL 1UQR Flavodoxin-like SupFam Type II 3-dehydroquinate dehydratase 14993670, 8170389, 2848727 Two distinct structural folds
4.2.1.10 3-dehydroquinate dehydratase P05194 AROD_ECOLI 1QFE TIM beta/alpha-barrel SupFam Class I aldolase 3541912
119 4.2.1.17 Enoyl-CoA hydratase P21177 FADB_ECOLI 1WDK ClpP/crotonase, Rossmann-fold and Thiolase-like SupFam Enoyl-CoA hydratase/isomerase family 3286611 Two distinct structural folds
4.2.1.17 Enoyl-CoA hydratase P96807 ECH1_MYCTU 2C2I Thioesterase/thiol ester dehydrase-isomerase SupFam MaoC dehydratase 16963641
120 4.2.1.78 Norcoclaurine synthase A2A1A0 NCS1_COPJA 1GP4 Double-stranded beta-helix SupFam 2OG-Fe(II) oxygenase superfamily 17204481 Two distinct structural folds
4.2.1.78 Norcoclaurine synthase Q67A25 NCS_THLFG, NCS2_COPJA 2VNE, similar to 1FM4 TBP-like SupFam Pathogenesis-related protein 17696451, 18391427
121 4.2.1.92 Hydroperoxide dehydratase Q96242 CP74A_ARATH 2RCH Cytochrome P450 SupFam Cytochrome P450 8756596, 10420644 Two distinct structural folds
4.2.1.92 Hydroperoxide dehydratase O16025 AOSL_PLEHO 1U5U Heme-dependent catalase-like SupFam Lipoxygenase 10559269, 9302294
122 4.2.2.2 Pectate lyase P0C1C2 PEL3_ERWCA 1PLU Single-stranded right-handed beta-helix SupFam Pectate lyase C 12226275 Two distinct structural folds
4.2.2.2 Pectate lyase P14005 PELP_PECCC similar to 1NXC Alpha/alpha toroid SupFam Periplasmic pectate lyase 2695748, 17881361
123 4.2.99.18 DNA-(apurinic or apyrimidinic site) lyase P20625 END3_ECOLI 2ABK DNA-glycosylase SupFam Endonuclease III 2669955 Four distinct structural folds
4.2.99.18 DNA-(apurinic or apyrimidinic site) lyase P27695 APEX1_HUMAN 1E9N DNase I-like SupFam DNase I-like 1383925, 1380694
4.2.99.18 DNA-(apurinic or apyrimidinic site) lyase P22936 APN1_YEAST similar to 1QTW TIM beta/alpha-barrel SupFam Endonuclease IV 3056935
4.2.99.18 DNA-(apurinic or apyrimidinic site) lyase P05523 FPG_ECOLI 1K82 MutM-like DNA repair protein SupFam Middle domain of MutM-like DNA repair proteins 11106507, 14607836, 11912217
124 4.3.1.17 L-serine ammonia-lyase (was 4.2.1.13) P16095 SDHL_ECOLI 2IQQ (N-term) FwdE/GAPDH domain-like SupFam Fe-S dependent L-serine dehydratase 2504697, 8436113, 8385012 Two distinct structural folds
4.3.1.17 L-serine ammonia-lyase (was 4.2.1.13) P20132 SDHL_HUMAN 1P5J Tryptophan synthase beta subunit-like PLP-dependent enzymes SupFam Serine/threonine dehydratase 14596599, 14646100, 2674117
125 4.6.1.1 Adenylate cyclase Q08828 CYA1_HUMAN 1CS4 P-loop containing NTPases and Ferredoxin-like SupFam Adenylate/guanylate cyclase (class III AC) 8314585 At least three distinct structural folds
4.6.1.1 Adenylate cyclase O69199 O69199_AERHY 2ACA CYTH-like phosphatases SupFam CYTH domain 9642185, 16905149
4.6.1.1 Adenylate cyclase P15318 CYAA_BORPE N-term is 1YRU EF Hand-like SupFam Anthrax toxin LF (class II AC) 2897067, 16138079
4.6.1.1 Adenylate cyclase P00936 CYAA_ECOLI - - SupFam Adenylate_cyclase class I 6344011, 92011391
126 5.1.1.1 Alanine racemase P0A6B4 ALR1_ECOLI 2RJG Domain of alpha and beta subunits of F1 ATP synthase-like and TIM beta/alpha-barrel SupFam Alanine racemase 18434499 Two distinct structural folds
5.1.1.1 Alanine racemase Q9UW18 TOXG_COCCA similar to 1M6S PLP-dependent transferase-like SupFam Beta-eliminating lyase 10671527
127 5.1.1.13 Aspartate racemase P83989 Q2L695_ANABR (RACD_ANABR) similar to 1WTC Tryptophan synthase beta subunit-like PLP-dependent enzymes SupFam Pyridoxal-phosphate dependent enzyme 12568809, 16452311 Two distinct structural folds
5.1.1.13 Aspartate racemase O58403 O58403_PYRHO 1JFL ATC-like SupFam Asp/Glu/Hydantoin racemase 12051922
128 5.2.1.8 Peptidylprolyl isomerase P0AFL3 PPIA_ECOLI 1CLH Cyclophilin-like SupFam Cyclophilin 9501079, 17909185 Two distinct structural folds
5.2.1.8 Peptidylprolyl isomerase P45523 FKBA_ECOLI 1Q6H FKBP-like SupFam FKBP-type PPIase 1379319
129 5.3.1.6 Ribose-5-phosphate isomerase P0A7Z0 RPIA_ECOLI 1LKZ NagB/RpiA/CoA transferase-like and Ferredoxin-like SupFam Ribose 5-phosphate isomerase 12211039, 8366047 Two distinct structural folds
5.3.1.6 Ribose-5-phosphate isomerase P37351 RPIB_ECOLI 1NN4 Ribose/Galactose isomerase RpiB/AlsB SupFam Ribose/Galactose Isomerase 14499611, 8576032
130 5.3.1.8 Mannose-6-phosphate isomerase Q59935 MANA_STRMU 1QWR Double-stranded beta-helix SupFam RmlC-like cupins 8293960 At least two distinct structural folds
5.3.1.8 Mannose-6-phosphate isomerase Q4JCA7 PGMI_SULAC similar to 1TZB SIS domain SupFam SIS_domain 14551194
5.3.1.8 Mannose-6-phosphate isomerase P29954 MANA_RHIME similar to 2GZ6 Predicted: Alpha/alpha toroid SupFam GlcNAc 2-epimerase 1452036
131 5.3.1.9 Glucose-6-phosphate isomerase P06744 G6PI_HUMAN 1JLH SIS domain SupFam Phosphoglucose isomerase 12573240 Two distinct structural folds
5.3.1.9 Glucose-6-phosphate isomerase P84140 G6PI_THELI 1J3P Double-stranded beta-helix SupFam Glucose-6-phosphate isomerase (GPI) (Archaeal-type GPI) 12560104
132 5.3.3.2 Isopentenyl-diphosphate delta-isomerase P50740 IDI2_BACSU 1P0K TIM beta/alpha-barrel SupFam FMN-dependent dehydrogenase 15206931, 12798687 Two distinct structural folds
5.3.3.2 Isopentenyl-diphosphate delta-isomerase Q46822 IDI_ECOLI 1HZT NUDIX SupFam NUDIX 10419945, 11285217
133 5.3.3.10 5-carboxymethyl-2-hydroxymuconate delta-isomerase Q05354 HPCD_ECOLX 1OTG Tautomerase/MIF SupFam CHMI, 5-carboxymethyl-2-hydroxymuconate isomerase 8547259, 2194841 Two distinct structural folds
5.3.3.10 5-carboxymethyl-2-hydroxymuconate delta-isomerase P37352 HPCE_ECOLX 1I7O FAH SupFam Fumarylacetoacetate (FAA) hydrolase 8223600
134 5.3.99.2 Prostaglandin-D synthase O60760 PTGD2_HUMAN 1IYH Thioredoxin fold and GST C-terminal domain-like SupFam Glutathione S-transferase 9353279, 10824118 Two distinct structural folds
5.3.99.2 Prostaglandin-D synthase P41222 PTGDS_HUMAN 2CZT Lipocalins SupFam Lipocalin family 8336134, 9475419
135 5.3.99.3 Prostaglandin-E synthase Q9N0A4 PGES2_MACFA 1Z9H Thioredoxin fold and GST C-terminal domain-like SupFam Glutathione S-transferase 15854652, 11866447 Two distinct structural folds
5.3.99.3 Prostaglandin-E synthase O14684 PTGES_HUMAN 2H8A MAPEG domain-like SupFam MAPEG family 12672824, 12460774
5.3.99.3 Prostaglandin-E synthase Q15185 TEBP_HUMAN 1EJF HSP20-like chaperones SupFam CS domain 10922363, 10811660
136 5.4.2.1 Phosphoglycerate mutase P31217 GPMA_ECOLI 1E59 Phosphoglycerate mutase-like SupFam Phosphoglycerate mutase 11038361 Two distinct structural folds
5.4.2.1 Phosphoglycerate mutase P52832 GPMI_PSESM 1EJJ Alkaline phosphatase-like SupFam 2,3-Bisphosphoglycerate-independent phosphoglycerate mutase 12076796
137 5.4.2.8 Phosphomannomutase P26405 RFBK_SALTY 1K2Y Phosphoglucomutase SupFam Phosphoglucomutase 1710759 Two distinct structural folds
5.4.2.8 Phosphomannomutase Q92871 PMM1_HUMAN 2FUC HAD-like SupFam Eukaryotic phosphomannomutase 9119384
138 5.4.99.5 Chorismate mutase P42517 CHMU_ENTAG 2AO2 Chorismate mutase II SupFam Secreted chorismate mutase-like 8335631, 9497350, 7496534 Two distinct structural folds
5.4.99.5 Chorismate mutase P19080 CHMU_BACSU 1COM Bacillus chorismate mutase-like SupFam Chorismate mutase 8378335
139 5.5.1.1 Muconate cycloisomerase P08310 CATB_PSEPU 1MUC TIM beta/alpha-barrel and Enolase N-terminal domain-like SupFam D-glucarate dehydratase-like 3609743, 3612800 Two distinct structural folds
5.5.1.1 Muconate cycloisomerase P46057 MLE_TRICU C-term is similar to C-term of 1JOF 7-bladed beta-propeller SupFam WD40/YVTN repeat 8110801, 11937053
140 5.99.1.2 DNA topoisomerase P06612 TOP1_ECOLI 1CY1 Prokaryotic type I DNA topoisomerase SupFam Prokaryotic type I DNA topoisomerase 3029379, 8114910, 7779808 Two distinct structural folds
5.99.1.2 DNA topoisomerase P11387 TOP1_HUMAN 1LPQ DNA breaking-rejoining enzymes SupFam Eukaryotic DNA topoisomerase I 98155246, 8747458, 16352556
141 6.1.1.6 Lysine—tRNA ligase P13030 SYK1_ECOLI 1BBU Class II aaRS and biotin synthetases SupFam Class II aminoacyl-tRNA synthetase 7735833, 11887185, 2183178 Two distinct structural folds
6.1.1.6 Lysine—tRNA ligase Q9YFT9 SYK_AERPE 1IRX Adenine nucleotide alpha hydrolase-like SupFam Class I lysyl-tRNA synthetase 9353192, 11887185, 16529662
No. EC no. Enzyme name NCBI UniProt PDB SCOP fold Super-family Family (Pfam) Ref. Predicted
142 1.2.1.31 L-aminoadipate-semialdehyde dehydrogenase P49419 AL7A1_HUMAN 2J6L, similar to 1EUH Predicted: ALDH-like SupFam Aldehyde dehydrogenase 16491085, 18694748 Predicted analogs
1.2.1.31 L-aminoadipate-semialdehyde dehydrogenase P07702 LYS2_YEAST similar to 2VSQ Predicted: CoA-dependent acyltransferases, Acetyl-CoA synthetase-like and Rossmann-fold SupFam NAD_binding_4 10320345, 12756539 Predicted analogs
143 1.3.1.9 Enoyl-[acyl-carrier-protein] reductase (NADH) P0A5Y6 INHA_MYCTU 2NV6 Rossmann-fold SupFam Short-chain dehydrogenase 10336454 Predicted analogs
1.3.1.9 Enoyl-[acyl-carrier-protein] reductase (NADH) P07149 FAS1_YEAST 3HMJ Predicted: TIM beta/alpha-barrel SupFam Enoyl-[acyl-carrier-protein] reductase (Fungal fatty acid synthetase subunit beta) 17431175, 19679086 Predicted analogs
144 1.3.1.20 Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase Q7JK39 DHDH_MACFU 2O48 Predicted: Rossmann-fold and FwdE/GAPDH domain-like SupFam Oxidoreductase family 11306093, 10477285, 17654552 Predicted analogs
1.3.1.20 Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase Q04828 AK1C1_HUMAN 1MRQ TIM beta/alpha-barrel SupFam Aldo/keto reductase 11013348, 8573067 Predicted analogs
145 1.5.1.3 Dihydrofolate reductase P11045 DYR_BACSU 1MVS Dihydrofolate reductase-like SupFam Dihydrofolate reductases 1731871 Predicted analogs
1.5.1.3 Dihydrofolate reductase P0AFS3 FOLM_ECOLI similar to 3edm Predicted: Rossmann-fold SupFam Short chain dehydrogenase 14617668 Predicted analogs
146 1.14.13.39 Nitric-oxide synthase P29476 NOS1_RAT 1TLL Reductase/isomerase/elongation factor common domain and Flavodoxin-like and Ferredoxin reductase-like C-terminal NADP-linked domain SupFam NOS family 1379068 Predicted analogs
1.14.13.39 Nitric-oxide synthase Q66GP9 NOS1_ARATH similar to 3EC1 Predicted: P-loop SupFam Plant NOS family 14526079, 11916378 Predicted analogs
147 2.1.1.17 Phosphatidylethanolamine N-methyltransferase Q05197 PMTA_RHOSH similar to 3DH0 Predicted: SAM-dependent methyltransferases SupFam SAM-dependent methyltransferase 8340421 Predicted analogs
2.1.1.17 Phosphatidylethanolamine N-methyltransferase P05374 PEM1_YEAST - Integral membrane protein SupFam Pfam hit 2445736 Predicted analogs
148 2.1.1.31 tRNA (guanine-N1-)-methyltransferase P0A873 TRMD_ECOLI 1P9P Alpha/beta knot SupFam TRNA (Guanine-1)-methyltransferase 6337136, 14583191 Predicted analogs
2.1.1.31 tRNA (guanine-N1-)-methyltransferase Q32P41 TRMT5_HUMAN C-term is similar to N-term of 2FRN Predicted: S-adenosyl-L-methionine-dependent methyltransferases SupFam Met-10+ like-protein 15248782 Predicted analogs
149 2.3.1.1 Amino-acid N-acetyltransferase P0A6C5 ARGA_ECOLI 2R8V Acyl-CoA N-acyltransferases (Nat) SupFam Acetyltransferase (GNAT) family 16890 Predicted analogs
2.3.1.1 Amino-acid N-acetyltransferase Q04728 ARGJ_YEAST 1VZ6 DmpA/ArgJ-like SupFam ArgJ family 9428669 Predicted analogs
150 2.3.1.43 Phosphatidylcholine—sterol O-acyltransferase P04180 LCAT_HUMAN 1ex9 Alpha/beta hydrolase SupFam Lecithin:cholesterol acyltransferase 2823898 Predicted analogs
2.3.1.43 Phosphatidylcholine—sterol O-acyltransferase P10480 GCAT_AERHY similar to: 1PP4 Flavodoxin-like SupFam Lipase_GDSL 3280033 Predicted analogs
151 2.3.1.51 1-acylglycerol-3-phosphate O-acyltransferase O15120 PLCB_HUMAN similar to 1K30 Predicted: Glycerol-3-phosphate (1)-acyltransferase SupFam Acyltransferase 9242711 Predicted analogs
2.3.1.51 1-acylglycerol-3-phosphate O-acyltransferase Q12385 ICT1_YEAST similar to 1IUN Predicted: Alpha/beta hydrolase SupFam Alpha/beta hydrolase 18252723 Predicted analogs
152 2.3.1.84 Alcohol O-acetyltransferase P40353 ATF1_YEAST similar to 1L5A Predicted: CoA-dependent acyltransferases SupFam Alcohol acetyltransferase 8085822, 7764365 Predicted analogs
2.3.1.84 Alcohol O-acetyltransferase P38295 MCFS2_YEAST similar to 1VA4 Predicted: Alpha/beta hydrolase SupFam Abhydrolase_1 16361250 Predicted analogs
153 2.7.4.21 Inositol-hexakisphosphate kinase Q12494 KCS1_YEAST 1W2D SAICAR synthase-like SupFam Inositol phosphokinase 15350214 Predicted analogs
2.7.4.21 Inositol-hexakisphosphate kinase Q06685 VIP1_YEAST similar to 1GSA Predicted: ATP-grasp SupFam Histidine acid phosphatase 17412958, 17690096 Predicted analogs
154 3.1.1.1 Carboxylesterase O33407 ESTA_PSEAE similar to 1PP4 Predicted: Flavodoxin-like SupFam GDSL-like Lipase/Acylhydrolase 10559163 Predicted analogs
3.1.1.1 Carboxylesterase P13001 BIOH_ECOLI 1M33 Alpha/beta hydrolase SupFam Alpha/beta hydrolase 12732651 Predicted analogs
155 3.1.1.47 1-alkyl-2-acetylglycerophosphocholine esterase Q13093 PAFA_HUMAN 3D59 Predicted: Alpha/beta hydrolase SupFam Serine esterase 8624782, 18784071 Predicted analogs
3.1.1.47 1-alkyl-2-acetylglycerophosphocholine esterase P68401 PA1B2_BOVIN (beta) and PA1B3_BOVIN (gamma) 1FXW Flavodoxin-like (beta and gamma) SupFam GDSL' lipolytic enzyme 8083218, 8985254 Predicted analogs (cytosolic ezyme is formed of three subunits)
156 3.1.2.2 Palmitoyl-CoA hydrolase O55137 ACOT1_MOUSE 3HLK Predicted: Alpha/beta hydrolase SupFam BAAT / Acyl-CoA thioester hydrolase C terminal 10567408 Predicted analogs
3.1.2.2 Palmitoyl-CoA hydrolase O00154 BACH_HUMAN 1YLI, 4HBT? Predicted: Thioesterase/thiol ester dehydrase-isomerase SupFam Thioesterase superfamily 10578051 Predicted analogs
157 3.1.2.14 Oleoyl-[acyl-carrier-protein] hydrolase P08635 SAST_RAT similar to 1C4X Predicted: Alpha/beta hydrolase SupFam Thioesterase 3105579 Predicted analogs
3.1.2.14 Oleoyl-[acyl-carrier-protein] hydrolase P07149 FAS1_YEAST 1S9C Thioesterase/thiol ester dehydrase-isomerase SupFam Acyl transferase 3528750, 3031066 Predicted analogs
158 3.1.3.1 Alkaline phosphatase P00634 PPB_ECOLI 1ED8 Alkaline phosphatase-like SupFam Alkaline phosphatase 2010919 Predicted analogs
3.1.3.1 Alkaline phosphatase Q05205 PPB_LYSEN similar to C-term of 1XZW Predicted: Metallo-dependent phosphatases SupFam Calcineurin-like phosphoesterase 1856159 Predicted analogs
159 3.1.3.3 Phosphoserine phosphatase Q9UBQ0 VPS29_HUMAN 1W24 Metallo-dependent phosphatases SupFam Calcineurin-like phosphoesterase 16737443 Predicted analogs
3.1.3.3 Phosphoserine phosphatase O07014 RSBP_BACSU similar to 1TXO Predicted: PP2C-like SupFam Protein phosphatase 2C 10632888 Predicted analogs
3.1.3.3 Phosphoserine phosphatase O82796 SERB_ARATH similar to 1NNL HAD-like SupFam SerB family 10196182 Hydrolyzes free Ser~P
160 3.1.3.7 3'(2'),5'-bisphosphate nucleotidase Q42546 DPNP1_ARATH similar to 1QGX Carbohydrate phosphatase SupFam Inositol monophosphatase 17993620, 10205895 Predicted analogs
3.1.3.7 3'(2'),5'-bisphosphate nucleotidase O34600 NRNA_BACSU similar to 1K20 Predicted: DHH phosphoesterases SupFam DHH 17586819 Predicted analogs
161 3.1.3.27 Phosphatidylglycerophosphatase P18200 PGPA_ECOLI similar to 1RFZ Predicted: YutG-like SupFam Phosphatidyl-glycerophosphatase A 6296050 Predicted analogs
3.1.3.27 Phosphatidylglycerophosphatase P0A924 PGPB_ECOLI similar to 1D2T Predicted: Acid phosphatase/Vanadium-dependent haloperoxidase SupFam Phosphatidic acid phosphatase (PAP2) 6296050 Predicted analogs
162 3.1.4.3 Phospholipase C P15713 PHLN_PSEAE 2D1G Predicted: Alkaline phosphatase-like SupFam Phosphoesterase 2120196 Predicted analogs, by VAST
3.1.4.3 Phospholipase C Q0TV31 PHLC_CLOP1 1QM6 Phospholipase C/P1 nuclease SupFam Zinc dependent phospholipase C 11008117, 12009886 Predicted analogs
163 3.1.4.4 Phospholipase D P20626 PLD_CORPS - Predicted: TIM beta/alpha-barrel (only motif) SupFam Pfam hit 2407718, 7934899 Predicted analogs
3.1.4.4 Phospholipase D Q53728 PLD_STRAT 1F0I Phospholipase D/nuclease SupFam Phospholipase D Active site motif 7765769, 10089437, 18338352 Predicted analogs
164 3.1.4.12 Sphingomyelin phosphodiesterase P17405 ASM_HUMAN similar to 2AHD Predicted: Metallo-dependent phosphatases SupFam Calcineurin-like phosphoesterase 1840600, 12631268 Predicted analogs (acid)
3.1.4.12 Sphingomyelin phosphodiesterase Q6UWV6 ENPP7_HUMAN similar to 2GS0 PH domain-like barrel SupFam Type I phosphodiesterase / nucleotide pyrophosphatase 12885774 Predicted analogs (alkaline)
3.1.4.12 Sphingomyelin phosphodiesterase O60906 NSMA_HUMAN similar to 1I9Y Predicted: DNase I-like SupFam Endonuclease/Exonuclease/phosphatase 10608884 Predicted analogs (neutral)
3.1.4.12 Sphingomyelin phosphodiesterase Q9NXE4 NSMA3_HUMAN - - SupFam Pfam hit 16517606 Predicted analogs (neutral)
165 3.1.4.16 2',3'-cyclic-nucleotide 2'-phosphodiesterase Q2FZ08 CNPD_STAA8 - Predicted: HD-domain/PDEase-like SupFam 2',3' cyclic nucleotide phosphodiesterase 17951247, 15853881 Predicted analogs
3.1.4.16 2',3'-cyclic-nucleotide 2'-phosphodiesterase P08331 CN16_ECOLI similar to 2USH 5-nucleotidase and Metallo-dependent_phosphatases SupFam 5'-nucleotidase 3005231 Predicted analogs
166 3.1.4.17 3',5'-cyclic-nucleotide phosphodiesterase Q07343 PDE4B_HUMAN 1XLX HD-domain/PDEase-like SupFam Cyclic nucleotide phosphodiesterase 9371714, 8384210 Predicted analogs
3.1.4.17 3',5'-cyclic-nucleotide phosphodiesterase P22434 PDE1_YEAST similar to 1P9E Predicted: Metallo-hydrolase/oxidoreductase SupFam cAMP phosphodiesterase class-II 2824992 Predicted analogs
167 3.1.4.35 3',5'-cyclic-GMP phosphodiesterase B0G0Y8 PDE3_DICDI 2HD1 HD-domain/PDEase-like SupFam 3'5'-cyclic nucleotide phosphodiesterase 11171061, 17040207 Predicted analogs
3.1.4.35 3',5'-cyclic-GMP phosphodiesterase P12019 PDE1_DICDI similar to: 1KO2 Predicted: Metallo-hydrolase/oxidoreductase SupFam cAMP phosphodiesterases class-II 12429832 Predicted analogs
168 3.1.4.53 3',5'-cyclic-AMP phosphodiesterase Q23917 PDE2_DICDI 2HD1 HD-domain/PDEase-like SupFam 3'5'-cyclic nucleotide phosphodiesterase 9812977, 10488068 Predicted analogs
3.1.4.53 3',5'-cyclic-AMP phosphodiesterase P12019 PDE1_DICDI similar to: 1KO2 Predicted: Metallo-hydrolase/oxidoreductase SupFam cAMP phosphodiesterases class-II 12429832 Predicted analogs
169 3.1.13.4 Poly(A)-specific ribonuclease Q504Q3 PAN2_HUMAN 1WLJ (domain) Ribonuclease H-like motif SupFam DnaQ-like 3'-5' exonuclease, Exonuc X-T 14583602 Predicted analogs
3.1.13.4 Poly(A)-specific ribonuclease P31384 CCR4_YEAST similar to 1AKO Predicted: DNase I-like SupFam Endonuclease/Exonuclease/phosphatase 11889048, 11889047 Predicted analogs
170 3.2.1.3 Glucan 1,4-alpha-glucosidase P69328 AMYG_ASPNG 1GAI Alpha/alpha toroid SupFam Glycosyl hydrolases family 15 1527049, 8503847, 3081341 Predicted analogs
3.2.1.3 Glucan 1,4-alpha-glucosidase O43451 MGA_HUMAN 2QLY Predicted: TIM beta/alpha-barrel SupFam Glycosyl hydrolases family 31 18036614, 3143729 Predicted analogs
171 3.2.1.11 Dextranase P48845 DEXT_PENMI 1OGM Dextranase N-terminal domain and Single-stranded right-handed beta-helix SupFam Glycosyl hydrolases family 28 8905923, 12962629 Predicted analogs
3.2.1.11 Dextranase P39653 DEXT_STRDO similar to: 1EA9 Predicted: TIM beta/alpha-barrel SupFam Glycosyl hydrolase 66 family 8021165, 12030973 Predicted analogs
172 3.2.1.45 Glucosylceramidase P04062 GLCM_HUMAN 2V3D TIM beta/alpha-barrel SupFam O-Glycosyl hydrolase family 30 17666401 Predicted analogs
3.2.1.45 Glucosylceramidase Q9HCG7 GBA2_HUMAN similar to 2JG0 Predicted: Alpha/alpha toroid SupFam DUF608, Protein of unknown function 11489889 Predicted analogs
173 3.2.1.51 Alpha-L-fucosidase P04066 FUCO_HUMAN 1ODU Glycosyl_hydrolase_domain and TIM_beta/alpha-barrel SupFam Glycosyl hydrolase 29 family 2894306, 2174090 Predicted analogs
3.2.1.51 Alpha-L-fucosidase Q9FXE5 FUCO3_ARATH Predicted: 1ESC Predicted: Flavodoxin-like SupFam GDSL' lipolytic enzyme 11788770 Predicted analogs
3.2.1.51 Alpha-L-fucosidase Q8L7W8 FUCO2_ARATH 2RDY Predicted: Alpha/alpha toroid SupFam Glycosyl hydrolase 95 family 15262925 Predicted analogs
174 3.2.1.58 Glucan 1,3-ß-glucosidase P29717 EXG_CANAL 1CZ1 TIM beta/alpha-barrel SupFam Glycosyl hydrolase family 5 (cellulase A) 8436950, 10610795 Predicted analogs
3.2.1.58 Glucan 1,3-ß-glucosidase P49426 EXG1_COCCA 3EQN, 1KTW Predicted: Single-stranded right-handed beta-helix SupFam Glycosyl hydrolase family 55 8135518 Predicted analogs
175 3.2.1.81 ß-agarase P07883 AGAR_STRCO - Predicted: Concanavalin A-like lectins/glucanases SupFam Glycosyl hydrolase family 16 3034860 Predicted analogs
3.2.1.81 ß-agarase P48839 AGAA_VIBS7 - TIM beta/alpha-barrel SupFam Glycosyl hydrolase family 50 8285681 Predicted analogs
176 3.2.1.96 Mannosyl-glycoprotein endo-ß-N-acetylglucosaminidase P59206 LYTB_STRR6 similar to 2ZYC Predicted: Lysozyme-like SupFam Glucosaminidase 10096093 Predicted analogs
3.2.1.96 Mannosyl-glycoprotein endo-ß-N-acetylglucosaminidase P36912 EBA2_FLAME similar to 1EOK Predicted: TIM beta/alpha-barrel SupFam Glycosyl hydrolase 18 family (UniProt) 8486657, 7768917 Predicted analogs
177 3.2.2.22 rRNA N-glycosylase P02879 RICI_RICCO 1APG Ribosome inactivating proteins (RIP) SupFam Ribosome inactivating protein 1433290, 8780513 Predicted analogs
3.2.2.22 rRNA N-glycosylase Q1JPL7 PME18_ARATH Predicted: 1X8Z, 1GQ8 Predicted: Bromodomain-like (N-term) and Single-stranded right-handed beta-helix (C-term) SupFam PMEI, Plant invertase/pectin methylesterase inhibitor and pectinesterase 18222123 Predicted analogs
178 3.5.1.28 N-acetylmuramoyl-L-alanine amidase P13016 AMPD_ECOLI 1J3G N-acetylmuramoyl-L-alanine amidase-like SupFam N-acetylmuramoyl-L-alanine amidase 2 12654266 Predicted analogs
3.5.1.28 N-acetylmuramoyl-L-alanine amidase P26365 AMIB_ECOLI C-term is similar to 1JWQ Predicted: Phosphorylase/hydrolase-like SupFam Zn-dependent exopeptidases 7511774 Predicted analogs
179 3.6.1.6 Nucleoside-diphosphatase Q9WUZ9 ENTP5_MOUSE similar to N-term of 3CJ1 Predicted: Ribonuclease H-like motif SupFam GDA1/CD39 NTPase 10369669 Predicted analogs
3.6.1.6 Nucleoside-diphosphatase Q8WVQ1 CANT1_HUMAN 2H2N 5-bladed beta-propeller SupFam Apyrase 12167635 Predicted analogs
180 4.1.1.22 Histidine decarboxylase P28577 DCHS_ENTAE similar to 1JS3 Predicted: PLP-dependent transferase-like SupFam Pyridoxal-dependent decarboxylase 2033044 Predicted analogs
4.1.1.22 Histidine decarboxylase P00862 DCHS_LACS3 1HQ6 Pyruvoyl-dependent histidine and arginine decarboxylases SupFam Histidine decarboxylase 2857718 Predicted analogs
181 4.2.2.3 Poly(ß-D-mannuronate) lyase P59786 ALGL_PSEFL similar to 1QAZ Predicted: Alpha/alpha toroid SupFam Chondroitin AC/alginate lyase 12775688 Predicted analogs
4.2.2.3 Poly(ß-D-mannuronate) lyase Q59478 ALYA_KLEPN similar to 1VAV Predicted: Concanavalin A-like lectins/glucanases SupFam Concanavalin A-like lectins/glucanases 8200539 Predicted analogs
4.2.2.3 Poly(ß-D-mannuronate) lyase Q06365 ALYP_PSESO N-term is similar to 1OFM Predicted: Single-stranded right-handed beta-helix SupFam Polysaccharide lyase 6 (UniProt) 8336113, 8319887 Predicted analogs
182 4.2.2.9 Pectate disaccharide-lyase Q05526 PELW_DICD3 2V8J, similar to 1FP3 Predicted: Alpha/alpha toroid SupFam Periplasmic pectate lyase 10383957, 1766386, 17881361 Predicted analogs
4.2.2.9 Pectate disaccharide-lyase P22751 PELX_ERWCH C-term is similar to C-term of 1RU4 Predicted: Single-stranded right-handed beta-helix SupFam Polysaccharide lyase 9 (UniProt) 10383957, 2254266 Predicted analogs
183 4.3.1.18 D-serine ammonia-lyase P00926 SDHD_ECOLI similar to 1O58 Tryptophan synthase beta subunit-like PLP-dependent enzymes SupFam Pyridoxal-phosphate dependent enzyme 6853521, 10601247 Predicted analogs
4.3.1.18 D-serine ammonia-lyase P53095 YGU6_YEAST Predicted: 1L6G Predicted: TIM beta/alpha-barrel SupFam Alanine racemase, N-terminal domain 17937657, 18564178 Predicted analogs
184 4.99.1.4 Sirohydrochlorin ferrochelatase P0AEA8 CYSG_ECOLI 1PJT Rossmann-fold, Siroheme synthase middle domains-like and Tetrapyrrole methylase SupFam Siroheme synthase (multi doman) 8243665, 2407558, 2407234 Predicted analogs
4.99.1.4 Sirohydrochlorin ferrochelatase P61817 SIRB_BACME Predicted: 1tjn Predicted: Chelatase-like SupFam CbiX family, SirB subfamily 12408752 Predicted analogs
185 5.3.3.1 Steroid delta-isomerase P00947 SDIS_COMTE 1OCV Cystatin-like SupFam Nuclear transport factor 2 4753764, 10551849 Predicted analogs
5.3.3.1 Steroid delta-isomerase P14060 3BHS1_HUMAN similar to 1EQ2 Predicted: Rossmann-fold SupFam 3-beta hydroxysteroid dehydrogenase/isomerase 2139411, 2082186, 1401999 Predicted analogs
186 5.3.3.12 L-dopachrome isomerase P40126 TYRP2_HUMAN similar to 1WX2, 1LNL Predicted: Di-copper centre-containing domain SupFam Tyrosinase 8573077 Predicted analogs
5.3.3.12 L-dopachrome isomerase P34884 MIF_MOUSE 2GDG Tautomerase/MIF SupFam Macrophage migration inhibitory factor (MIF) 7947826, 10360941, 8900542, 9794786 Predicted analogs
187 no EC 4-Methyl-5-hydroxyethylthiazole phosphate synthase P30139 THIG_ECOLI 1XM3 TIM beta/alpha-barrel SupFam Thiazole biosynthesis protein 17403671, 15012138 Predicted analogs (no EC)
no EC 4-Methyl-5-hydroxyethylthiazole phosphate synthase P32318 THI4_YEAST 2GJC FAD/NAD(P)-binding domain SupFam Thi4 family 9367751, 12794638, 18652458 Predicted analogs (no EC)
No. EC no. Enzyme name NCBI UniProt PDB SCOP fold Super-family Family (Pfam) Ref. Potential
188 1.1.1.81 Hydroxypyruvate reductase P70788 TTUD3_AGRVI similar to 2B8N GckA/TtuD-like SupFam MOFRL 8672817, 7592429 Potential analogs (mis-annotation)
1.1.1.81 Hydroxypyruvate reductase P37666 GHRB_ECOLI similar to 2DBR, 1GDH Predicted: Rossmann-fold and Flavodoxin-like SupFam D-isomer specific 2-hydroxyacid dehydrogenase 11237876 Potential analogs
189 1.1.5.2 Quinoprotein glucose dehydrogenase P05465 DHGA_ACICA similar to 1KV9 8-bladed beta-propeller and Cytochrome c SupFam Bacterial PQQ dehydrogenase 3399393, 16754970 Potential analogs (related structures)
1.1.5.2 Quinoprotein glucose dehydrogenase P13650 DHGB_ACICA 1CRU 6-bladed beta-propeller SupFam PQQ oxidoreductase gdhB 14741705 Potential analogs (related structures)
190 1.4.1.2 Glutamate dehydrogenase P41755 DHE2_ACHKL - - SupFam NAD-specific glutamate dehydrogenase 8308022 Potential analogs (no papers after 1994)
1.4.1.2 Glutamate dehydrogenase P39633 DHE2_BACSU 1GTM Rossmann-fold and Aminoacid dehydrogenase-like N-terminal domain SupFam Glu/Leu/Phe/Val dehydrogenases family 9829940 Potential analogs
191 1.13.11.15 3,4-dihydroxyphenylacetate 2,3-dioxygenase Q45135 Q45135_9MICO 1F1X Glyoxalase/Bleomycin resistance protein/ Dihydroxybiphenyl dioxygenase SupFam Glyoxalase 9179284, 15028678, 15568806, 16217642 Potential analogs
1.13.11.15 3,4-dihydroxyphenylacetate 2,3-dioxygenase Q05353 HPCB_ECOLI - - SupFam LigB 3053656, 2261999 Potential analogs (Fe dependent)
192 1.14.11.16 Peptide-aspartate ß-dioxygenase Q9NWT6 HIF1N_HUMAN 1H2K Double-stranded beta-helix and Transactivation domain SupFam JmjC domain 12042299, 12080085, 12432100 Potential analogs
1.14.11.16 Peptide-aspartate ß-dioxygenase Q12797 ASPH_HUMAN - - SupFam Aspartyl/asparaginyl beta-hydroxylase 7821814 Potential analogs
193 1.14.13.7 Phenol 2-monooxygenase P19730, P19731 DMPL_PSEUF
DMPM_PSEUF		 2INP, 1HQI Ferritin-like and beta-Grasp (ubiquitin-like) and Monooxygenase (hydroxylase) regulatory protein SupFam Methane/Phenol/Toluene Hydroxylase 17176061, 2254258 Potential analogs
1.14.13.7 Phenol 2-monooxygenase P15245 PH2M_TRICU 1FOH FAD/NADP-binding domain, Thioredoxin fold and FAD-linked_reductases C-terminal domain SupFam Pfam hit 8145253, 9634698 Potential analogs
194 1.14.15.3 Alkane 1-monooxygenase P12691 ALKB_PSEOL - - SupFam Fatty acid desaturase 2647718 Potential analogs
1.14.15.3 Alkane 1-monooxygenase Q5TCH4 CP4AM_HUMAN similar to 1TQN Cytochrome P450 SupFam Cytochrome P450 14641109, 7798189, 11931657, 2361958 Potential analogs
195 2.3.1.23 1-acylglycerophosphocholine O-acyltransferase O42916 ALE1_SCHPO - - SupFam MBOAT family, integral membrane protein 17890783 Potential analogs
2.3.1.23 1-acylglycerophosphocholine O-acyltransferase Q06510 TAZ1_YEAST similar to 1IUQ Predicted: Glycerol-3-phosphate (1)-acyltransferase SupFam Acyltransferase 15588229, 16135531 Potential analogs
196 2.3.1.75 Long-chain-alcohol O-fatty-acyltransferase Q93ZR6 WSD1_ARATH - - SupFam N-term UPF0089 family 18621978 Potential analogs (activity?)
2.3.1.75 Long-chain-alcohol O-fatty-acyltransferase Q9XGY6 WAXS1_SIMCH - - SupFam Wax synthase 10712527 Potential analogs
2.3.1.75 Long-chain-alcohol O-fatty-acyltransferase Q58HT5 AWAT1_HUMAN - - SupFam Diacylglycerol acyltransferase 15671038 Potential analogs
197 2.3.1.88 Peptide alpha-N-acetyltransferase P41227 ARD1A_HUMAN similar to 1MK4 Acyl-CoA N-acyltransferases (Nat) SupFam GNAT superfamily 7981673, 15496142 Potential analogs
2.3.1.88 Peptide alpha-N-acetyltransferase P37293 NAT2_YEAST - - SupFam No Pfam hits 8175741, 9128142 Potential analogs
198 2.3.2.13 Protein-glutamine gamma-glutamyltransferase P00488 F13A_HUMAN, TGAS_STRMB 1FIE, 1IU4 Cysteine proteinases SupFam Transglutaminase, microbial transglutaminase 9839945, 2901091, 2877456 Potential analogs
2.3.2.13 Protein-glutamine gamma-glutamyltransferase P40746 TGL_BACSU - - SupFam Bacillus TGase 9692191 Potential analogs
199 2.4.1.69 Galactoside 2-alpha-L-fucosyltransferase Q9TUD4 FUT1_GORGO - - SupFam Glycosyltransferase family 11 10723735, 7372640, 8613146 Potential analogs
2.4.1.69 Galactoside 2-alpha-L-fucosyltransferase Q9M5Q1 FUT1_PEA - - SupFam Xyloglucan fucosyltransferase (Glycosyltransferase family 37) 10747946 Potential analogs
2.4.1.69 Galactoside 2-alpha-L-fucosyltransferase Q54QG0 PGFB_DICDI N-term is similar to 1H7Q Predicted: Nucleotide-diphospho-sugar transferases (N-term) SupFam Glycosyltransferase family 2 (UniProt) 11423539, 12244067 Potential analogs
200 2.4.1.226 N-acetylgalactosaminyl-proteoglycan 3-ß-glucuronosyltransferase Q8IZ52 CHSS2_HUMAN - - SupFam Chondroitin N-acetylgalactosaminyl-transferase 12761225 Potential analogs
2.4.1.226 N-acetylgalactosaminyl-proteoglycan 3-ß-glucuronosyltransferase Q8L0V4 CHS_ECOLX 2Z86 Predicted: Nucleotide-diphospho-sugar transferases (N-term and C-term) SupFam Glycosyltransferase 2 11943778 Potential analogs
201 2.7.7.24 Glucose-1-phosphate thymidylyltransferase P61887 RMLA2_ECOLI 1MC3 Nucleotide-diphospho-sugar transferases SupFam Nucleotidyl transferase 7559340, 12171937 Potential analogs
2.7.7.24 Glucose-1-phosphate thymidylyltransferase P29785 STRO_STRGR Predicted: 1LBV Predicted: Carbohydrate phosphatase SupFam Inositol monophosphatase family 1661369 Potential analogs (activity?)
202 2.7.7.50 mRNA guanylyltransferase Q01159 MCE1_YEAST 1P16 ATP-grasp (catalytic domain) SupFam Eukaryotic GTase 6389537, 3029058, 6094533 Potential analogs
2.7.7.50 mRNA guanylyltransferase P11079 LMBD2_REOVD 1EJ6 Low-resolution structure 1EJ6 does not allow comparison SupFam Orthoreovirus lambda-2 10801118 Potential analogs
2.7.7.50 mRNA guanylyltransferase A2T3S5 VP3_ROTSH - - SupFam Rotavirus VP3 10603323 Potential analogs
203 2.7.8.5 CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase P25578 PGPS1_YEAST Predicted: 1BYS Predicted: Phospholipase D/ nuclease SupFam Phospholipase D (CDP-alcohol phosphatidyltransferase class-I, UniProt) 9545322 Potential analogs
2.7.8.5 CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase P0ABF8 PGSA_ECOLI - - SupFam CDP-alcohol phosphatidyltransferase 1323047, 8195097 Potential analogs
204 2.7.8.8 CDP-diacylglycerol-serine O-phosphatidyltransferase P23830 PSS_ECOLI similar to 1BYR Predicted: Phospholipase D/ nuclease SupFam Phospholipase D Active site motif 1323044, 10074947 Potential analogs
2.7.8.8 CDP-diacylglycerol-serine O-phosphatidyltransferase P08456 PSS_YEAST - Integral membrane protein SupFam CDP-alcohol phosphatidyltransferase 3040403 Potential analogs
205 2.7.13.3 Histidine kinase P0AEJ4 ENVZ_ECOLI 1BXD ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase SupFam Histidine kinase domain 10426948, 1323560 Potential analogs
2.7.13.3 Histidine kinase Q9AWA5 GWD1_SOLTU - - SupFam Pyruvate phosphate dikinase, PEP/pyruvate binding domain 12011472 Potential analogs (chloroplast precursor)
206 3.1.1.32 Phospholipase A1 P0A921 PA1_ECOLI 1QD5 Transmembrane beta-barrels SupFam Phospholipase A1 8300539 Potential analogs
3.1.1.32 Phospholipase A1 P18952 PA1_SERLI - Predicted: Alpha/beta hydrolase SupFam Pfam hit 3056919 Potential analogs
207 3.1.3.6 3'-nucleotidase P0A840 SURE_ECOLI 1ILV SurE-like SupFam SurE nucleotidase 15489502 Potential analogs
3.1.3.6 3'-nucleotidase P24504 NUP3_PENSQ 1AK0 Phospholipase C/P1 nuclease SupFam S1/P1 Nuclease 1964878, 1369324 Potential analogs
20 3.1.3.78 Phosphatidylinositol-4,5-bisphosphate 4-phosphatase Q86T03 TM55B_HUMAN) - - SupFam Transmembrane protein 55A 16365287 Potential analogs
3.1.3.78 Phosphatidylinositol-4,5-bisphosphate 4-phosphatase Q07566 IPGD_SHIFL - - SupFam Enterobacterial virulence protein IpgD 12356723, 11029686 Potential analogs
209 3.2.1.132 Chitosanase Q9P961 Q9P961_ASPOR - - SupFam Fungal chitosanase 11388486 Potential analogs
3.2.1.132 Chitosanase O07921 CHIS_BACSU 1CHK Lysozyme-like SupFam Glycosyl hydrolase family 46 10658653, 10521473 Potential analogs
210 3.2.1.143 Poly(ADP-ribose) glycohydrolase Q9NX46 ARHL2_HUMAN 2FOZ, similar to 1T5J ADP-ribosylglycohydrolase SupFam ADP-ribosylglycohydrolase 16278211 Potential analogs
3.2.1.143 Poly(ADP-ribose) glycohydrolase Q86W56 PARG_HUMAN - - SupFam Poly (ADP-ribose) glycohydrolase (PARG) 10449915, 9115250 Potential analogs
211 3.2.2.5 NAD+ nucleosidase (ADP ribosyl cyclase) P28907 CD38_HUMAN 2EF1 Flavodoxin-like SupFam Ribosyl hydrolyase 8253715, 16154090 Potential analogs
3.2.2.5 NAD+ nucleosidase (ADP ribosyl cyclase) A2RCA5 A2RCA5_STRPG - - SupFam NAD glycohydrolase 15627505, 16177331, 18048938 Potential analogs
212 3.5.1.23 Ceramidase Q13510 ASAH1_HUMAN Predicted: 3PVA Predicted: Ntn hydrolase-like SupFam Choloylglycine hydrolase 7744740, 8955159, 10993717 Potential analogs (acid)
3.5.1.23 Ceramidase Q5QJU3 ASA3L_HUMAN - - SupFam Alkaline phytoceramidase 11356846 Potential analogs (alkaline)
3.5.1.23 Ceramidase Q9NR71 ASAH2_HUMAN similar to 2ZXC - SupFam Neutral/alkaline non-lysosomal ceramidase 10781606, 16229686 Potential analogs (neutral)
213 3.6.1.27 Undecaprenyl-diphosphatase P80143 UDDP_SULAC C-term is similar to C-term of 1QI9 Predicted: Acid phosphatase/ Vanadium-dependent haloperoxidase SupFam PAP2 superfamily 1321720 Potential analogs
3.6.1.27 Undecaprenyl-diphosphatase P60932 UPPP_ECOLI - - SupFam Bacitracin resistance protein BacA 15778224 Potential analogs
No. EC no. Enzyme name and REACTION NCBI UniProt PDB SCOP fold Super-family Family (Pfam) Ref. Comments
214 4.1.1.11 Aspartate 1-decarboxylase (L-Aspartate <=> ß-Alanine + CO2) P0A790 PAND_ECOLI 1AW8 Double psi beta-barrel SupFam Aspartate decarboxylase 6767707 Two distinct structural folds
4.1.1.15 Glutamate decarboxylase (L-Aspartate <=> ß-Alanine + CO2) Q05329 DCE2_HUMAN 1JS3 PLP-dependent transferase-like SupFam Pyridoxal-dependent decarboxylase 6956856, 658037
215 1.4.3.21 Primary-amine oxidase (Tyramine + H2O + Oxygen <=> 4-Hydroxyphenylacetaldehyde + NH3 + H2O2) P49250 AMO_KLEAE 1OAC Supersandwich and Cystatin-like and N domain of copper amine oxidase-like SupFam Copper amine oxidase 1556068 Two distinct structural folds
1.4.3.4 Amine oxidase (flavin-containing) (Tyramine + H2O + Oxygen <=> 4-Hydroxyphenylacetaldehyde + NH3 + H2O2) Q6NSN2 AOF_DANRE 1O5W FAD/NADP-binding domain and FAD-linked reductases C-terminal domain SupFam Flavin monoamine oxidase 16917825
216 2.7.4.14 Cytidylate kinase (ATP + UMP <=> ADP + UDP) Q5EBM0 CMPK2_HUMAN similar to 2TMK P-loop containing NTPases SupFam Thymidylate kinase 17999954 Two distinct structural folds
2.7.4.22 UMP kinase (ATP + UMP <=> ADP + UDP) P0A7E9 PYRH_ECOLI 2BNE Carbamate kinase-like SupFam Amino acid kinase 7711027
217 3.1.3.62 multiple inositol-polyphosphate phosphatase (myo-Inositol hexakisphosphate + H2O <=> 1L-myo-Inositol 1,2,3,4,6-pentakisphosphate + Orthophosphate) O35217 MINP1_RAT similar to 1QWO Phosphoglycerate mutase-like SupFam Multiple inositol-polyphosphate phosphatase 9359836 Two distinct structural folds
3.1.3.72 5-phytase; Systematic name: myo-inositol-hexakisphosphate 5-phosphohydrolase (myo-Inositol hexakisphosphate + H2O <=> 1L-myo-Inositol 1,2,3,4,6-pentakisphosphate + Orthophosphate) Q7WUJ1 Q7WUJ1_SELRU 1U24 Phosphotyrosine protein phosphatases II SupFam Pfam hit 17567745
218 3.2.1.93 Alpha,-alpha-phosphotrehalase (H2O + alpha,alpha'-Trehalose 6-phosphate <=> D-Glucose + D-Glucose 6-phosphate) P39795 TREC_BACSU 1UOK TIM beta/alpha-barrel and Glycosyl hydrolase domain SupFam Alpha amylase 7751281 Two distinct structural folds
3.2.1.122 Maltose-6'-phosphate glucosidase (H2O + alpha,alpha'-Trehalose 6-phosphate <=> D-Glucose + D-Glucose 6-phosphate) O06901 MALH_FUSMR 1U8X LDH C-terminal domain-like and Rossmann-fold SupFam Family 4 glycosyl hydrolase 11882720, 7730284
219 3.5.1.2 Glutaminase (L-Glutamine + H2O <=> L-Glutamate + NH3) Q9UI32 GLSL_HUMAN 3CZD Beta-lactamase/ transpeptidase-like SupFam Glutaminase 18459799 Two distinct structural folds
3.5.1.38 Glutamin-(asparagin-)ase (L-Glutamine + H2O <=> L-Glutamate + NH3) Q88K39 ASPQ_PSEPK, ASPG2_ECOLI 1DJO Glutaminase/ Asparaginase SupFam Asparaginase 10684596, 8434007, 1521538
220 3.8.1.2 (S)-2-haloacid dehalogenase ((S)-2-Haloacid + H2O <=> (R)-2-Hydroxyacid + Halide) Q52087 HADL_PSEPU 1QH9 HAD-like SupFam Haloacid dehalogenase-like hydrolase 1588303 Two distinct structural folds
3.8.1.10 2-haloacid dehalogenase (configuration-inverting) ((S)-2-Haloacid + H2O <=> (R)-2-Hydroxyacid + Halide) O06652 HADDL_PSES4 3BJX - SupFam Halocarboxylic acid dehydrogenase DehI 9209038
221 5.4.2.2 Phosphoglucomutase (Alpha-D-Ribose 1-phosphate <=> D-Ribose 5-phosphate) P00949 PGM1_RABIT 1JDY Phosphoglucomutase first 3 domains and TBP-like SupFam Phosphoglucomutase/ phosphomannomutase 15299905, 1328221 Two distinct structural folds
5.4.2.7 Phosphopentomutase (Alpha-D-Ribose 1-phosphate <=> D-Ribose 5-phosphate) P0A6K6 DEOB_ECOLI 2I09 Alkaline phosphatase-like and DeoB insert domain-like SupFam Phosphopentomutase 4904508
222 1.3.1.44 Trans-2-enoyl-CoA reductase (NAD+) (Butanoyl-CoA + NAD+ <=> Crotonoyl-CoA + NADH + H+) Q5EU90 TER_EUGGR - Predicted: Rossmann-fold SupFam Short-chain alcohol dehydrogenase 15569691 Predicted analogs
1.3.99.2 Butyryl-CoA dehydrogenase (Butanoyl-CoA + NAD+ <=> Crotonoyl-CoA + NADH + H+) Q06319 ACDS_MEGEL, ACADS_HUMAN 1BUC Bromodomain-like and Acyl-CoA dehydrogenase NM domain-like SupFam Acyl-CoA dehydrogenase 8399220, 7857927 Predicted analogs
223 2.5.1.44 Homospermidine synthase (Putrescine + Spermidine <=> Homospermidine + 1,3-Diaminopropane) O32323 HSS_RHOVI 2PH5 Predicted: Rossman-fold and FwdE/GAPDH domain-like SupFam Homospermidine synthase 8841401 Predicted analogs
2.5.1.45 Homospermidine synthase (spermidine-specific) (Putrescine + Spermidine <=> Homospermidine + 1,3-Diaminopropane) Q9SC13 HSS1_SENVE 1RLZ DHS-like NAD/FAD-binding domain SupFam Deoxyhypusine synthase 10611289 Predicted analogs
224 2.7.1.31 Glycerate 2-kinase P77364 GLXK1_ECOLI 1TO6 Glycerate kinase I SupFam Glycerate kinase type-1 9772162 Two distinct structural folds
2.7.1.31 Glycerate 2-kinase Q8IVS8 GLCTK_HUMAN smilar to 2B8N GckA/TtuD-like SupFam Glycerate kinase type-2 (contains MOFRL domain) 16753811, 16865707